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Articles by Evana Kamarudin
Total Records ( 4 ) for Evana Kamarudin
  Nur Syahiirah Saberi , Mazura Bahari , Mohd Nazri Abu , Mohd Fahmi Mastuki , Wan Shahriman Yushdie Wan Yusoff and Evana Kamarudin
  This study aimed to determine the potential of the Mud crab (Scylla serrata) as a source to treat blood disorders. Eighteen male Wistar rats were randomly divided into three groups (2 treatment groups and 1 control group) of six rats (n = 6). All groups were kept under similar condition. Mud crab stock solutions were prepared in 7.0 mg mL–1 protein/kg and 52 mg mL–1 protein/kg doses and orally administered to treatment groups for three weeks. Distilled water was used for the control group. Blood samples were obtained from each rat via cardiac puncture for further analysis of various haematological parameters. The result revealed that the average protein content of the extract was 5.2±0.06 mg mL–1 protein/kg. RBC, Hb, HCT and MCH level increased significantly in the treatment groups compared to control group. In conclusion, mud crab contains vital nutrients that boost production of red blood cell indices, hence could serve as an essential food supplement to treat anemia.
  Wan Shahriman Yushdie Wan Yusoff , Intan Nur Shahfiqah Baharum , Evana Kamarudin , Mohd Fahmi Mastuki and Mohd Nazri Abu
  Food contaminants such as vegetable particles in cytology specimens can cause misdiagnosis by mimicking the cells and microorganisms. Misdiagnosis can contribute to false positive or false negative result which then whether will cause delays in diagnosis or an unnecessary treatment thus led to morbidity. The objective of this study is to describe the morphology of traditional salads cells in Malaysia and to identify the mimicking characters of these cells with normal, abnormal and microorganism from cytology specimens. Commonly consumed traditional salads in Malaysia were selected and homogenized using mortar and pestle. This is to resemble chewing affect by breaking down the samples into small particles. Each samples were smeared onto glass slides using back-to-back method. Prepared slides were stained with PAP and MGG stains. The cells were observed and images were recorded. Traditional salads contaminants can resemble Cocobacillus sp., Trichomonas vaginalis, Candida sp. spores, parasites ova, squamous epithelial cells, squamous glandular cells and HSIL. As a conclusion, most of traditional salads cells cytomorphology in this study can mimic human cells and microorganisms in cytology specimens. These finding will provide reference in cytology diagnostic to minimize the rate of misdiagnosis which reassure proper patient treatment.
  Mohammad Shafiq Zahari , Siti Nazrina Camalxaman , Mohd Nazri Abu , Wan Shahriman Yushdie , Wan Yusoff , Evana Kamarudin , Azlin Sham Rambely and Mohd Fahmi Mastuki
  Although, molecular detection has been found to be sensitive and specific but DNA extraction step is still essential, which is known to be time-consuming, costly and a high risk of contamination. Y-PER reagent had previously demonstrated its effectiveness in disruption of some bacterial cells for releasing genomic DNA with an extremely simple and rapid procedure. We therefore want to investigate if this reagent and technique could be applied on six common nosocomial pathogens including Escherichia coli, Staphylococccus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp. Two extraction methods were performed using boiling and Y-PER reagent technique and the resulting genomic DNA extractions were then subjected to PCR analysis by real-time multiplex PCR assay. DNA templates extracted by boiling method has shown a successful amplification for all of the six bacteria, while Y-PER technique only shows a positive amplification for Escherichia coli and Streptococcus pneumonia. Although, Y-PER method has a potential to be rapid and simple molecular diagnostic tool for releasing genomic DNA for PCR analysis, further optimization need to be done for many bacteria in order to fully utilize Y-PER as replacement for boiling method.
  Evana Kamarudin , Mohd Khairul Amran Mohammad , Tressa Gadung Saptu and Rozi Hanisa Musa
  The Rh blood group system is the most complex, polymorphic and immunogenic protein-based blood group system in humans. Some individuals produce a weak expression of D antigen on RBCs surface as a result of missense RHD mutations and amino acid substitutions that lead to distinct serologic phenotypes and anti-D immunization. This study aimed to elucidate the molecular genetic basis of weak D phenotypes in Malaysian blood donors with multiracial population. A total of 183 Rh-negative blood samples were phenotyped for D, C, c, E and e antigens. Weak D samples that identified by Indirect Antiglobulin Test (IAT) were amplified by polymerase chain reaction-sequence specific primers for weak D type determination. A total of 183 multiracial Rh-negative donors were reviewed, consisting of 88 (48.1%) Indians, 56 (30.6%) Malays, 23 (12.6%) Chinese and 16 (8.7%) other minority ethnics. Four samples were found to be positive for weak D phenotype using IAT. Of these four samples, two samples were reported with weak D type 15. Both samples were from Chinese blood donor with DweakCcee and DweakccEe phenotype, respectively. This study provide the first database of a molecular basis of weak D in Malaysian blood donors which improved the understanding of molecular mechanisms underlying D antigen expression in Malaysian population. Thus, increase the transfusion safety in highly racial mixed population.
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