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Articles by Daryoush Mohajeri
Total Records ( 9 ) for Daryoush Mohajeri
  Daryoush Mohajeri , Ghafour Mousavi , Mehran Mesgari , Yousef Doustar and Mir Hadi Khayat Nouri
  The medicinal properties attributed to Crocus sativus L. (saffron) are extensive. The safety of saffron is important in relation to its medicinal applications. This study was performed to elucidate the possible toxic effects of ethanolic extract of Crocus sativus L. stigma on liver, kidney and some hematological parameters in rats. Wistar rats were randomly assigned into four groups of eight animals each. Group 1 was treated with ISS as control and Groups 2 to 4 were treated with extract administered daily for 2 weeks intraperitoneally in doses of 0.35, 0.70 and 1.05 g kg-1, respectively. Body weight of the animals were recorded on the first, seven and final days of the experiment. The haematological studies include total RBC count, total WBC count, Hb, %HCT, MCV, MCH and MCHC. Biochemical/serum profile studies include ALT, AST, urea, uric acid and creatinine. Tissue specimens of the liver and kidneys were subjected to histological examination using standard hematoxyline-eosin staining. The extract caused significant reductions in the Hb and HCT levels and total RBC count, although it showed any dose-dependent effect. Total WBC count showed significant dose-dependent increases in extract treated rats. Significant dose-dependent increased values of AST, ALT, urea, uric acid and creatinine were seen. Microscopically, there were mild to severe hepatic and renal tissue injuries supporting the biochemical analysis. The results indicated that extract of Crocus sativus L. stigma is toxic in high doses.
  Yousef Doustar , Daryoush Mohajeri , Younes Anzabi and Mehrdad Nazeri
  Influenza virus produces cell death in animals and human. Since cell death can be caused by either necrosis or apoptosis. We investigated the types of cell death that occur in chickens infected with avian influenza virus, A/chicken/Iran/772/2000(H9N2). In experimental study 60 SPF chickens at 3 weeks old were divided to two groups. The first group was infected with 107.5 EID50 titer of the virus intranasaly and the second group was treated with saline normal. Following 72 hrs, renal tissues were collected and fixed in 10% formalin solution. The prepared microscopic sections with the thickness of 5-6 micron were stained using TUNEL method. In comparison to the control group, there were significant mean difference of apoptotic cells in renal tubular cells of the infected group (p<0.005). We demonstrated that A/chicken/Iran/772/2000 (H9N2) is able to induce apoptosis in renal tubular cells.
  Yousef Doustar , Daryoush Mohajeri , Alireza Garjani , Ghafour Mousavi and Mehrdad Neshat Ghramaleki
  The heart failures following infarctions is one of the most important causes of death throughout the world. This study was undertaken to investigate the protective effects of metformin on apoptotic cell death of cardiomyocytes during experimental cardiac Ischemia-Reperfusion (IR) in rats. The 25 male Wistar rats were randomly assigned into 5 groups of 5 animals each including; Sham/IR, IR, low dose metformin+IR, average dose metformin+IR and high dose metformin+IR. Heart muscle ischemia was induced clamping the left descending coronary artery. After 30 min of ischemia, the clamps were taken off and the animals underwent 2 h reperfusion. Metformin (5, 10 and 20 μg/kg/min) was infused 15 min prior to reperfusion through jugular vein in treatment groups. At the end of experiment, the rats were euthanized and histological sections from left ventricles were prepared through Tunnel Staining method. Apoptotic cells were counted under light microscope. The data obtained were statistically analyzed using ANOVA. Differences were considered statistically significant at p<0.05. In group 2, ischemia-reperfusion caused occurrence of apoptotic cell death in cardiomyocytes. There was a significant increase in the incidence rate of apoptosis of cardiomyocytes in comparison with group 1 (p<0.001). In groups 3-5 metformin (5, 10 and 20 μg/kg/min) caused significant decrease in the number of apoptotic cells in comparison with group 2 (p<0.05, p<0.01 and p<0.001, respectively). This study therefore, suggests that metformin may be a useful agent for the prevention of Ischemia-Reperfusion (IR) induced apoptotic cell death of cardiomyocytes in a dose dependent manner in the rats.
