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Articles by Esmaeel Fallah
Total Records ( 3 ) for Esmaeel Fallah
  Esmaeel Fallah , Behroz Mahdavi Poor , Rasol Jamali , Karim Hatam Nahavandi and Mohammad Asgharzadeh
  The aim of the present study was to determine Cryptosporidium species and genotypes in cattle by molecular methods. Fecal samples were collected from 104 adult cattle in a slaughterhouse in Tabriz, Northwestern Iran during the period from June to July 2007. Initial identification of cryptosporidiosis was carried out by formalin-ether concentration and Kinyoun acid fast staining method. Genomic DNA was extracted from microscopically positive samples and nested PCR was performed to amplify the partial small-subunit rRNA gene of Cryptosporidium that were subsequently digested by restriction enzymes to determine the Cryptosporidium species and genotypes present. In this study Cryptosporidium parasites were found in 10.5% (11 cases) adult cattle. Among 11 analyzed isolates, two different species of Cryptosporidium were identified; 64% (seven cases) of isolates belonged to C. andersoni and 36% (four cases) to the potentially zoonotic species of C. parvum bovine genotype. The results of present study showed that two species of Cryptosporidium, C. andersoni and C. parvum bovine genotype are responsible for cattle cryptosporidiosis in this region and the existence of C. parvum bovine genotype suggest that there is a potential risk of zoonotic transmission of C. parvum bovine genotype infection between cattle and human, likely by means of contaminated water or food, or through direct contact in the farmers and veterinary staff.
  Esmaeel Fallah , Kareem Hatam Nahavandi , Rasul Jamali , Behroz Mahdavi Poor and Mohammad Asgharzadeh
  The purpose of this study was to investigate the genotypes of Giardia duodenalis from human and animal feces and their epidemiological in Iran. Molecular characterization of cysts of human and animal origin represents an objective means to approve or reject this hypothesis. In this study, cysts of Giardia duodenalis were collected from feces of naturally infected cats (n = 2), human (n = 34), dog (n = 2) and cattle (n = 3). PCR-RFLP analysis of the 34 specimens recovered from humans revealed 6 G. duodenalis assemblage AII, 8 G. duodenalis assemblage BIII and 4 G. duodenalis assemblage BIV. Among samples from cats, 1 was classified into assemblage AI. Genetic subgenotypes identified from human reveals that genetic diversity of this protozoan in East Azerbaijan, Iran, is similar to that of Giardia from other parts of the world. The present study represents the first contribution to the knowledge of G. duodenalis genotypes in Iran.
  Esmaeel Fallah , Kareem H. Nahavandi , Rasul Jamali , Behroz Mahdavi and Mohammad Asgharzadeh
  In this study, 325 stool samples from sporadic cases giardiasis were examined by conventional techniques for parasite diagnosis. A simple and rapid procedure for the extraction of DNA from fecal samples was developed. Triose phosphate isomerase (tim) based PCR assay was applied for definitive identification and genetic characterization of Giardia intestinalis strains collected from Tabriz Reference Laboratory and pediatric Hospital in Tabriz. Among 34 DNA samples extracted, the tim gene was amplified from 31 (91.1%). Of these, 13 (41.9%) samples contained assemblage B, 17 (54.8%) contained assemblage A and one (3.2%) contained a mixture of assemblage A and assemblage B. Of these, three samples (8.8%) were negative. The results indicated that PCR technique provides an applicable and feasible method for detection and identification of Giardia cysts in stool samples. The results of furthermore, demonstrated that Giardia intestinalis assemblage A and B exist in East Azerbaijan province of Iran.
 
 
 
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