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Articles by B.E. Boboye
Total Records ( 3 ) for B.E. Boboye
  F.O. Omoya , B.E. Boboye and F.A. Akinyosoye
  The type of bacteria present on two insects (cockroach and mosquito) were investigated. These microorganisms were screened for microbial control of mosquito employing their degradative ability at various microbial cell loads. The degradation of the mosquito was observed spectrophotometrically for an incubation period of 5 to 7 days. Six bacterial species (Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Serratia marcescens, Staphylococcus aureus and Aerobacter aerogenes) were isolated from cockroach. Mosquito borne E. coli, P. aeruginosa, B. cereus and Staph. aureus. All the bacteria digested the mosquito with activity ranging from 0.02 to 1.27. Microorganisms associated with cockroach showed higher degradation activity (0.02-1.27) during the incubation than those obtained from mosquito (0.02-1.00).
  T.A. Akintunde , O.P. Abioye , S.B. Oyeleke , B.E. Boboye and U.J. J. Ijah
  Pseudomonas aeruginosa was isolated from 20 years abandoned mine site of Itagunmodi Atakunmosa West, Ilesha, Nigeria. Atomic absorption Spectrophotometry (AAS) revealed Fe, Mn, Cr, Zn, Pb and Cr, while, Fe has the highest concentration range of 29-289 ppm in the analysed soil samples. Soil samples were enriched in R2b agar, serially diluted and pour plated. Four bacteria strains were isolated and identified using standard biochemical test. After routine biosurfactant screening by oil spreading and emulsification test, biosurfactant producing bacteria was confirmed as Pseudomonas aeruginosa. The partially purified biosurfactants were characterized with TLC and GC-MS analysis. The analyses indicated glycolipid biosurfactant specifically designated as Rhamnolipid-sa1 containing isopalmitic acid, hexadecanoic acid, methyl ester and hydroxylated fatty acid linked to decanoic acids. Iron removal potential of the extracted biosurfactant was studied and the result revealed that Rhamnolipid-sa1 effectively reduced iron (60.34%) and could be useful as alternative remediation tool for treatment of iron contaminated soil.
  B.E. Boboye and A.J. Alli
  Effects of garlic extract at 67, 134 and 201 mg mL-1 on Pseudomonas aeruginosa and Staphylococcus aureus were studied. In the absence of the extract, the cells grew to high densities within 11/2 h at 37 °C. Garlic extract-treated cells reduced in number and died. Percentage living cells at 201 mg mL-1 was 0% for both bacteria. Sucrose and MgSO4 stabilized and protected the cells. At 67, 134 and 201 mg mL-1 of the extract in the presence of this sugar and the compound, 47, 4 and 0% of Ps. aeruginosa cells were viable. Microscopic examination of carbol fuschin and Giemsa stained cells showed that the garlic treated cells were bigger in size than those of untreated ones; and intact and definite nuclei were lacking. The cell wall was the target of attack and the extract was bacteriolytic in action.
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