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Articles by Doru PAMFIL
Total Records ( 10 ) for Doru PAMFIL
  Rodica POP , Doru PAMFIL , Monica HARTA , Ioan HAS and Iulia POP
  Genetic analysis with RAPD markers has been extensively used to determine diversity among maize genotypes. The aim of the present study was to estimate genetic relationships among 70 genotypes, provided from SCDA Turda Cluj germplasm collection. RAPD analysis was performed with 14 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 13 bands (OPA 04) and 7 bands (OPAL 20). The highest numbers of polymorphic bands were obtained with primer OPA 04, respectively 13 bands, following by OPO 12 (12 polymorphic bands), OPAB 11 and OPA 17 (11 polymorphic bands). Lowest number was obtained with primer OPAL 20, respectively 7 polymorphic bands. Genetic distances were established using Nei-Li coefficient and UPGMA dendrogram was constructed with RAPDistance 1.04 software. The built dendrogram shows phylogenetic relationships between genotypes analyzed.
  Ionut GOTEA , Doru PAMFIL and Rodica GOTEA
  Not available
  Ionut GOTEA , Doru PAMFIL and Rodica GOTEA
  Not available
  Rodica POP , Ioan HAS , Iulia Francesca POP , Monica HARTA and Doru PAMFIL
  Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a breeding program. Our objective was to investigate the level of genetic diversity among maize inbred lines. Eighty-three maize inbred lines obtained from SCDA Turda were genotyped using 20 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 17 bands (OPA 03) and 7 bands (OPAB 11). The highest numbers of polymorphic bands were obtained with primer OPA 03, respectively 17 bands, followed by OPA 01, OPB 08 (16 polymorphic bands) and OPX 03 and OPAL 20 (13 polymorphic bands). Genetic distances were established using Nei Li/Dice coefficent and an UPGMA dendrogram was constructed with FreeTree software. The built dendrogram shows phylogenetic relationships between the analysed biological material.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL , Erika BALAZS and Constantin BOTEZ
  Not available
  Adriana SESTRAS , Radu SESTRAS , Doru PAMFIL and Adrian BARBOS
  Resistance to diseases and pests remain important characteristics of interest for pear breeders. Among pear’s diseases, pear leaf spot, caused by the fungus Mycosphaerella pyri, occurs in Transylvania conditions, Romania, and could produce yield decreases and negative economic effects. At FRS Station Cluj-Napoca, the segregation for pear leaf spot response to attack of F1 pear hybrids belonging to six combinations was analysed. There were studied the seedlings originated in incomplete diallel cross, effectuated with four genitors (Comtesse de Paris, Milenium, Virgiliu Hibernal, Cluj 89-1-81 selection) by direct hybridization, without reciprocal and self-pollination. The seedlings were framed in six classes of their response to leaf spot attack, during two consecutive years, in absence of fungicide treatments, using a scale of notation from 1 (no attack) to 5 (very severe attack). The resistance/susceptibility of seedlings to pear leaf spot attack was considered as quantitative parameters and was computed by Griffing, respectively Masiukova model. The result for hybrids’ response to disease, appreciated by marks on hybrid and mean of marks in each hybrids combination, revealed marked differences among families, depending on genitors. General and specific combining ability (GCA and SCA) suggested that the pear response to leaf spot disease is under additive genes control, and, because their GCA values, cultivars like Comptesse de Paris and Milenium can transmit to its progenies tolerance or even resistance to disease. Based on the values of GCA and SCA, selection of genitors can be efficient for desirable characters like their response to pear leaf spot disease attack and capacity to transmit the resistance to their progenies.
  Alexandru C. BRICIU , Doru PAMFIL , Ioan ZAGRAI , Daniela BRICIU , Ioana PETRICELE , Dana CURTICIU and Abdelmoumen TAOUTAOU
  Plum pox virus (PPV) is the causal agent of sharka disease, which is responsible for severe damage and important economic losses in the stone fruit industry. The disease mainly affects stone-fruit species, mainly apricot, plum and peach. Sharka is originated from Eastern Europe and was described for the first time around 1915 in Bulgaria (Atanasoff, 1932). In this study we collected thirty PPV isolates from one experimental orchard belonging to the Fruit Research and Development Station Cluj. Molecular strain differentiation was done with the help of RT-PCR technique by analyzing the genomic region (Cter)CP of the virus by using RFLP analysis we were able to distinguish the two major strains, D and M, based on Rsa I polymorphism located in the genomic region (Cter) CP.
