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Articles by Dhia S. Hassawi
Total Records ( 4 ) for Dhia S. Hassawi
  Ahmad F. Hussain , Ghandi H. Anfoka and Dhia S. Hassawi
  In this study, different tomato genotypes were transformed with silencing trigger construct that showed high efficiency in controlling Tomato yellow leaf curl virus. Cotyledons and hypocotyls of the tomato hybrid (981 X TY-6) were cultured on eight regeneration media containing different concentrations of auxins and cytokinins. Zeatin and Indole Acetic Acid (IAA) at 1 and 0.1 mg L-1, respectively, were significantly enhanced shoot regeneration. Different concentrations of Phosphinothericin (PPT) were used to select transformed tomato plantlets; the lowest PPT concentration (5 mg L-1) inhibited the growth of non-transformed plantlets but did not affect the growth of transformed plantlets. The inhibitory effects of four antibiotics (carbincillin, clavamox, cefotaxin and kanamycin) on the growth of Agrobacterium tumefaciens and the regeneration of shoots were investigated; carbincillin and clavamox at 500 mg L-1 showed high inhibitory effect. The transformation data indicated that the rate of regeneration from transformed tomato line (MP-1) was significantly higher than that of tomato hybrid (981 X TY-6).
  Azmi D. Hawari and Dhia S. Hassawi
  This study was conducted to establish data on mastitis in she-camels in Jordan. Milk samples were collected from 90 dromedary camels in south province of Jordan. California Mastitis Test (CMT) gave results with 70 milk samples; 42 samples (60%) showed positive CMT. Infection with some bacterial species was associated with positive CMT. About 21% of the camels revealed clinical signs of mastitis. The highest percentage of bacterial count, which range from 3.0x102 to <3.0x103 cfu mL-1, was founded in the milk samples. The most predominant bacterial isolates were Micrococcus spp., Staphylococcus aureus, Streptococcus spp. and Corynebacterium spp. Gentamycin, Ampicillin and Tetracycline were the most effective antimicrobial agents against the bacterial isolates.
  Amal A. Al-Abbadi , Ihab Ghabeish and Dhia S. Hassawi
  Little information exists in Jordan about the presence of viral diseases in honey bee (Apis mellifera) colonies and their association with Varroa destructor mites. The first investigation using molecular techniques of honey bees colonies infested with Varroa mites for the presence of three viruses in Jordan is reported. Samples of adult bees, larvae and Varroa mites were collected from twenty four colonies represented seven different locations of Jordan. Infestation percentages were 54.2% for Israeli Acute Paralysis Virus (IAPV) followed by 11.1% for Deformed Wing Virus (DWV) and 4.2% for Black Queen Cell Virus (BQCV). IAPV and DWV were detected in adult bees, larvae and Varroa, while, BQCV was found only in adult bees and Varroa. The results were confirmed by analyzing the samples with reverse transcription-PCR (RT-PCR) for the three viruses surveyed using specific primers. This is the first molecular detection of these three viruses infecting honey bees in Jordan.
  Sahar S. Hanania , Dhia S. Hassawi and Nidal M. Irshaid
  To determine the phenotypic, allelic frequencies and the genotypes of ABO blood groups in a Jordanian population, samples of 12215 randomly healthy Jordanian voluntary blood donors during the period 1998-2003 were taken from the National Blood Bank donor registry, Amman, Jordan. The results of the phenotypic distribution indicated that 4686 (38.36%) of the donors were type A, 4473 (36.62%) O, 2203 (18.04%) B and 853 (6.98%) AB. The gene frequencies were 0.6052 for Io allele, 0.2607 for Ia allele and 0.1341 for Ib allele. Using PCR-RFLP technique, two separate segments of the transferase gene containing nucleotide 261 in exon 6 and nucleotide 703 in exon 7 of the ABO gene locus were amplified and their products were analyzed with two restriction enzymes (KpnI and AluI). The electrophoresis patterns of 105 samples showed that ABO genotypes were AA: 6 (5.714%), AO: 35 (33.333%), BB: 1 (0.953%), BO: 14 (13.333%), AB: 10 (9.524%) and OO: 39 (37.143%).
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