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Articles by E. Mohammadi Goltapeh
Total Records ( 4 ) for E. Mohammadi Goltapeh
  Y.R. Danesh , E. Mohammadi Goltapeh , A. Alizadeh and M. Modarres Sanavy
  This research describes the optimized method of carrot hairy roots production for monoxenic culture of Arbuscular Mycorrhizal Fungi (AMF) in Iran. Inoculation of mature carrot root sections was carried out using four isolates of Agrobacterium rhizogenes as well as a mixture of isolates that had been grown in three culture media (LB, YMA and modified medium) with different incubation times (24 and 36 h) before inoculation. Three methods of carrot hairy root initiation were compared in an attempt to optimize hairy root production in terms of the number of root initiations and the health (lack of rotting) of the explants. Results showed that most treatments resulted in hairy root formation, although not equally so. It was shown that the mixture of bacterial strains from three culture media increased the frequency of bacterial colonies with a good transformation characteristic. Also, incubation of inoculated carrot discs for 48-72h in darkness provided a suitable condition for bacterial strains to insert their copies of Ri T-DNA. The source of the carrots was one of the most important factors. With mature, freshly harvested carrots showing better hairy root production. Dissection of the cortex to expose the cambium had a positive effect on hairy root initiation. Increasing bacterial strains incubation time from 24 to 36 h improved transformation efficiency in all treatments but not in method III. Results showed that the best method for initiation of hairy root induction in carrots especially in Iran is the method I.
  K. Kheradmand , K. Kamali , Y. Fathipour , M. Barzegar and E. Mohammadi Goltapeh
  The present study indicated that the pigmy mite Pediculaster fletchmenni (Acari: Siteroptidae) can cause qualitative losses by influencing on nutritional values of the button mushroom Agaricus bisporous. A comparison of dry matter (DM), organic matter (OM) and mineral elements was made by releasing various density of mites consisted of 0, 50, 100 and 250 adult mites per kg of compost. Mineral elements including P, Ca, Mg, Na, Fe, Zn, Cu and Mn were determined by ICP atomic spectroscopy. The samples organic matter varied from 90-93% and the DM contents ranged between 13 to 14%. The ranges of variations for these minerals in control and other treatments were 1230.4- 880.2, 208.3- 66.6, 153.9- 91.1, 154.6- 60.4, 23.4- 5.6, 4.5- 3.4, 3.8-1.3 and 0.911-0.480 (mg/100 g dw), respectively. The amounts of Ca, Cu, Fe, K, Mg, Mn, Na, Zn and P were decreased at highest density of mites, 250 mites per kg of compost, percent of decrease for the above elements are: 61.3, 48.9, 67.2, 12.8, 21.3, 45.1, 27.6, 21.0 and 2.7%, respectively.
  R. Heydari , E. Pourjam and E. Mohammadi Goltapeh
  Antagonistic effect of five species of Pleurotus, including P. ostreatus, P. sajor-caju, P. cornucopiae, P. florida and P. eryngii on second stage juveniles (J2) of Meloidogyne javanica were studied, in vitro. On water agar, all of the species tested produced tiny droplets of toxin. Nematodes touching such droplets showed a sudden response and became colonized by the fungi after 24-48 h. Filtrates of the tested fungi grown in malt extract broth were toxic on the nematodes but this toxicity varied between species. Culture filtrates of P. ostreatus showed the highest nematicidal activity toward M. javanica J2 and the lowest toxic effect was observed in filtrates of P. eryngii. A linear relationship was proved between increasing toxin concentration and the percentage of dead nematodes.
  J. Abdollahzadeh , E. Mohammadi Goltapeh and H. Rouhani
  Based on in vitro results, three isolates: T. harzianum (J1), T. harzianum (J6) and T. virens were selected for greenhouse studies. Biocontrol efficacy of these isolates as well as the combination of T. harzianum (J1) and T. virens on crown and root rot of sunflower were studied in series of experiments using three methods; incorporation of conidial suspension into the soil (107 conidia g-1 soil), incorporation of conidial suspension mixed with compost (107 conidia g-1 compost) into the soil and impregnating of sunflower seeds with conidial suspension (107 conidia mL-1). Experimental data were analyzed with Randomized Complete Block Design (RCBD) and means compared by Duncan`s multiple range test. Greenhouse experiments (in vivo) showed that, the combination of T. harzianum (J1) and T. virens using incorporation of conidial suspension mixed with compost into the soil had the most efficiency on biocontrol of S. minor and reduced disease incidence up to 50%.
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