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Articles by Diane Ordway
Total Records ( 3 ) for Diane Ordway
  Diane Ordway , Marcela Henao-Tamayo , Crystal Shanley , Erin E. Smith , Gopinath Palanisamy , Baolin Wang , Randall J. Basaraba and Ian M. Orme
  Mycobacterium bovis bacillus Calmette-Guérin (BCG) currently remains the only licensed vaccine for the prevention of tuberculosis. In this study, we used a newly described flow cytometric technique to monitor changes in cell populations accumulating in the lungs and lymph nodes of naïve and vaccinated guinea pigs challenged by low-dose aerosol infection with virulent Mycobacterium tuberculosis. As anticipated, vaccinated guinea pigs controlled the growth of the challenge infection more efficiently than controls did. This early phase of bacterial control in immune animals was associated with increased accumulation of CD4 and CD8 T cells, including cells expressing the activation marker CD45, as well as macrophages expressing class II major histocompatibility complex molecules. As the infection continued, the numbers of T cells in the lungs of vaccinated animals waned, whereas the numbers of these cells expressing CD45 increased. Whereas BCG vaccination reduced the influx of heterophils (neutrophils) into the lungs, an early B-cell influx was observed in these vaccinated animals. Overall, vaccine protection was associated with reduced pathology and lung damage in the vaccinated animals. These data provide the first direct evidence that BCG vaccination accelerates the influx of protective T-cell and macrophage populations into the infected lungs, diminishes the accumulation of nonprotective cell populations, and reduces the severity of lung pathology.
  Marta Martins , Miguel Viveiros , Diane Ordway , Jette E. Kristiansen , Joseph Molnar and Leonard Amaral
  Killing of bacteria by neutrophils is dependent upon the availability of potassium. Although macrophages derived from human peripheral blood monocytes have little killing activity of their own, they can be transformed into effective killers of Staphylococcus aureus and Mycobacterium tuberculosis by in vitro exposure of the macrophage to clinically relevant concentrations of phenohiazines, namely, thioridazine or chlorpromazine. Because transport mechanisms dependent upon the availability of calcium are inhibited by these agents, the possibility that other agents which have similar activity also have the ability to enhance the killing of bacteria by the macrophage derived from peripheral blood monocytes was investigated. In this study we show that the presence of increasing concentrations of ouabain, reserpine or verapamil in the medium enhances the killing of Staphylococcus aureus. Because these concentrations have no activity on the replication or killing of the bacterium, killing is deemed to be due to the macrophage itself. A model is presented which describes the mechanism by which these agents and phenothiazines indirectly activate lysosomal enzymes as a result of the inhibition of potassium efflux pumps that would normally pump the ion from the phagocytic vacuole to the cytoplasm of the macrophage.
  Marta Martins , Miguel Viveiros , Diane Ordway , Jette E. Kristiansen , Joseph Molnar and Leonard Amaral
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