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Articles by Constantin BOTEZ
Total Records ( 4 ) for Constantin BOTEZ
  Erika BALAZS , Constantin BOTEZ , Abdelmoumen TAOUTAOU , Dana CURTICIU , Daniela BRICIU , Laura Cristina COTA and Alexandru BRICIU
  Cultivated potato is susceptible to many pests and pathogens, none of which is more of a threat to potato agriculture than the late blight disease, caused by the oomycete Phytophthora infestans (Mont.) de Bary. Resistant R genes from the wild species of Solanum demissum have been used by breeders to generate late-blight-resistant cultivars. In our research we tried to clone polymorphic bands that could be considered markers for resistant genes. We used 8 resistant potato varieties and one sensible form. After amplification of potato DNA with decamer primers, several polymorphic RAPD sequences were recuperated from the agarose gel, and cloned into E. coli bacteria using two cloning kits. The majority of cloned sequences had approximately the same size with the original recuperated sequence.
  Erika BALAZS , Constantin BOTEZ and Abdelmoumen TAOUTAOU
  Phytophtora infestans, the causal organism of late blight, is the most important fungal pathogen of potato. In the strategy of potato late blight control utilization of resistant variety is the most important. For creating new resistant varieties Marker Assisted Selection increase significant the efficiency of this process. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. We tested 8 primers for RB gene homologues from Solanum bulbocastanum on different potato species, including S. bulbocastanum, S. demissum, and S. tuberosum. Some primers amplified only S. bulbocastanum, and others amplified olso S. demissum and S. tuberosum.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL. , Erika BALAZS , Constantin BOTEZ , Adina CHIS and H. MATEI
  We used the 1D SDS-PAGE to study the compatible interaction P. infestans- Solanum tuberosum. A virulent isolate has been used to colonize the cultivars Bintje and Desiree and another genotype with the R2 resistance gene. Two proteins were induced in the case of bintje: 14kD parotein and metallocarboxypeptidase inhibitor. In the case of R2, three proteins were induced with two not identified. The two identified were: β-glucosidase and transcription activator PTI6.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL , Erika BALAZS and Constantin BOTEZ
  Not available
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