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Articles by Duoyao Cao
Total Records ( 3 ) for Duoyao Cao
  Duoyao Cao , Xiaoqi Jing , Xiaoyu Wang , Hui Liu and Dekun Chen
  Staphylococcus aureus is one of the major pathogen which can cause mastitis in dairy animal. CD4+ lymphocytes play a significant role in clearing this type of bacteria. To our knowledge, CD4+ lymphocytes change in mouse model of S. aureus mastitis is poorly understood. To investigate the response of CD4+ lymphocytes in mammary gland, the mice mastitis was induced with S. aureus in this study. New protocol was used to separate lymphocytes in mammary gland. The results showed that CD4+ lymphocytes increased significantly comparing with blank and saline mice (p = 0.0019, p<0.01) at 72 h post Intramammary Inoculation (IMI). At the same time, the infection mice mammary gland tissues were swelled and were suffering from hyperemia. From histological observation, massive inflammatory cells are infiltrated in the tissue. The results suggest that CD4+ lymphocyte is one of important cells in against S. aureus infection in mammary gland.
  Xiaoqi Jing , Duoyao Cao , Hui Liu , Xiaoyu Wang , Xuanduo Zhao and Dekun Chen
  Staphylococcus aureus is a contagious, mastitis-causing pathogen that is highly adapted to survive in mammary gland. This study focuses on γδ T cell populations in mammary gland involved in the immune response against S. aureus. The C57BL/6J mice in 10-15th day of lactation were intramammary challenge with S. aureus isolated from goat chronic mastitis or with PBS (control). γδ T1(interferon (IFN)-γ-producing) cells and γδ T17 (interleukin (IL)-17-producing) cells in the mammary gland during infection were evaluated by cytometry. The results showed that the percentage of γδ T1 in γδ T cells decreased following intramammary injection and significance was observed at 8, 36, 48, 60 and 72 h compared with control. At the same time, expression of IFN-γ mRNA was decreased. However, percentage of γδ T17 in γδ T cells was increased significantly at 18, 48 and 60 h post challenge, accompanied by an increase in the expression folds of IL-17, IL-23p19 and CCR6 mRNA following intramammary injection. Overall, these data suggest that γδ T17 cells, not γδ T1 cells, in mammary gland tissue were activated rapidly and played an important role in mediating mammary inflammation caused by S. aureus.
  Xiaoqi Jing , Yansen Han , Duoyao Cao , Shan Mou , Hui Liu , Jing Yao , Lijuan Zhao , Yanqing Zhao , Chuanchang Shang and Dekun Chen
  IL-17 is a crucial mediator of mucosal inflammation for extracellular bacteria clearance. The production of IL-17 was associated with TGF-β and multiple proinflammatary cytokines, especially IL-6 and IL-1β. IL-17 can cooperate additively or synergistically with IL-6 or IL-1β to amplify of inflammatory processes. However, the roles of IL-17 and cytokines associated with IL-17 in dairy goat mastitis is poorly understood. In the present study, the concentrations of IL-17, IL-6, IL-1β and TGF-β were assayed in sera and milk of Guanzhong dairy goat mastitis induced with Escherichia coli using the Enzyme-Linked Immunosorbent Assay (ELISA) at several time points before and after challenge. The results showed that the levels of all cytokines varied slightly in sera while raised significantly in milk post intramammary inoculation with E. coli. The results suggest that IL-17 is one of important mediators in mammary gland inflammation for bacterial clearance in dairy goat mastitis and IL-6 and TGF-β play an important role or help for development in IL-17 producing cells in goat.
 
 
 
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