  Mohammad Rahbani , Daryoush Mohajeri , Ali Rezaie and Mehrdad Nazeri
  The negative impact of diabetes on the retinal, renal, nervous and cardiovascular systems is well recognized yet little is known about the effect of this disease on the liver. Oxidative stress is currently suggested as a mechanism underlying diabetes mellitus complications. The present study was designed to assess the liver injury as a complication of diabetes mellitus and to evaluate the hepaoprotective properties of ethanolic extract of Saffron (dried stigmas of Crocus sativus L.) in streptozotocin-induced diabetes in rats. Aminotransferases, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) were measured to determine the concentration of intracellular hepatic enzymes that have leaked into the circulation and serve as a marker of hepatocyte injury. Alkaline Phosphatase (ALP) and bilirubin were measured to assess biliary function. Albumin was measured to reflect liver synthetic function. The lipid peroxidation product, Malondialdehyde (MDA) and reduced Glutathione (GSH) content was measured to assess free radical activity in the liver tissues. The enzymatic activities of Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured as indicators of antioxidation in liver tissue. Moreover, histopathological observations were assayed at the degree of hepatic injury. For this purpose, male Wistar rats were made diabetic with a single injection of STZ (75 mg kg-1 i.p.). Rats were randomly separated into four groups, each containing 10 animals: Group 1, healthy control rats; Group 2 non-diabetic rats were treated with 40 mg kg-1 b.w./day intraperitoneal (i.p.) injection of Saffron extract; Group 3, diabetic rats; Group 4, diabetic rats were treated with saffron extract (40 mg kg-1 b.w./day, i.p.) for a period of 8 weeks. At the end of the experiment, MDA contents of the liver tissue and serum levels of ALT, AST, AP and bilirubin in Groups 3 were found to be significantly increased as compared to Group 1 (p<0.05) and these serum biomarkers of hepatic injury and liver MDA level in Group 4 were significantly decreased as compared to Group 3 (p<0.05). The GSH, SOD, CAT and GSH-Px contents of the liver and serum albumin level in Group 3 was significantly decreased as compared to Group 1 (p<0.05) and were found to be significantly increased in Group 4 as compared to Groups 3 (p<0.05). Histopathological changes were in agreement with biochemical findings. Based on these findings, it is presumed that ethanolic extract of C. sativus L. stigma may have the hepatoprotective effect in experimentally induced diabetic rats. Researchers suggest that saffron extract has beneficial effects on antioxidant defence of diabetic liver tissue.
  Daryoush Mohajeri and Mehrdad Nazeri
  Non-Alcoholic Fatty Liver Disease (NAFLD) is now recognized as the most common type of liver disease and might lead to an important public health problem. The aim of the present study was to evaluate the protective effects of Crocin, a unique water-soluble glycosylated carotenoid in the stigmas of the Crocus sativus Linne and in the fruits of Gardenia jasminoides Ellis, on rat high fat diet-induced hepatic steatosis model. Male Wistar rats were given either control diet, high fat diet alone or high fat diet plus Crocin via gavage at different doses (25, 50 and 100 mg/kg/day) for 4 weeks in different experimental groups. The serum levels of Triglyceride (TG), Total Cholesterol (TC), Low-Density Lipoprotein (LDL-C), Very Low-Density Lipoprotein (VLDL-C) and High-Density Lipoprotein (HDL-C) were detected to determine deleterious metabolic effects. Aminotransferases, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) were measured to determine hepatocyte injury. Alkaline Phosphatase (ALP) and bilirubin were measured to assess biliary function. Albumin was measured to reflect liver synthetic function. The lipid peroxidation product, Malondialdehyde (MDA) and reduced Glutathione (GSH) content was measured to assess free radical activity in the liver tissues. The enzymatic activities of Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured as indicators of antioxidation in liver tissue. Finally, the biochemical findings were matched with histopathological verifications. After 4 weeks, high fat diet caused deleterious metabolic effects including hypertriglyceridemia, hypercholesterolemia and liver dysfunction. Rats fed high fat diet alone showed increased activities of hepatocellular enzymes in plasma, significant decline in antioxidants and elevated lipid peroxidation indices in liver. Crocin treatment significantly reduced elevated markers of liver tissue injury and lipid peroxidation product (MDA) and brought back the liver antioxidants and the over accumulation lipids in serum towards normal. Histopathology of the liver confirmed the changes induced by high fat diet and the heptoprotective effect of Crocin. Crocin exerts protective effects against hepatic steatosis in rats fed with high fat diet possibly through its antioxidant actions.
  Daryoush Mohajeri and Ramin Kaffash Elahi
  Tuberculosis continues to be a common health problem worldwide. Isoniazid, an antibiotic used routinely for tuberculosis chemotherapy is documented to be a potent hepatotoxicant. The aim of the present study was to assess the hepatoprotective activity of green tea (Camellia sinensis) extract (GTE) against isoniazid induced hepatotoxicity in the rats. Male Wistar rats were randomly allocated into 4 groups of 10 animals each including: normal healthy control rats, healthy rats receiving GTE, toxicant control and toxicant drug plus GTE treatment group. In groups 2 and 4 GTE (1.5%, w/v) was given as only source of drinking for 8 weeks. In the midst stage of experiment (4th and 5th weeks), Isonizid (50 mg kg-1 b.w./day, i.p.) was administrated for groups 3 and 4 for a period of 2 weeks. At the end of experiment, serum biomarkers of liver tissue injury and product of lipid peroxidation (MDA), activities of Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPX) and Glutathione Reductase (GR) were assayed in liver homogenates. Finally, the biochemical findings were matched with histopathological verifications. Significant differences among the groups were determined by one-way analysis of variance followed by Tukey post-test. In group 4, GTE significantly (p<0.05) decreased the elevated levels of serum biomarkers of hepatic injury and total bilirubin and significantly (p<0.05) increased the reduced levels of serum albumin and total proteins (respectively p = 0.001, p = 0.032). In this group, GTE significantly (p<0.05) decreased the lipid peroxidation and elevated the decreased values of hepatic antioxidants. Histopathologically, the changes were in the same direction with biochemical findings. This study showed that the hepatoprotective effect of GTE in isoniazid-induced oxidative damage may be related to its antioxidant and free radical scavenging activity.
