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Articles
by
E.P. Setyowati |
Total Records (
2 ) for
E.P. Setyowati |
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A. Isnansetyo
,
Trijoko
,
E.P. Setyowati
and
H.H. Anshory
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In this study, the extract was tested for in vitro
activity against oxytetracycline-resistant V. harveyi. Toxicity
of the methanolic extract was evaluated by Brain Shrimp Lethality test.
Geodia sp. was characterized by three spicula types (oxeas, trianes
and oxyaster euaster), encrusting growth formation, hispid surface features
and skeletal structure of paratangential ectosome. The methanolic extract
of Geodia sp. exhibited anti-oxytetracycline-resistant V. harveyi
activity with MIC of 31.25 μg mL-1. The extract also was
able to inhibit the growth of oxytetracycline-resistant V. harveyi
in broth culture at concentrations of 1 and 2xMICs and able to kill almost
V. harveyi cells at 4xMIC. Interestingly, the extract did not show
any toxic effect in Artemia salina up to 125 μg mL-1.
It is the first report for the antibacterial activity of methanolic extract
of Geodia sp. against oxytetracycline-resistant V. harveyi,
a pathogenic bacterium in marine aquaculture. This results suggest that
Geodia sp. might be used as a source of alternative compound to
control marine bacterial pathogen especially oxytetracycline-resistant
V. harveyi. |
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Sutomo
,
S. Wahyuono
,
S. Rianto
and
E.P. Setyowati
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A research had been conducted to isolated, to identify and to test biological activity of active isolates from n-hexane extracts of casturi (Mangifera casturi Konsterm.) fruit. The research aims at isolating active isolates from n-hexane fractions and testing for antioxidant and immunomodulatory activities. n-hexane fractions were isolated using column chromatographic method by using n-hexane-ethylacetate as eluents with eluent gradient (20:1; 15:1; 10:1; 8:2; 7:3; 6:4 and 5:5) v/v. The isolated compounds (purified isolates) were white crystals identified as terpenoid compound. Identification of chemical structures found a compound, namely, 1-isopropenyl-3a, 5a, 5b, 8, 8, 11a-hexamethyl-eicosahydro-cyclo penta [α]chrysen-9-ol (lupeol). The isolated compound was tested for antioxidant activity using DPPH (1,1-diphenyl -2-picrylhydrazyl) method with an IC50 value of 14384.71-lower than that of quercetin control (IC50 = 2.962). The finding shows that the isolate is not a potent compound against DPPH free radical inhibition. In vitro immunomodulatory activity test was conducted for the isolated compound. The isolates were given at dosages of 6.25, 12.5, 25, 50 and 100 μg. The test revealed increasing macrophage activity against latex bead phagocytosis. The highest phagocytosis activity and capacity were found at a dosage of 100 μg, namely, latex-bead consuming macrophage by 26% and macrophage-eaten latex bead by 32% (significant, compared to the negative control, namely, latex-bead eating macrophage by 13% and macrophage-eaten latex bead by 15%). |
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