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Articles by D.B. James
Total Records ( 2 ) for D.B. James
  D.B. James , D.A. Ameh and A.S. Agbaji
  Neem seed cake resulting after solvent extraction with water and different concentrations (35, 55, 75% and absolute) of methanol, ethanol and propanol were analyzed for crude protein, in vitro protein digestibility and amino acid composition Processed neem seed cake obtained from 55% methanol, 35% propanol and 100% propanol was significantly (p<0.05) lower in crude protein content compared to that obtained through water extraction while 75% ethanolic treated neem seed was significant (p<0.05) higher. There was a significant (p<0.05) increase in vitro protein digestibility of neem seed cake processed with 75% methanol over that with water. Neem seed cake obtained by extraction with 75% methanol, 75% ethanol and 55% propanol compared favorably with the amino acid profile to soya bean protein. The total essential amino acid content of neem seed cake obtained from 75% methanol was higher than that obtained for soya bean protein. The nutritional potential of neem seed cake, on the basis of these results, is discussed.
  D.B. James , E.A. Abu , A.U. Wurochekke and G.N. Orji
  The root, stem bark and leaf of Ximenia americana from the Olacaceae family which is used as herbal remedies for the cure of many ailments by natives in the Northern part of Nigeria was studied. The aqueous and methanolic extracts of the afore mentioned parts of the plant were subjected to phytochemical screening and antibacterial evaluation. From the tests carried out, it was observed that the extracts contained carbohydrates in the form of sugars and soluble starch, cardiac glycosides, saponins, tannins and flavonoids while alkaloids were absent in all the extracts. Anthraquinones were present in all the extracts except the leaf extracts. The extracts were tested against five bacteria in which they inhibited the growth of Staphylococcus aureus and Klebsiella pneumoniae while Shigella flexneri was inhibited by only the methanolic leaf, aqueous bark and aqueous leaf extracts. Salmonella typhi and Escherichia coli were not affected by these extracts. The Minimum Inhibitory Concentration (MIC) was only evident for the methanolic extracts at 1.25x104 μg mL-1 (l:4) against Staphylococcus aureus while the Minimum Bactericidal Concentration (MBC) of these extracts on Staphylococcus aureus was obtained at 2.50x104 μg mL-1 (1:2).
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