  Daryoush Mohajeri , Ghafour Mousavi and Yousef Doustar
  Adequate characterization of hypoglycemic effect of ethanolic saffron extract has not been yet done, though the activity has been reported. The scientific evaluation of its hypoglycemic activity was, therefore, explored and compared with the effect of a standard hypoglycemic drug, tolbutamide. In this study, we also report on alteration in patterns of pancreatic islet cells using histopathology and immunohistochemistry of alloxanized diabetic rats treated with ethanolic saffron extract. The ethanolic extract of Crocus sativus L. stigma was administered orally and intraperitoneally at different doses (20, 40 and 80 mg kg-1) to normal rats for finding the more effective hypoglycemic dose and administration route. Acute hypoglycemic effects produced by more effective dose of ethanolic saffron extract on the Fasting Blood Glucose (FBG) levels and effects of the same dose of ethanolic saffron extract on the FBG and plasma insulin levels in alloxanized Mild Diabetic (MD) and Severely Diabetic (SD) rats were assayed. Histopathological and immunohistochemical studies were also carried out on pancreatic islet cells of control and diabetic rats. The dose of 40 mg kg-1 was found to be more effective dose in intraperitoneally (i.p.) route for decreasing Blood Glucose Level (BGL). The extract administered by i.p. route at more effective dose showed an acute hypoglycemic effect in MD and SD rats. Treatment of MD and SD rats for 14 days with the more effective dose significantly reduced the FBG levels in these animals (41.4% MD, 30.7% SD). Serum insulin level showed significant increase in diabetic rats (33.3% MD, 27.3% SD) after 14 days. The histopathological studies of pancreas in ethanolic extract treated diabetic groups showed a reversed damage caused by alloxan to the pancreatic islets as almost normal appearance. In addition, diabetic (MD and SD) rats showed obvious decreases in insulin immunoreactivity and the number of β-cells in pancreas, but the pancreas of extract-treated diabetic rats was improved and the number of immunoreactive β-cells was significantly increased. The control group given saffron extract was not different from the other intact control group considering the insulin immunoreactivity in β-cells. The findings of present study indicate the hypoglycemic and potential antihyperglycemic nature of the extract, helping in regeneration of damaged pancreas in experimental diabetes. Thus, after randomized clinical trials, saffron extract may be implicated as a preventive or therapeutic agent against diabetes mellitus.
  Ali Rezaie , Babak Lotfollahi , Daryoush Mohajeri , Ghafour Mousavi , Mehrdad Nazeri , Solmaz Zakhireh and Babak Mohammadi
  Acceleration in healing of surgical skin wounds, in order to reduction of post-operation consequences is most important. One of the most popular compounds used ever for skin care is lavender oil or Lavandula stoechas essential oil. The aim of this study was evaluate the healing effects of Lavandula stoechas extract in comparison with zinc oxide ointment on full thickness experimental open surgical skin wound healing in the rats. For this purpose, 75 female Wistar rats were randomly assigned in 5 groups of 15 animals each including: Control, treated by Eucerin as the placebo, treated by zinc oxide topical ointment and two remaining as test groups which received 10 and 20% Lavandula stoechas extracts. Under the general anesthesia and analgesia circumstances, a full thickness incisional open wound with 23 mm diameter was made on the skin of the back part of the rats. The healing process was studied macroscopically and microscopically at 0 (the day of surgery) 3, 7, 14, 21 and 28th days of experiment in all of the rats. The greatest degree of wound contraction was considered in Lavandula stoechas extract (10%) treated rats while this change was slighter in control group. In microscopic examination, the overall healing process of Lavandula stoechas extract (10%) treated rats was significantly better than the other experimental groups (p<0.05). The results obtained showed that Lavandula stoechas extract (10%) has more effective healing properties on full thickness open skin wounds compared to zinc oxide topical ointment.
  Daryoush Mohajeri , Ghafour Mousavi and Mehrdad Nazeri
  The thyroid gland in most animal species has two lobes, one on each lateral surface of the trachea. In pigs, the main lobe of the thyroid gland is on the midline in the ventral cervical region with dorsolateral projections from each side. The thyroid structural changes in gouts were investigated. About 386 pairs of thyroid glands collected from gout at the local municipal abattoir in Tabriz city of East Azerbaijan province. A total of 386 thyroid specimens examined, 8 cases were follicular atrophy, 4 cases were thyroid fibrosis, 71 cases were diffuse hyperplasia of thyroid follicular cells, 5 cases were colloid goiter, 10 cases were parenchymal cysts, 3 cases were nodular hyperplasia, 1 case was C-cell adenoma and 1 case was C-cell carcinoma.
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