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Articles by Eshrat Gharaei-Fathabad
Total Records ( 3 ) for Eshrat Gharaei-Fathabad
  Eshrat Gharaei-Fathabad
  Biosurfactants can be served as green alternatives in a variety of applications including bioremediation, pharmaceuticals, agricultural disease control and cosmetics. Biosurfactant mixtures produced by microbes and they are genus- and sometimes species-specific. Because of their short fatty acid tails and polar head groups, biosurfactants are highly sticky and both hydrophilic and hydrophobic. In pharmaceutics, biosurfactants can be used for gene delivery and recovery of intracellular products as well as they can be served as antimicrobial substances and emulsifying agents.
  Eshrat Gharaei-Fathabad and Masoumeh Eslamifar
  The aim of the study was to identify the occurrence of lactic acid bacteria in tea leaves and examined the probiotic activity. Probiotics are live microorganisms that are similar to beneficial microorganisms found in the human gut. Probiotics are available to consumers mainly in the form of dietary supplements and foods. Most probiotics are bacteria and come from two groups namely Lactobacillus and Bifidobacterium. Owing to the widespread increased interest in finding probiotics from safe resources, several studies have been directed at identifying such microorganisms. In this study, the presence of probiotic microorganisms were investigated in the northern tea leaves of Iran. Samples of tea including green leaves and black tea leaves were collected and screened for identifying lactic acid flora. Microorganisms were isolated by pour plate method on MRS agar plates. The plates were incubated under aerobic and anaerobic conditions at 37°C for 48 h. One rod-shaped bacteria with the potential of lactic acid fermentation and probiotic activity was isolated from both two sources. The bacillus strain identified as Lactobacillus paraplantarum according to morphological and biochemical tests and 16s rRNA amplification. This microorganism could inhibit the growth of Salmonella typhi, E. coli, Staphylococcus aureous, Entrococcus fecalis and Citrobacter sp. successfully. Further more, this strain could produce extracellular tannase which is a beneficial property for probiotic activity. According to this study, we have concluded that, some of the important probiotic microorganisms exist in the tea leaves which could be utilized as a safe source for isolating probiotics.
  Eshrat Gharaei-Fathabad , Mojtaba Tabatabaei Yazdi , Seyed-Naser Ostad , Shadman Shokravi , Zargham Sepehrizadeh , Mohammad Ali Faramarzi and Mohsen Amini
  A total of 100 supernatants and methanol extract samples obtained from three weeks’ incubation of 50 different isolate strains of cyanobacteria with paddy-fields origin were investigated for their cytotoxicity properties. Fifteen supernatants and 10 methanol extract samples exhibited cytotoxic activity against Balb-C cells while one methanol extract sample possessed a cytotoxic effect against five different cell lines including HeLa, Vero, Caco-2, HepG-2 and CHO more than vincristine, 5-fluorouracil and methotrexate when used at their IC50 concentrations. The later potent extract was achieved from the cyanobacterium strain GT-319. It was characterized as Nostoc piscinale Kützing ex Bornet and Flahault 1888 according to the morphological studies. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing. This is the first report on the cytotoxic activity of N. piscinale GT-319.
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