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Articles by Chenggang Duan
Total Records ( 2 ) for Chenggang Duan
  Haiqing Yu , Chun Zhang , Zhiqiang Mei , Li Wang , Juan Li , Chenggang Duan , Xiaoyan Liu , Shu Gong , Lin Gan , Zhonghua Tao , Fuli Yao , Ye Yang , Chunyan Duan , Youping Liu , Chunyan Zhang , Chuanning Chen , Xiaoli Zheng , Hongxian Zhao , Jiyan Cheng , Xiaojun Tang , Yong Zhang , Yingxi Zhang and Junjiang Fu
  As a traditional medicinal herb in China, Penthorum chinense Push from Gulin county is regarded as genuine regional drug with better clinical effects. To discriminate the geographical origin of six P. chinense samples cultivated in three provinces, Randomly Amplified Polymorphic DNA (RAPD) analysis was carried out with an improved method to increase the resolution and production using 10 mer-random primers. Similarity index was ranged from 0.61 to 0.97, which demonstrated that samples from different localities displayed similar band patterns. However, based on the analysis of selected 13 primers, primers SBS-I9, SBS-I20 and SBS-Q9 produced distinguishable bands among Sichuan, Yunnan and Hubei P. chinense. T-test of mean S.I. values of six accessions demonstrated the significant differences between Luzhou or Sichuan samples and others. Cluster analysis indicated that cultivars with close geographic distributions were clustered together consequently. This suggested that there are RAPD site variations and local specialized genotypes among the six samples. The results indicated that RAPD analysis is effective in distinguishing the geographical origin of genuine regional drug P. chinense grown in Luhzou, Sichuan. The approach is a valuable tool to authenticate other morphologically similar herbal medicinal materials.
  Chenggang Duan , Zhiqiang Mei , Shu Gong and Haiqing Yu
  Penthorum sedoides L. and Penthorum chinense Push is closely related aneuploids in genus Penthorum L. based on the previous data. However, P. chinense are treated as variety or subspecies of P. sedoides successively by botanists and little was known about their relationships on molecular level. An improved randomly amplified polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were performed to obtain their genetic characterizations and species-specific DNA fragments. Obviously, five 10-mer random primers SBS-A3, SBS-I3, SBS-I18, SBS-M6 and SBS-Q9 demonstrated different fingerprints. Six candidate specific bands (I3-PS, I3-PC, I18-PC, M6-PS, Q9-PS and Q9-PC) displayed in two taxa were successfully cloned and sequenced. Based on these sequences, six pairs of SCAR primers from P. sedoides (I3PSF/I3PSR, M6PSF/M6PSR and Q9PSF/Q9PSR) and P. chinense (I3PCF/I3PCR, I18PCF/I18PCR and Q9PCF/Q9PCR) were designed, respectively. However, four of six primer pairs yielded amplicons common in the two taxa. The DNA amplification using Q9PSF/Q9PSR primers generated a single 555 bp band only in P. sedoides and Q9PCF/Q9PCR primers produced a 760 bp fragment unique to P. chinense. The results suggested that P. sedoides and P. chinense are still closely related, although there are a lot of variations in RAPD genetic sites. The developed RAPD and SCAR techniques are effective and useful in revealing genetic characterizations of P. sedoides and P. chinense. Also, the SCAR analysis based on the improved RAPD method is powerful in authentication of species with close relationships.
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