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Articles by E.T. Gonzalez
Total Records ( 2 ) for E.T. Gonzalez
  C.J. Panei , K. Suzuki , M.G. Echeverria , M.S. Serena , G.E. Metz and E.T. Gonzalez
  The objective of this preliminary study was to describe alleles of bovine major histocompatibility complex class II BoLA-DRB3.2 gene associated with resistance and susceptibility to Persistent Lymphocytosis (PL) in Bovine leukaemia virus (BLV) infected cattle, to set a direction for the future studies. A total of 81 dairy cattle in Argentina were investigated for the distribution of BoLA-DRB3.2 alleles. The study cattle were categorized into 3 groups as follows: Category PL+ (BLV seropositive with PL); Category PL¯ (BLV seropositive without PL) and Category BLV¯ (BLV seronegative). The alleles were identified by polymerase chain reaction with subsequent analysis of restriction fragment length polymorphism. The association between the BoLA-DRB3.2 alleles and the PL+ and PL¯ categories was determined as the odds ratio. Some alleles BoLA-DRB3.2 mediating (1) resistance and (2) susceptibility to PL were observed, respectively. A stochastic model including Shannon’s diversity index calculation was run based on Markov-Chain Monte Carlo simulation method. The Shannon’s diversity indices in each of the cattle categories indicated that the richness of gene diversity decreased as leading to a clinical stage of BLV. This relates to the existence of alleles mediating resistance and susceptibility to PL. The stochastic prediction with inclusion of uncertainty is important which can serve as a future study beacon.
  R. Lopez , K. Suzuki , E.T. Gonzalez , G. Ruiz , A. Loza , C. Coca , J.A.C. Pereira and M.R. Pecoraro
  This study determined seroprevalence against bovine leukaemia virus and investigated the risk factors with the seropositivity of the virus in dairy cattle in Bolivia. Ninety-eight farms in five different provinces in Santa Cruz Department were visited to study 1823 dairy cattle. Questionnaire interviews, blood sampling and inspection of skin were performed at each study farm. Individual-cattle sera were analysed using the agarose gel immunodiffusion (AGID) diagnostic method for the detection of antibody against bovine leukaemia virus. The overall percentage of test positive against bovine leukaemia virus was 29% (95% confidence interval: 27-31%). One percent of the study dairy cattle had the subcutaneous lesions. There were statistical differences for percentage of test positive against bovine leukaemia virus in dairy cattle between provinces (p<0.001). The seropositivity in relation to the existence of subcutaneous lesions indicated statistical significance (p = 0.023). Knowledge of the provincial difference of seropositivity against bovine leukaemia virus in dairy cattle would be used to determine the resource allocation for preventive measures in the study area. In the preventive measures, serological tests against bovine leukaemia virus for the cattle with subcutaneous lesions, which would be a potential indicator of the infection of bovine leukaemia, should be prioritised.
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