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Articles by B.A. Adeniyi
Total Records ( 6 ) for B.A. Adeniyi
  B.A. Adeniyi , F.A. Ayeni and S.T. Ogunbanwo
  Five Lactic Acid Bacteria (LAB) were isolated from different indigenous fermented dairy foods and identified as Lactobacillus fermentum, L. brevis, L. plantarum, Lactococcus lactis and Streptococcus durans. The cell free supernatant of the selected LAB were able to inhibit the growth of all organisms implicated in urinary tract infection (UTI) used in this study. The largest zone of inhibition was produced by Lac. lactis K3 against Staphylococcus saprophyticus UCH 2051. The sensitivity of UTI pathogens to different antibiotics was investigated. The entire Gram-negative pathogens from UTI showed 100% resistance to Colistin, Augmentin, Nalidix acid, Nitrofuranton and Cotrimoxazole while most Gram positive pathogens from UTI were sensitive to different antibiotics used against them. The tested LAB produced various antimicrobial compounds such as organic acid, hydrogen peroxide and diacetyl. The highest yield of lactic acid (1.87 g L-1) was produced by Streptococcus durans K4 while the highest yield of diacetyl (2.324 g L-1) was produced by L. brevis M5 and the highest yield of hydrogen peroxide (0.00544 g L-1) was produced by L. plantarum N2.
  B.A. Adeniyi , R.O. Odufowoke and S.B. Olaleye
  The antibacterial and gastroprotective properties of crude extracts of Eucalyptus torelliana were investigated. Antibacterial activity was investigated by screening the crude extracts for activity against clinically isolated strains of wound bacteria viz., Staphylococcus aureus UCH 2010, Pseudomonas aeruginosa UCH 2125, Escherichia coli UCH 2007, Klebsiella species UCH 2694 and Proteus mirabilis CHO 2014. The dichloromethane crude extracts demonstrated highest antibacterial activity against all tested microorganisms at 10 mg mL-1 concentration. The gastroprotective effect of the crude extract of the leaf was investigated in albino rats. This was evaluated against gastric mucosal damage induced by ethanol/HCl mixture. Ethanol/HCl mixture (1.5 mL of 0.15 N HCl in 70% ethanol) caused severe gastric mucosal damage with ulcer index of 2.7±0.33. Pre-treatment of animals with crude extract of Eucalyptus torelliana leaf 200 and 1000 mg kg-1 orally for 1h significantly reduced the Pre-treatment of animals with 50 mg kg -1. Ranitidine for 1 h reduced the reduced the formation of ulcer by the ethanol HCl mixture with preventive ratios 56 and 92.5%, respectively. Ranitidine (50 mg kg-1) afforded 92.5% protection. The results therefore suggest that crude extracts of Eucalyptus torelliana possess both antibacterial and gastroprotective properties.
  O.O. Aiyelaagbe , B.A. Adeniyi , O.F. Fatunsin and B.D. Arimah
  Jatropha curcas is an ornamental plant which is also employed to cure various infections in traditional medicine. The hexane, ethyl acetate and methanol extracts of this plant were analysed phytochemically and screened against different microorganisms responsible for various infections especially sexually transmitted diseases. Phytochemical analysis of the extracts revealed the presence of many secondary metabolites including steroids, alkaloids and saponins. The extracts and purified fractions displayed potent antimicrobial activity against the target organisms giving MIC as low as 0.75 μg mL-1. The results confirmed the potency of this plant in treating infections including sexually transmitted infections.
