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Articles by Chao-Yun Li
Total Records ( 2 ) for Chao-Yun Li
  Chao-Yun Li , Xiang-Hui Zhao , Yang-Chun Cao , Yao-Geng Lei , Chan-Juan Liu , Hua-Xin Wang and Jun-Hu Yao
  This study was conducted to investigate the effects of chitosan on ruminal fermentation, methane production and microbial populations in diets with different Forage to Concentrate (F:C) ratios in vitro. A two factorial in vitro experiment was designed with one factor as three F:C ratios (80:20, 50:50 and 20:80) diets and the other as four levels of chitosan in rumen-buffer fluid (0, 333, 667 and 1000 mg L-1). After 24 h incubation, the results showed that chitosan significantly decreased In Vitro Dry Matter Disappearance (IVDMD) (p<0.001), total gas production (p<0.001), methane production (p<0.001) and Acetate:Propionate (A:P) (p = 0.001) and significantly increased the rumen pH (p<0.001). Chitosan significantly increased the molar proportion of propionate of rumen-buffer fluid in F:C ratios of 80:20 (p = 0.009) and 50:50 (p = 0.007). In F:C ratios of 20:80, chitosan significantly increased the ammonia-N concentration (p = 0.013) and the molar proportion of butyrate (p<0.001) and significantly decreased the molar proportion of acetate (p = 0.001). In conclusion, chitosan affected the ruminal fermentation and reduced methane production of rumen-buffer fluid in vitro.
  Chao-Yun Li , Yang-Chun Cao , Shi-Zhao Li , Ming Xu , Chan-Juan Liu , Zhi-Peng Yu , Xiang-Hui Zhao and Jun-Hu Yao
  This research was conducted to investigate the effects of exogenous fibrolytic enzyme on in vitro ruminal fermentation and microbial populations with substrates in different Forage to Concentrate ratios (F:C). Four levels (0, 40, 80 and 120 U g-1 dry matter substrate) of fibrolytic enzyme were supplemented to buffered rumen fluid and incubated with three different F:C ratios (80:20, 50:50 and 20:80) substrates at 39°C. After 24 h incubation, the results showed that in three different F:C ratios, fibrolytic enzyme significantly increased (p<0.001) total gas production, in vitro dry matter disappearance, concentration of total volatile fatty acid and molar proportion of propionate and decreased (p<0.001) pH but it did not significantly (p>0.05) affect molar proportion of butyrate. And in the high (F:C 80:20) and medium (F:C 50:50) forage substrates, fibrolytic enzyme increased (p<0.05) the molar proportion of propionate and decreased (p<0.05) the ratio of acetate to propionate but the effects did not observed in high concentrate (F:C 20:80) substrates. It was also found that fibrolytic enzyme increased (p<0.05) the number of Fibrobacter succinogenes and methanogens in the F:C 80:20 substrate but there was no significant (p>0.05) effects on microbial populations in the F:C 50:50 and 20:80 substrates. The results indicated that the fibrolytic enzyme improved the degradation of substrates and had different effects on the ruminal fermentation pattern and microbial populations under three F:C ratio substrates. The efficiency of fibrolytic enzyme on high and medium forage substrates was greater than low forage substrates.
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