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Articles by Chandrashekhar G. Joshi
Total Records ( 2 ) for Chandrashekhar G. Joshi
  M. Manjunath Hulikere , Chandrashekhar G. Joshi , T. Nivya , D. Ananda and N.G. Raju
  Background and Objective: Angiogenesis plays a vital role in the formation of new blood vessels from preexisting vasculature during embryonic development processes. Excessive angiogenesis leads to several pathogenic conditions such as cancer, arthritis and psoriasis. Marine natural products are gaining the attention of researchers in recent years as they are the rich source of diverse medicinal compounds. Endophytic fungi from marine seaweeds are less studied and presented as a new subject of investigation of novel therapeutic agents. The aim of the present study is to evaluate antioxidant and antiangiogenic activity of ethyl acetate extract of the endophytic fungi, Penicillium citrinum isolated from Sargassum wightii. Methodology: Endophytic fungi were isolated from seaweed S. wightii. The isolated fungi was identified by 18S rRNA and ITS sequence. The ethyl acetate extract of endophytic fungi was subjected for antioxidant property using 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging assay. The extract was also analyzed for reducing power, total phenolic content and total flavonoid content. Antiangiogenesis potential of the extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by CAM assay. Results: The endophytic fungus isolated from S. wightii was identified as Penicillium citrinum based on the analysis of 18S rRNA and ITS sequence. Ehtyl acetate extract of P. citrium showed a significant antioxidant and angiosuppresive activity. The antioxidant nature of the extracts was found to be concentration dependent. The ESI-LCMS analysis revealed presence of polyphenols at different range. Further studies are in progress to isolate, characterize and elucidate the structure of active compounds. Conclusion: Further studies on the compound present P. citrium extract will help to develop an alternative therapy for cancer menace.
  Chandrashekhar G. Joshi , M. Gopal and N. Suchetha Kumari
  The practice of traditional medicines in treatment of cancer is based on the knowledge and use of plant based medicine since ancient time. The study of folk medicinal practices revealed the use of Tragia involucrata (Euphorbiaceae) along with other medicinal plants to treat certain tumours in some parts of Kerala, India. The aim of the present study was to evaluate the antitumor potential of hexane and ethyl acetate extracts (HE and EAE) and to asses the antioxidant activity of EAE of T. involucrata. The mice were injected with 106 Ehrlich’s Ascites Carcinoma (EAC) cells subcutaneously and the extracts were administered intra-peritoneal (i.p.). In vitro antioxidant property of EAE was tested by 2, 2’-azinobis (3-ethylbenzo-thiozoline-6-sulphonic acid (ABTS)) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. In vivo antioxidant potential of EAE was determined by nitric-oxide by Griess-reagent method, total antioxidant in serum by phosphomolybdenum method and super oxide dismutase by nitroblue tetrazolium (NBT) method. Administration of the extracts to mice challenged with EAC showed significant antitumour activity. The observed antitumor activity of the extracts was dose dependant. There was a significant increase in the percentage life span (ILS %) in extract treated mice. HE was not toxic to mice even at the dose of 2000 mg kg-1 body weight. EAE was toxic to animals when the dosage was increased beyond 150 mg kg-1 . EAE was showing even a significant in vitro as well as in vivo antioxidant activity. In vivo antioxidant activity was showing the reciprocal dose response. From the results it may be concluded that the plant posses antitumor activity as claimed in traditional practices.
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