  T.E. Lawal , E.A. Iyayi , B.A. Adeniyi and O.A. Adaramoye
  Enzymes were extracted from four fungi: Aspergillus niger, Trichoderma viride, Rhizopus stolonifer and Mucor mucedo. The purified enzyme extracts were used to degrade Groundnut Pod (GNP) in solid state. Undegraded GNP-, degraded GNP- and Roxazyme G2G-based diets were fed to starter and finisher broilers at the rates 70 and 100 g kg-1 of diet, respectively. There was a production of a broad spectrum of enzymes from the 4 fungi. Treatment of the GNP with the fungal enzyme extracts caused a more significant (p<0.05) reduction in the crude fibre and complex carbohydrate fractions and an increase in the crude protein, metabolizable energy and phosphorus in the GNP compared to the undegraded and Roxazyme treated GNP. The amounts of glucose, fructose, galactose and sucrose in the GNP were significantly (p<0.05) increased on biodegradation with the fungal enzyme extracts. Enzyme extracts from M. mucedo and R. stolonifer were more superior in this regard compared to extracts from the other fungi. Diets containing the degraded GNP resulted in significantly (p<0.05) reduced viscosity, better apparent nutrient digestibility and performance in broilers compared to the other diets. Results suggest the possibility of production of a multienzyme complex from some common tropical fungi. These enzyme complexes are more effective in biodegrading complex carbohydrates of by-products like GNP than Roxazyme which is specific for cereal based diets.
  T.E. Lawal , E.A. Iyayi , B.A. Adeniyi and O.A. Adaramoye
  Palm Kernel Cake (PKC) was used as a substrate to elicit the production of polysaccharidases from Aspergillus niger, Trichoderma viride, Rhizopus stolonifer and Mucor mucedo. The extracted enzymes produced were purified and used to ferment PKC in solid state at the rate of 250 ml/kg of the material for 7 days. Unbiodegraded and enzyme degraded PKC were used to formulate broiler starter and finisher diets at the rates of 70 g kg-1 and 100 g kg-1, respectively. A 6th diet was formulated in which Roxazyme G2G, a commercial enzyme was used to supplement the unbiodegraded PKC at the recommended inclusion level of 0.15 g kg-1. A total of 360 1-d-old broiler chicks were randomly allocated to the 6 treatments of 6 replicates each with each replicates having 10 birds. Cellulose and hemicellulose were significantly (p<0.05) reduced in the biodegraded PKC compared with the unbiodegraded PKC and PKC supplemented with Roxazyme G2G. The level of soluble sugars increased in a similar trend. Crude protein, phosphorus and energy increased significantly (p<0.05) in the biodegraded PKC compared to that treated with Roxazyme G2G and the unbiodegraded PKC. Apparent digestibility of nutrients was significantly improved (p<0.05) in birds that received the diets based on the biodegraded PKC than those on the unbiodegraded PKC and Roxazyme G2G supplemented diets. Feed conversion and weight gain in birds were significantly (p<0.05) higher in birds on the diets based on the biodegraded PKC compared to those on diets based on the unbiodegraded PKC and Roxazyme supplemented diets. Results of the study showed that PKC can act as a substrate for the production of a multienzyme complex from the 4 fungi. The enzyme complexes so produced were more efficacious in breaking down the cellulose and hemicellulose in it compared to Roxazyme G2G which is an enzyme product specific for cereal-based diets.
  B.A. Adeniyi and O.O. Ayepola
  Extracts of leaves of Eucalyptus camaldulensis and Eucalyptus torelliana were screened phytochemically for the presence of secondary metabolites and for in vitro antibacterial properties. Methanol and dichloromethane extracts of leaves of Eucalyptus camaldulensis and Eucalyptus torelliana were studied for their antibacterial activity against 8 clinically isolated organisms of gastrointestinal origin viz., Klebsiella species UCH 2101, Proteus mirabilis UCH 2102, Proteus mirabilis UCH 2204, Salmonella typhi UCH 2201, Escherichia coli CHO 3101, Escherichia coli UCH 2103, Pseudomonas aeruginosa CHO 3102 and Pseudomonas aeruginosa UCH 2203. The result of the phytochemical screening showed that both extracts contained tannins, saponins, cardiac glycosides but in addition to these, E. torelliana was found to contain anthraquinones. Both extracts were also found to inhibit all the isolates at 10 mg mL-1 concentration. The diameter of zones of inhibition exhibited by the extracts was between 10 and 22 mm. The methanol extracts compared favorably with gentamycin used as a standard control. The minimum inhibitory concentrations determined by the agar dilution method were between 0.04 and 10 mg mL-1. The results obtained from this study reveals that extracts of Eucalyptus camaldulensis and Eucalyptus torelliana possess antibacterial activities against enteric pathogens and the extracts may be a potential source of new antimicrobials against enteric organisms.
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