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Articles by Elizabeth Wina
Total Records ( 2 ) for Elizabeth Wina
  Sri Suharti , Dewi Apri Astuti , Elizabeth Wina and Toto Toharmat
  This research was aimed to investigate the utilization of whole lerak extract to improve rumen fermentation, nitrogen retention and performance of beef cattle received high forage based ration. Experimental diet composed of forage (70%) and concentrate (30%). The in vivo study was conducted using 12 local beef cattle which were divided into three treatments ie three different levels of lerak extract (0, 100 and 200 mg/kg body weight) were added to the diet. Parameters measured were nutrient digestibility, volatile fatty acid (VFA) profile, NH3 concentration, microbial protein synthesis, feed intake and daily gain of beef cattle during 90 days of feeding trial. The addition of lerak extract up to the level of 200 mg/kg BW did not affect nutrient digestibility. Total VFA and propionate proportion increased (p<0.05) and the ratio of acetate: propionate decreased (p<0.05) with the addition of lerak extract. Concentration of NH3 in the rumen tended to decrease. Nitrogen retention, microbial protein synthesis, feed intake and daily gain of local beef cattle fed high forage ration tended to increase with the addition of lerak extract at the level up to 200 mg/kg BW. The addition of lerak extract at the level of 200 mg/kg BW increased average daily gain up to 12.5% compared to the control treatment.
  Wulansih D. Astuti , Komang G. Wiryawan , Elizabeth Wina , Yantyati Widyastuti , Sri Suharti and Roni Ridwan
  Background and Objective: Probiotics are widely used in ruminant production, but information about the potential of Lactobacillus plantarum (L. plantarum) as a probiotic for ruminants is still limited. The aim of this research was to select L. plantarum strains as a probiotic for ruminants and to determine their effect on the rumen fermentation system. Materials and Methods: The first experiment was conducted using a randomized block design to select 14 strains of L. plantarum isolated from rumen cattle. The second experiment was arranged in a completely randomized design using two selected L. plantarum strains to determine their effects as a probiotic on rumen fermentation. The substrates used for in vitro fermentation were napier grass (Pennisetum purpureum) and concentrate in a 70:30 ratio. Results: From experiment 1, L. plantarum U32 was selected, because it produced low methane/total gas (27.39%) and strain U40 was selected because it had the highest dry matter and organic matter rumen disappearance (56.45 and 56.44%). In experiment 2, the addition of L. plantarum U32 and U40 as probiotics increased propionic acid and decreased acetic production (p<0.05), which led to a lower A:P ratio (p<0.05). The total volatile fatty acid and in vitro digestibility were not affected by the addition of L. plantarum. Probiotic addition increased lactic acid bacteria and the protozoa population (p<0.05) from the rumen fluid compared to the control. The total rumen bacteria were not significantly changed by the treatments. Conclusion: The addition of L. plantarum strains U32 and U40 as probiotics had beneficial effects for rumen fermentation due to increased propionic acid and decreased methane production.
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