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Articles by Ashraf A. Tabll
Total Records ( 3 ) for Ashraf A. Tabll
  Abdelfattah M. Attallah , Ashraf A. Tabll , Mohamed F. Ismail , Ashraf S. Ibrahim and Ibrahim El-Dosoky
  The changes in lymphocyte subsets from 50 patients with colon cancer (25 patients with grade I and 25 patients with grade II) and 50 normal individuals were determined using flow cytometry. Flow cytometric immunophenotyping by direct and indirect immunoflourescence method was conducted using monoclonal antibodies to CD3 (T-lymphocytes), CD4 (helper/inducer T-cells) and CD8 (suppressor/cytotoxic T-cells) cell surface markers. Immunophenotyping of patients with colon cancer showed a significant decrease (p<0.001) in blood T-lymphocytes CD3 and helper/inducer T-cells CD4. Percentage of CD8 T-cells in colon cancer patients increased but did not reach to a statistical significant. A difference in lymphocyte subsets (CD3 and CD4) in GI and GII with high significance p<0.001 was shown compared with the control. CD4/CD8 ratio for healthy controls was (1.44±0.88) and decreased in colon cancer (1.36±0.93) without significance differences, however the difference between GII colon cancer and the control was highly significance p<0.001. In conclusion, colon cancer may associate with Dysregulation of cellular immune response and that T-lymphocytes subsets may play a role in the immunopathogenesis of colon cancer.
  Ashraf A. Tabll and Mohamed El-Sadany
  Hepatitis C virus serotype analysis of selected specimens from patients with liver cirrhosis positive and negative for HCV RNA was determined. Twenty four serum specimens were evaluated and stored at -20°C. The specimens all tested positive for HCV antibodies on a screening enzyme immunoassay, with confirmation on a recombinant immunoblot assay (RIBA). All specimens were also tested for HCV RNA by the reverse transcription polymerase chain reaction (RT-PCR). The sera were submitted to ELISA, modified, for the identification of antibodies against HCV serotypes 1, 2, 3, 4, 5 and 6 (Murex HCV serotyping l-6 assay). Out of 24 serum samples, 3 samples were undetectable. Type 4 predominated (81%), followed by type 2 (19%), types 1, 3, 5 and 6 were did not detected in the tested samples. Mixed HCV serotype 4 and 2 were detected in 3 patients (14.2%). All positive HCV antibodies sera were also positive by RIBA test. Of 21 serum samples positive for HCV antibodies, 13 samples (13/21, 61.9%) were positive for HCV RNA. HCV serotype 4 was found positive in 10/13 (76.9%) of positive HCV RNA. In negative HCV RNA serum samples (n = 8), HCV serotype 4 were found positive in 6/8 (75%). In conclusions, HCV Serotype 4 is the predominate type in the tested patients. Serotype 1, 3, 5 and 6 were not detected in the tested samples Analysis of HCV serotyping was possible in patients who had negative for HCV RNA from their serum.
  Maha A. El Demellawy , Abeer Abdel Wahab , Essam M. Emad , Kamal M. Kandeel , Ashraf A. Tabll and Mostafa K. El Awady
  This study was designed to compare microscopy, culture and 3 nested Polymerase Chain Reaction (PCR) based assays using as templates either of the followings: IS6110, mtp40 or 85B-RNA in the diagnosis and treatment follow up of pulmonary tuberculosis. Sputum specimens from 250 patients clinically diagnosed to have pulmonary tuberculosis were utilized. Samples were categorized into 4 groups. Group I: Samples from 120 patients with suspected TB infection; Group II: Samples from 70 patients relapsed after treatment, Group III: Samples from 30 patients not responding to treatment and Group IV: Samples from 30 patients subjected to follow up every two months during the treatment. The results of this study revealed that PCR is equally sensitive in all groups studied. TB DNA detection by PCR is more sensitive than ZN staining when taking culture plus clinical investigations as a gold standard method. In those patients with negative ZN, negative culture but have clinical and/or radiological evidence for the disease, PCR and RT-PCR methods were able to detect TB DNA and TB RNA at sensitivities of 96 and 100%, respectively. False positives were observed in TB DNA by IS6110 PCR at the end of successful treatment (probably due to detection of DNA from dead bacilli). On the contrary, RT-PCR of 85B-RNA is more specific and sensitive method for detection of viable mycobacteria. Present data altogether indicate that amplification of mtp40 and 85B-RNA are fast, sensitive and specific methods for diagnosis and follow-up of TB infection with slightly more specificity of 85B-RNA than mtp40 DNA.
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