  Daniela BRICIU , Doru PAMFIL , Alexandru BRICIU , Dana CURTICIU , Erika BALAZS , Abdelmoumen TAOUTAOU , Iulia POP and Laura COTA
  The polymorphic study of table grapes was performed on ten varieties provided from two resorts grapevine: S.D.E. Cluj and S.D.E. Iasi. To achieve this study on grape varieties we used RAPD method. Genetic diversity and relationships between individuals was evaluation on the basis of presence or absence of bands resulting specific genetic fingerprinting. In this purpose we tested a total of 22 RAPD primers only 12 were polymorph. We made two rehearsals for each primer, taking into account only visible bands presents in the two rehearsals. The fragments resulted by amplification with polymorph primers have a lengths between 200 and 1500 bp. Notation of bands has been made by reference to DNA marker with 1 the presence of bands and with 0 the absence of bands. On the basis of these results dendrogram was constructed.
  Daniela BRICIU , Doru PAMFIL , Alexandru BRICIU , Dana CURTICIU , Erika BALAZS , Abdelmoumen TAOUTAOU , Iulia POP and Laura COTA
  The wine sector is one of the economically most important agricultural activities in the world. An enormous diversity of Vitis vinifera L. varieties (cultivars) can be used in the production of wine, although only a small number is of commercial importance. Ten grapevine varieties have been analysed. Five varieties are used for red wine production and five for white wine production. Two types of samples were analyzed: young leaves and must. Leaves were collected at the S.D.E Cluj. Must was obtained right after grape crushing, containing the skin and the solid parts of the flesh. Three different methods were tested: Lodhi et al., (1994) modified by Pop et al., (2003); Faria et al., (2000); Sambrook et al., (1989). The first method was used for DNA extraction from leaves and also from must. Analyses with DNA extracted from leaves and monovarietal musts were performed using the following microsatellite loci: VVS2, VVS5, VVMD5, VVMD7, ssVrZAG 47, ssVrZAG 62 and ssVrZAG79. In DNA extraction methods we taken into account the fact that PCR is very sensitive to the presence of Taq polymerase inhibitors. Results showed that for must samples the second method was successful. In order to see if the allelic profile from the must is identical to the one from leaves it was charged in the same polyacrylamide gel PCR product from must and leaves. The size of microsatellite alleles obtained in must is in accordance with those from leaves. Extraction of DNA from leaves and must was achieved yielding SSR amplification. SSR markers could distinguish all varieties used in this study.
  Liviu A. VESCAN , Doru PAMFIL , Ioan ZAGRAI , Ioana V. BERINDEAN , Doina CLAPA , Oana CIUZAN and Iulia F. POP
  Sharka, caused by Plum Pox Virus, is an important disease that greatly affects several stone fruits species. In vitro culture was started from PPV positively confirmed Romanian plum cultivars Delia and Tita, using meristems from apical and axillary buds. Six multiplication subcultures of the apical shoots were undertaken. Tita proved to be more responsive to proliferation with a maximum of 22 shoots per initial explant, when using MS media supplemented with BAP 0.5 mg/l. The plantlets were subjected to molecular analyzes using RT-PCR after the third and sixth subculture in order to establish the infection rate. No more than 31% virus free plantlets were obtained in average for the two cultivars using micropropagation alone. As an alternative, in vitro thermo-, chemo- and thermo-chemotherapy techniques were tested. For the chemotherapy treatment, a maximum survival rate of 75 and 45.8% was obtained when using concentrations of 50 and 100 mg/l ribavirin. Ribavirin inhibited the growth of the plantlets even in the second subculture, when absent. Further molecular analyses of the chemotherapy-derived plantlets will be carried out. For thermotherapy the conditions were set to 36°C, 80% humidity and 16 hours photoperiod. Thermotherapy and thermo-chemotherapy resulted in total necroses of the plantlets after the fourth week, regardless the cultivar or basal media used. PPV elimination was momentary successfully achieved for several in vitro plantlets only when using simple micropropagation techniques.
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