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Articles by Chen Hong
Total Records ( 4 ) for Chen Hong
  Liu Wu Jun , Fang Yi , Shao Yong-gang , Fang Guang Xin , Tian Ke Chuan , Huang Xi Xia , Chen Hong and Zhang Fu Quan
  In this study, a novel deletion mutation in the whole CDS region at cashmere goat KAP16.5 gene locus is reported, which result showed the deletion of KAP16.5 gene in 816 cashmere goat samples was firstly detected in China. At the same time, parts of these samples sequenced. However, there are not many studies of goat KAP16.5 gene and its polymorphisms in literary sources. The polymorphism was studied in three Xinjiang local goat breeds of China. The results showed that there was 15 bp-deletion mutation in this gene. The frequencies of the KAP16.5-Y allele in Xinjiang goat (n = 220), Nanjiang cashmere goat (n = 310) and BoGeDa cashmere goat breeds (n = 286) were 0.9591, 0.8694 and 0.8846, respectively. The χ2-test showed that the genotype distributions in these three cashmere goat breeds were not in agreement with Hardy-Weinberg equilibrium. According to the classification of PIC, Nanjiang cashmere goat was more polymorphic at this locus. Moreover, the 15 bp-deletion mutation was described at KAP16.5 locus for the first time and not have described at KAP16.5 locus. Then, the deletion polymorphisms within the KAP16.5 have been associated with economic production traits data in Nanjiang cashmere goat breed shown no significant (p>0.05).
  Chen Hong , Jiang Tie and Zhu Gui-Bin
  In order to reduce the time of the process of feature description in SURF algorithm and improve the speed of SURF algorithm, an improved SURF algorithm based on image entropy is proposed. It uses image blocks in the overlap region of original images. Then the image entropy of each block is calculated to select reliable area. The reliable area is only used to detect feature points. The two methods (Uniform sampling and the distance constraint between feature points) are used to control the feature points number. The speed of SURF algorithm is increased by about 65% and the performance is also improved.
  Liu Wu-Jun , Fang Guang-Xin , Fang Yi , Tian Ke-Chuan , Huang Xi-Xia , Yao Xin-Kui , Wang Mou , Yu Hui , Huang Yong-Zhen , Xin Jing-Jing , Xin Ya-Ping , Yu Shi-Gang and Chen Hong
  Searching for effects of candidate gene polymorphisms on cashmere production traits is an important goal for goat industry. Genetic variations in IGF-1 may alter protein function. This study investigated the association between polymorphisms in insulin-like growth factor-1 (IGF-1) and cashmere traits data with two Xinjiang local goat breeds. The results showed IGF-1-P1 locus has polymorphisms. The polymorphism locus of IGF-1-P1 by PCR-RFLP and DNA sequencing methods in 530 individuals from two Xinjiang local goat breeds in China. The frequencies of genotype AA in two goat breeds (Xinjiang goat, Nanjiang cashmere goat) were 0.487 and 0.277. Genotype BB was 0.274 and 0.486. Genotype AB was 0.239 and 0.236. The polymorphisms of the IGF-1 gene were associated with cashmere yield, fiber diameter, body weight in cashmere goat. However, concern on cashmere production traits among three genotypes were shown not significantly (p>0.05).
  Fang Xing-Tang , Xu Hai-Xia , Chen Hong , Zhang Chun-Lei , Hu Xiu-Cai , Gao Xue-Yuan , Gu Chuan-Wen , Yue Wang-Ping and Lan Xian-Yong
  Bone Morphogenetic Protein 4 (BMP4) plays a crucial role in development and productivity of mammalian. The aim of the present study was to identify and characterize polymorphisms within the coding region, the intron region and the 3'flanking region of the goat BMP4 gene in three different breeds. Three DNA fragments were amplified by Polymerase Chain Reaction (PCR) and then used for polymorphism identification by Single Stranded Conformation Polymorphism (SSCP). The fragments showing different SSCP patterns were sequenced. As a result, two new SNPs (EU104684:g.1986A>G, 2203G>A) were identified in the intron region and a short sequence with more than ten continuous and repeated CA dinucleotide were found in the 3'flanking region near the termination site of coding region and the length of repeated sequence dinucleotides CA was different in samples. Clonal sequencing analysis indicated that it was a microsatellite of dinucleotide-repeated sequence. The lengths of repeated sequence dinucleotide CA were 24, 17, 14, respectively. The PIC (Polymorphism Information Content) values in three populations were 0.3656, 0.5792, 0.3103 for Xuhuai White goat, Boer goat and Haimen goat, respectively, which indicated this goat microsatellite locus had rich polymorphism.
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