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Articles by Carmen SOCACIU
Total Records ( 7 ) for Carmen SOCACIU
  Carmen SOCACIU , Monica TRIF , Dumitrita RUGINA , Adela PINTEA and Simion ASTILEAN
  We aimed to demonstrate that the functionalization of nanocolloids of Au in vitro, induce the formation of conjugated forms (with glutathion) which can affect the cellular activity, as tested on Saccharomyces cerevisiae and RPE cells. The cell line relevant for macular degeneration, type RPE (line D407) proved to be more sensitive to nanogold and conjugated forms. By microscopy we demonstrated the cell capacity to form a monolayer, as a prove of their proliferation and viability, as well in the presence of free AuC, glutathione, but also in the presence of conjugated forms AuC-glutathion. AuC-glutathion conjugate, at pH 5.6 (optimum for cell growth) is stable and can have effect on cellular activity, with impact on proliferation and viability. Obviously, the interaction of AuC and AuC-glutation conjugate with cell components may have effects on cell proliferation and rapid metabolization in presence of appropriate enzymes. (e.g. Glutation peroxidase). Alternative and complementary studies are needed to show the localization of AuC and AuC-glutathion conjugate at cellular level, by microscopy and spectroscopy. It is possible that, inside cell, AuC to conjugate other biomolecules, with a higher stability and affinity, comparing to glutathion.
  Carmen SOCACIU , Andreea STANILA , Monica TRIF and Simion ASTILEAN
  Our studies aim the preparation of functionalized Au and Ag colloids invitro, the dynamics of conjugation and stability in functionalized forms (by conjugation with cysteine, glutathione, insulin, albumin) (in this first part of article) as well and to study their action on cells (second part). Such investigations can prove that natural ways of Au and Ag functionalization, by formation of stable conjugates, with S-containing biomolecules can be active at cellular level and can act synergistically as protective, antioxidant agents. We determined dimensions of colloid Au (AuC) vs colloid Ag (AgC) and found out mean values between 0.863μm to 0.86 μm.Using a calibration curve for AuC in the range 350-800 nm, we found a good correlation concentration-absorption units and the best dilutions to be used in conjugation experiments (1:2-1:5 ). The kinetic of AuC conjugation with four different molecules (cystein, BSA, insulin and glutathion), was specifically expressed by shifts of around 3 nm (for cystein, insulin and albumin) suggesting that AuC cannot make directly stable conjugates with these molecules. The same kinetic, tested for AuC-glutathion, showed spectacular change of color from red to blue and shifts of the absorbtion of conjugated forms, up to 16 nm (from 600 to 616 nm). Contrasting to Au, AgC in the presence of glutathion did not show modifications of absorptions, nor color changes. Therefore, we consider that Ag cannot conjugate glutathion and it is not useful to test it on cell cultures in vitro. Using different pHs (from 3 to 8) we observed an increase of conjugation capacity of AuC with glutathione, up to values 7-7.5. At extreme values (pH 3 and 8) the conjugation is not possible.
  Adriana PAUCEAN , Dan VODNAR , Elena MUDURA , Mirela JIMBOREAN , Carmen SOCACIU and Dorina BRATFALEAN
  Dairy products may go beyond the borders of nutritional value and become functional products if the fermentation process is performed in the best available manner. Our study monitories the process parameters and their variation during the fermentation time of milk incubated with a complex mixture of microorganisms formed by: a lactic culture, kefir-yeast culture and brewer’s yeasts sampled from the secondary fermentation of beer. The study was realized using a Tryton- Pierre Guerin Technologies biofermentor. The results were correlated with the development dynamics of lactic bacteria and yeasts population during 24 hr of fermentation. The pH level variation is typical for kefir-like products. The oxygen content during fermentation is a competitive factor for the two species of microorganisms featuring in our research: Lactococcus lactis and Saccharomyces cerevisiae. In this situation the semi-anaerobic environment insures the fastest rate of acidification for milk and the Saccharomyces cerevisiae yeasts did not display a fermentation metabolism but maintain their viability.
  Adriana PAUCEAN , Dan VODNAR , Elena MUDURA , Mirela JIMBOREAN and Carmen SOCACIU
  We used sensory analysis to select the optimum sample from 11 different samples obtained by inoculating 1.8% skimmed milk with complex mixtures of microorganisms containing lactobacilli, kefir yeast and brewer’s yeasts in different quantities. We used the following tests: the triangle test, the hedonic test, the score test, and the flavour profile test, for a panel-group made up of ten people without previous training as tasters, aged 13-55. Tasters were selected following a discussion which assessed their potential and availability. We took into account the provisions of SR 6345, regulating sensory analysis of milk and milk products. The sensory analysis was performed in two stages: during the first stage we aimed to optimize the quality and quantity of the inoculators, and during the second stage we described the end product. The analysis was performed during the shelf-life of product (1-21 days) stored at 4-6°C. The triangle test showed that there are significant differences in taste between the brewer’s yeast product and the control sample (yeast free). The hedonic test showed that the acceptability of the product is at its peak during the first seven days of the shelf life, at a temperature of 4-6°C, and then decreases slowly until the expiry date. We used statistical analysis to pick the sample with the best acceptability throughout the shelf life. The score test showed that the product obtained after the optimization process has specific positive, well-developed organoleptic features, and no noticeable flaws. The flavour profile test identified the main taste and odour markers of the product and their degrees of intensity; the taste and odour of yeast was considered moderate until the expiry date. Sensory analysis confirmed the choice of this insemination method as the best from the point of view of sensory properties.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL. , Erika BALAZS , Constantin BOTEZ , Adina CHIS and H. MATEI
  We used the 1D SDS-PAGE to study the compatible interaction P. infestans- Solanum tuberosum. A virulent isolate has been used to colonize the cultivars Bintje and Desiree and another genotype with the R2 resistance gene. Two proteins were induced in the case of bintje: 14kD parotein and metallocarboxypeptidase inhibitor. In the case of R2, three proteins were induced with two not identified. The two identified were: β-glucosidase and transcription activator PTI6.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL , Erika BALAZS and Constantin BOTEZ
  Not available
  Dumitrita RUGINA , Simona VICAS , Mariana PETRAN , Adela PINTEA , Andrea BUNEA and Carmen SOCACIU
  In Europe, mistletoe extract (Viscum album) is widely used as complementary cancer therapies. The main active component of mistletoe extract is glycoprotein, lectin, that exhibit cytotoxic effects and immunomodulatory activities. The aim of this study was to show if the mistletoe extract, rich in phenolic acids presents anti-carcinogenic potential on A2780 tumor ovarian cells. In the first part of this study, using high performance liquid chromatography (HPLC) analysis we characterized phenolic acid content of mistletoe growing on Malus domestica. And, in the second part, we investigated whether by apoptosis on ovarian tumor cell line A2780 if anti-cancer effect of this extract mistletoe is induced. Cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. The A2780 cell viability was inhibited to 87% by 50 μg/ml mistletoe extract treatment, and to 79% at 110 μg/ml extract concentration. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining was performed. Cells treated with mistletoe (110 μg/ml) for 24 h displayed typical morphological features of apoptotic cells, with condensed and fragmented nuclei. Fluorimetric method was used to evaluate total reactive oxygen species content from A2780 ovarian tumor cells after mistletoe treatment. Mistletoe concentration 70μg/ml induces a significant toxic ROS-generating effect. These data indicates that the phenolic acids from mistletoe induce cell death by apoptosis on human tumor ovarian cell line, A2780.
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