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Articles by Emad I. Hussein
Total Records ( 3 ) for Emad I. Hussein
  Emad I. Hussein , Fuad A. Al-Horani and Hanan I. Malkawi
  Crude petroleum-oil is a complex mixture of hydrophobic components like n-alkanes, aromatics, resins and asphaltenes. Microorganisms are known to attack and degrade a specific component as compared with other components of oil. This study aimed at investigating the biodegradation potential of 20 bacterial consortia that were previously isolated from 20 different contaminated sites at the Aqaba region and to screen their potential to biodegrade different types of hydrocarbons and clean up oil spills in different contaminated sites in Aqaba (Jordan) in situ and ex situ. In these contaminated soil and seawater sites, the concentrations of Total Petroleum Hydrocarbon (TPH) ranged between 45000-65000 ppm in soil and 25000-35000 ppm in seawater. Twenty different bacterial consortia from soil and seawater samples of a petroleum contaminated sites were constructed and screened for their ability to degrade crude petroleum oil. In general, bacterial consortia derived from contaminated soil and seawater sites showed maximum percentage of degradation 85 and 70%, respectively, of crude oil after 11th week of incubation. However, this study demonstrated that microbial consortia or microbial population of crude oil biodegraders could be maintained for extended periods while at and still be effective in degrading crude oil or other mixtures of toxic organics even high concentrations.
  Emad I. Hussein , Jacob H. Jacob , Abdel-Salam F. Jahmani and Nigem Din Yousef
  Zarqa River (ZR) is an important river basin in Jordan. However, it is continuously exposed to different types of pollutants including heavy metals. This study was carried out to determine the concentration of the following heavy metals in this vital environment: Pb, Cd, Cu and Zn and to enumerate, isolate and characterize the indigenous bacteria inhabiting this environment to test their heavy metal tolerance and bioremoval ability. Samples in this study include four different types: water, soil, sediment and three local plants (Nerium oleander, Phragmites australis and Nicotenia glauca) from 13 sampling sites assigned as A1-A13 starting from Kherbat Al-Samra treatment plant and ending at King Talal dam. High concentration of Pb was detected in water samples from site A1 (3200 mg L-1) as well as Cd from site A13 (2500 mg L-1). In soil, Zn was found at high concentrations in all sampling sites while Cd was found at high concentration only in site A12. High concentrations of Zn and Cu were detected in sediment samples from sites A1 and A8, respectively. In case of plant samples, Zn was detected at high concentration in Nicotenia glauca. A relatively high viable bacterial counts in site A12 soil and sediment samples were detected (2x1015 and 1.8x1014 CFU mL-1, respectively) and in site A13 water samples (6x1017 CFU mL-1). Identified bacteria belong to Staphylococcus, Escherichia, Lactobacillus, Bacillus, Pseudomonas, Micrococcus, Klebsiella, Enterobacter, Alcaligenes, Mycobacterium, Citrobacter, Corynebacterium, Acetobacter, Serratia and Salmonella. Among them, Corynebacterium sp., was the most effective in heavy metal bioremoval.
  Emad I. Hussein , Ghassan J. M. Kanan , Khalid M. Al- Batayneh , Khalaf Alhussaen , Wesam Al Khateeb , Janti Qar , Jacob H. Jacob , Riyadh Muhaidat and Mohamed I. Hegazy
  The post-harvest moulds Penicillium digitatum and Penicillium italicum are important plant pathogens and spoilage-causing molds especially against citrus fruits. If not treated, post-harvest moulds can cause enormous economic losses during storage and marketing. Therefore, more investigations are needed to examine new antifungal agents against such fungi. In this work, we aimed to evaluate the antifungal activity of some plant extracts (namely, Harmal seeds (Peganum harmala L.), cinnamon bark (Cinnamomum cassia L.) and sticky fleabane leaves (Inula viscosa L.), food preservatives (namely, sodium benzoate, sodium molybdate, ammonium heptamolybdate tetrahydrate, potassium carbonate and sodium bicarbonate) and their mixtures, i.e., plant extracts and food preservatives against P. digitatum and P. italicum. Both disc agar diffusion method and broth dilution methods was used to evaluate the antifungal activity of the plant extracts and food preservatives. Results revealed that methanolic fractions of cinnamons’ bark and sticky fleabane leaves showed the highest efficacy. MIC values of 150 and 37.5 μg mL-1 were obtained with cinnamons’ fraction against P. italicum and P. digitatum, respectively. Sodium benzoate was the most effective against tested fungal species. The obtained MIC values against P. digitatum and P. italicum were 37.5 and 75 μg mL-1, respectively. Mixtures of tested chemicals showed synergistic effects against both fungal species. Mixtures of sodium benzoate and fractions of either cinnamon or sticky fleabane reflected synergistic effects against P. italicum and antagonistic effects against P. digitatum. Inhibition zones against P. italicum ranged between 38-57 mm.
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