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Articles by C. Rivas-Morales
Total Records ( 3 ) for C. Rivas-Morales
  P. Garcia-Palencia , M.A. de la Garza-Ramos , S.A. Galindo-Rodriguez , A. Oranday-Cardenas , C. Leos-Rivas , M.J. Verde-Star and C. Rivas-Morales
  Background and Objective: Periodontal disease and dental caries are frequent oral illnesses. Both have an important impact on quality of life are of polymicrobial origin and progress slowly. Plants are a valuable resource in health systems in developing countries and a large part of traditional treatments involve the use of plant extracts or their active substances. The objective of this study is to determine the antimicrobial activity of the methanolic extract of Psacalium decompositum using the plate diffusion method in vitro and to determine the minimum inhibitory concentration of Porphyromonas gingivalis, Prevotella intermedia and Streptococcus mutans. Materials and Methods: The plant was collected and identified, Psacalium decompositum and a methanol extract was prepared by mashing. Phytochemical screening was also performed. The methanol extract was evaluted by the plate diffusion method using different microdilutions in broth against the ATCC strains Porphyromonas gingivalis, Prevotella intermedia and Streptococcus mutans. About 0.2% chlorhexidine was used as a positive control and 5% ethanol as a negative control. The nonparametric statistic, the Kruskal-Wallis test was used to compare the three treatments and the Mann-Whitney test for each pair of treatments to identify significant differences between them. Results: The minimum inhibitory concentration of the methanolic extract of P. decompositum was 500 μg mL–1 for P. gingivalis and P. intermedia and 700 μg mL–1 for S. mutans (p<0.002). The minimum inhibitory concentration for chlorhexidine (positive control) was 900 μg mL–1 for the three bacteria studied. Conclusion: The methanolic extract was active against cariogenic and periodontopathogenic bacteria and represents a natural alternative for the control of these microorganisms that produce oral diseases. The methanolic extract of P. decompositum has antimicrobial activity making it a natural alternative for the treatment of caries and periodontal disease.
  C. Rivas-Morales , V.M. Rivas-Galindo , J. Rodriguez-Rodriguez , S.A. Galindo-Rodriguez , C. Leos-Rivas and D.G. Garcia-Hernandez
  Background and Objective: Plants are used in Mexico as traditional medicine for the treatment of diverse illnesses such as stomach pain, fever, diarrhea, insomnia, flu and other respiratory diseases. Twenty were selected to determine their bactericidal activity. The aim of this study was the isolation of molecules from plants used in Mexican traditional medicine. Materials and Methods: Using chromatographic procedures, the responsible bactericidal molecules from rosemary was extracted and then identified by spectroscopic analysis IR, 1H NMR, 13C NMR, DEPT, HSQC and GC-MS. Measures of central tendency were determined by statistical analysis. Results: Ten of these plants showed bactericidal activity against multidrug-resistant bacteria. This biological activity was reported for Carya illinoensis against Pseudomonas aeruginosa, also for Equisetum robustum, Stevia rebaudiana and Castela texana against Methicillin resistant Staphylococcus aureus (MRSA). The methanolic extract of Rosmarinus officinalis (rosemary) showed important bactericidal activity against MRSA (ATCC BAA-44) and clinically isolated MRSA. Conclusion: Rosemary’s bactericidal molecules were isolated and then identified as a mixture of betulinic, oleanolic and ursolic acid (MIC = 725 μg mL–1).
  E. Viveros-Valdez , C. Rivas-Morales , A. Oranday-Cardenas , M.J. Verde-Star and P. Carranza-Rosales
  Hedeoma drummondii is a medicinal plant with diverse properties; however, validations of its medicinal uses are scarce. To evaluate its antimicrobial properties H. drummondii, was tested against opportunistic pathogens of medical importance. Antimicrobial tests were performed by the microdilution method in order to determine the Minimal Inhibitory Concentration (MIC) for each extract. Extracts of this plant showed relevant antimicrobial activity, results revealed that the hexanic extract has stronger activity and broader spectrum compared to acetone and methanol extracts. The activity of hexanic extract may be attributed mainly to the presence of the monoterpenes pulegone and menthol. In conclusion, the hexanic extract possess relevant antibacterial properties which suggests that H. drummondii have bioactive principles; these new data provide scientific support for the use of this plant in traditional medicine, particularly for gastrointestinal diseases.
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