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Articles by Dwi Sutiningsih
Total Records ( 5 ) for Dwi Sutiningsih
  Dwi Sutiningsih , Mohamad Arie Wuryanto , Henry Setyawan Susanto , Sujud Hariyadi and Mustofa
  Background and Objective: Linamarin is an active compound isolated from the leaves of cassava (Manihot esculenta Cranz) that has cytotoxic effects on HT-29, MCF-7 and HL-60 cells. This study was aimed to determine the cytotoxic and antiproliferation activity and induction of p53 protein in Raji cells after administration of various concentrations of linamarin from cassava leaves (Manihot esculenta Cranz). Materials and Methods: Linamarin was isolated from cassava leaves (Manihot esculenta Cranz) using a multilevel purification method. Linamarin cytotoxicity was tested on Raji cells using the MTT method, while antiproliferation activity was tested using a doubling time test. The p53 protein expression was observed by immunocytochemical tests. The cytotoxic activity of Raji cells was expressed by the value of Inhibitory concentration 50 μg mL1. The doubling time was calculated by comparing the slope values of the log graphs of the number of cells at various times. Raji cells that were positive for p53 protein showed brown painted nuclei or cytoplasm. Results: Linamarin from cassava leaves can inhibit cytotoxic activity and proliferation on Raji cells. The higher the linamarin concentration, the longer the doubling time of Raji cells. The expression of p53 protein on Raji cells after linamarin administration was higher than the control. The p53 protein expression was found in the nuclei (91.05%) and cytoplasm (8.95%). Conclusion: Based on those findings, linamarin from cassava leaves has the potential to be developed as an anticancer agent.
  Dwi Sutiningsih and Nurjazuli
  Background and Objective: Brusatol is one of quassinoid, derived from Makassar fruit (Brucea javanica (L.) Merr.) and belong to the family of Simaroubaceae. Brusatol has been proven to have insecticidal and anti-feeding activity on larvae of Spodoptera exigua. This study aimed to investigate the larvicidal activity and behavioral response to the larvae of A. aegypti after administration of brusatol and to demonstrate cytotoxicity activity in vero cells. Materials and Methods: Larvicidal activity was monitored using bioassay test. The behavioral response of larval A. aegypti was observed after treatment with various brusatol concentrations and compared directly with control every 10, 30 min and 1, 5 and 24 h. The examination of brusatol cytotoxicity on vero cells performed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) method. Results: The results of this study showed that the behavioral response of A. aegypti larvae after treatment with brusatol has similarity with the behavioral response after treatment with temephos 1 ppm. The behavioral response of A. aegypti larvae showed symptoms of a nerve poison that is excitation, convulsions, tremors and paralysis. The lethal concentration of 50 and 90 (LC50, LC90) to A. aegypti larvae were 0.669±0.106 and 8.331±0.060 ppm, respectively. Brusatol cytotoxic activity on vero cells is low, with IC50 values worth 1402.029±0.164 mg mL–1. Conclusion: The results of this study proved the potential of brusatol as biolarvicide to control the mosquito vector with low levels of cytotoxicity.
  Dwi Sutiningsih , Mustofa , Tri Baskoro Tunggul Satoto and Edhi Martono
  Background and Objective: Bruceine A is a quassinoid compound isolated from the seeds of Makassar fruit (Brucea javanica L. Merr.) that has a biolarvicidal effect toward Aedes aegypti larvae with a lethal concentration fifty (LC50) and ninety (LC90) of 0.408 and 4.923 ppm, respectively. This study aimed to demonstrate the inhibitory effect of bruceine A on the growth and development of A. aegypti larvae at sublethal concentrations. Materials and Methods: The effect of bruceine A on A. aegypti larvae growth and development was tested using bioassays. Daily counts of the number of larvae that survived or died were taken until all the experimental larvae became either pupae or adults. Dead larvae were assessed for morphological and histological changes. The statistical analysis of growth and development inhibition was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on morphological and histological changes were descriptively analyzed. Results: Inhibitory effects of bruceine A on the growth and development of A. aegypti larvae were seen at concentrations as low as 0.01 ppm. The morphological changes seen in larvae included damage to the gastrointestinal tract manifested as black spots, folded breathing tubes and detached cuticle and setae as well as smaller body sizes compared to the control group. Histological examination of the larvae indicated diffuse necrosis in the gastrointestinal epithelium accompanied by epithelial cell shrinkage, whereas the core was not visible. Conclusion: It is concluded that bruceine A inhibits growth and development of A. aegypti larvae, as shown by cuticle damage and necrosis of gastrointestinal and respiratory epithelial cells.
  Aris Sugiharto , Suharyo Hadisaputro , Soegiyanto , Hastaning Sakti , Hardhono Susanto , Tandiyo Rahayu , Ari Suwondo and Dwi Sutiningsih
  Background and Objective: Elderly fitness should not only improve by physical activities alone, but improvements are generally better if done through teaching physical and psychological activities based on mental and physical unity. This study was aimed to assess the influence of the fitness-empowering elderly intervention programs on the level of fitness of the elderly. Materials and Methods: The test was conducted on 60 respondents who were divided into 3 groups, group 1 underwent elderly calisthenics and interactive counseling skills, group 2 elderly calisthenics and group 3 was a control group. The independent variables were calisthenics for elderly and interactive counseling skills, the dependent variables VO2Max, muscle flexibility, resting pulse, self-efficacy, health commitment. Data were analyzed by testing differences between groups, using the ANOVA test followed by the post hoc Tukey test and by the Kruskal-Wallis test followed by the Mann-Whitney test. Results: There was a significant difference in mean measurement results between before and after the intervention of the fitness empowerment elderly program in the form of elderly calisthenics and interactive counseling skills after 6 weeks with a frequency of 3 times per week including VO2Max, muscle flexibility, self-efficacy and health commitment. After the intervention can improve the measurement results between groups. Conclusion: The fitness empowerment elderly program can increase VO2Max, muscle flexibility, self efficacy and health commitment.
  Dwi Sutiningsih , Mustofa , Tri Baskoro Tunggul Satoto and Edhi Martono
  Background and Objectives: Aedes aegypti Linnaeus is a mosquito species that plays an important role as a vector of diseases in tropical and subtropical regions. Aedes aegypti (L.) resistance against temephos and pyrethroid has been widely reported in several countries including Indonesia. This study aimed to examine the neurotoxic mechanism of bruceine A biolarvicide and to determine the inhibitory activity of the enzyme acetylcholinesterase and gene Voltage-Gated Sodium Channel (VGSC) on A. aegypti (L.) larvae. Materials and Methods: Bruceine A was obtained by extraction and isolation of Makassar fruit’s seeds. Test of inhibition of acetylcholinesterase activity was based on Ellman method. Voltage-Gated Sodium Channel gene inhibitor was tested using PCR. The statistical analysis of inhibition of the enzyme acetylcholinesterase was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on behavior response and VGSC gene were descriptively analyzed. Results: Bruceine A could inhibit the action of the enzyme acetylcholinesterase as much as 45.083±0.003%, with symptoms of a neurotoxic that is excitation, convulsions, tremors and paralysis. The result of fragment amplification of VGSC gene and electrophoresis on agarose gel 2% generated in a specific band with the size of the fragment bands of 336 bp on samples of larvae that were still sensitive to bruceine A and cypermethrin. Conclusion: Bruceine A isolated from the seeds of Brucea javanica (L.) Merr. has been demonstrated as biolarvicide against larvae of A. aegypti (L.), through its neurotoxic properties. The mechanism of neurotoxicity is mediated through inhibition of enzyme acetylcholinesterase and VGSC gene.
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