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Articles by Ezequiel Viveros-Valdez
Total Records ( 3 ) for Ezequiel Viveros-Valdez
  Sara Garcia-Davis , Ivan Murillo-Alvarez , Mauricio Munoz-Ochoa , Edith Carranza-Torres , Ruth Garza-Padron , Eufemia Morales-Rubio and Ezequiel Viveros-Valdez
  Background and Objective: Marine environment represents countless and diverse resource for new drugs to combat major diseases. Extracts from four Laurencia species (L. johnstonii, L. pacifica, L. gardneri and L. papillosa) from Baja California Sur, Mexico were evaluated for their antioxidant, antibacterial and cytotoxic activity. Methodology: The antioxidants activity of Laurencia sp. were evaluated using the radical scavenging activity in three in vitro radicals: 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and nitric oxide (NO). The antibacterial activity was evaluated by the broth microdilution method to determinate the Minimum Inhibitory Concentrations (MIC) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Pseudomonas aeruginosa and Klebsiella pneumoniae. The cytotoxicity was analyzed on HeLa (cervix adenocarcinoma) and Vero (kidney epithelial) cells, using the reduction of tetrazolium salt WST-1. Results: The seaweed of genus Laurencia demonstrated an overall low activity, with half maximal effective concentration (EC50) values >1.5 mg mL–1. Laurencia pacifica showed the best biocide effects with MIC of 6.25 μg mL–1 against Gram positive bacterial and cytotoxic potential with half inhibitory concentration (IC50) <30 μg mL–1 against Vero and HeLa cells. Conclusion: Some Laurencia species have a great antibacterial and cytotoxic activity which could be considered for future studies.
  Sara Garcia-Davis , Mauricio Munoz-Ochoa , Catalina Rivas-Morales and Ezequiel Viveros-Valdez
  Background and Objective: Invertebrates comprises more than fifty percent of marine animals. Among them, sponges are the most prolific source of bioactive compounds. Suberites aurantiacus (S. aurantiacus) is an abundant sponge in Mexican Pacific, which has been scarcely studied, for this reason authors screened it for its bioactivities. The current study proposed to determine the enzymatic inhibition and scavenger, toxic and antibacterial activities from Suberites aurantiacus. Materials and Methods: S. aurantiacus samples were collected in Magdalena Bay, Mexico. The ethanolic extract and its fractions were assayed for their antioxidant effect using DPPH, ABTS and NO assays, evaluated their toxicity against Artemia salina (A. salina) and their antibacterial activity against Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Enterococcus faecalis (E. faecalis), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) as well as their inhibitory effect on α-glucosidase and α-amylase. Results: The results showed median lethal doses (LD50) against A. salina < 1 mg mL–1 for two fractions and a moderate antibacterial activity against Gram positive bacteria. Conclusion: In view of these results, S. aurantiacus could be considered a potential source of antibacterial compounds.
  Yesenia Silva-Belmares , Catalina Rivas-Morales , Ezequiel Viveros-Valdez , Maria Guadalupe de la Cruz-Galicia and Pilar Carranza-Rosales
  The antimicrobial and cytotoxic activities of organic extracts obtained from roots of the medicinal plant Jatropha dioica (Euphorbiaceae) were investigated. In order to evaluate their antimicrobial activity, the organic extracts were tested against clinical isolates of the human pathogens Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Enterobacter aerogenes, Enterobacter cloacae, Salmonella typhimurium, Cryptococcus neoformans, Candida albicans, Candida parapsilosis and Sporothrix schenckii. Results revealed that the hexane extract possess the stronger activity and a broader microbicide spectrum compared to the acetone and ethanol extracts. The activity of hexane extract may be attributed in part to the presence of β-sitosterol, the major compound identified by bioautography. The hexane extract, as well as the bioactive fraction were not cytotoxic when assays were profiled against the normal cell lines Chang, OK and LLCPK-1 (IC50>1000 μg mL-1).
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