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Articles by E.H. Boudyach
Total Records ( 2 ) for E.H. Boudyach
  H. Boubaker , B. Saadi , E.H. Boudyach and A. Ait Benaoumar
  In response to growers report of reduced efficacy of benzimidazole fungicides for control of cigar end-rot, a survey was conducted from 2002 to 2005 in four banana-growing locations, with various histories of benzimidazole use, in the Souss-Massa-Drâa Valley, Agadir to determine the proportion of isolates that were in vitro resistant to benomyl and thiophanate-methyl. Of the 274 isolates, collected in Biougra, Belfâa and Ouled-teima locations with more than 10 years of benzimidazole use, 65% (180/274) were resistant to benomyl, 67% (184/274) were resistant to thiophanate-methyl and 65% were resistant to both fungicides tested at a discriminatory concentration of 10 μg mL-1. Only 1.5% of isolates exhibited a differential reaction to the two fungicides. No resistance to benomyl and thiophanate-methyl were detected in isolates collected from banana plantations in the Tamri location which has no known history of benzimidazole use. The mean effective concentrations that reduced growth by 50% (EC50) for resistant-isolates of V. theobromae were between 80 and 97 μg mL-1 for benomyl and between 194 to 233 μg mL-1 for thiophanate-methyl. In contrast, wild-type isolates exhibited mean EC50 values for benomyl and thiophanate-methyl of 0.47 and 0.91 μg mL-1, respectively. All 305 isolates from the four locations sampled in this study were sensitive in vitro to chlorothalonil at 10 μg mL-1. Conidial germination of sensitive-isolates collected from banana plantations never exposed to benzimidazole fungicides were completely inhibited by 1000 μg mL-1 of benomyl or thiophanate-methyl. However, conidial germination of resistant-isolates was not affected by both fungicides tested at 1000 μg mL-1.
  H. Boubaker , B. Saadi , E.H. Boudyach and A.A. Benaoumar
  Green and blue molds (caused by Penicillium digitatum and P. italicum, respectively) are the main postharvest diseases of citrus fruits in Morocco. Following packing houses reports of reduced efficacy of fungicides used to control theses diseases, a survey was conducted during 2005-2006 packing season to characterize, both qualitatively and quantitatively, the resistance of P. digitatum and P. italicum to imazalil (IMZ) and thiabendazole (TBZ). Isolates of P. digitatum and P. italicum were obtained from decayed citrus fruits collected from commercial citrus packing houses located in the Souss-Massa-Draa (SMD), South of Morocco and were evaluated in-vitro for their sensitivity to IMZ and TBZ. Of the 290 P. digitatum isolates, 19% (55/290) were resistant to IMZ and 37% (107/290) were resistant to TBZ tested at discriminatory concentrations of 0.1 or 20 μg mL-1, respectively. In contrast, only 2.5 (5/204) and 21% (44/204) of P. italicum isolates collected from packing houses were resistant to IMZ and TBZ, respectively. No resistance to TBZ and IMZ were detected in Penicillium sp., isolates collected from a citrus orchard which has no known history of fungicide use. The proportion of collected isolates that were resistant to both fungicides was 1.5% for P. italicum and 10% for P. digitatum. The mean EC50 values for in vitro inhibition of mycelial growth of the P. digitatum resistant-isolates were between 0.81 and 0.98 μg mL-1 for IMZ, whereas those of TBZ were between 39.23 and 50.84 μg mL-1. The mean EC50 values for P. italicum resistant-isolates ranged from 0.53 to 0.61 μg mL-1 for IMZ and from 52.97 to 59.92 μg mL-1 for TBZ, whereas the mean EC50 values for orchard collected isolates were 0.04 μg mL-1 for IMZ and 0.16 μg mL-1 for TBZ. The data will provide a baseline for monitoring resistance to IMZ and TBZ in populations of P. digitatum and P. italicum in the SMD commercial citrus packing houses in the future.
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