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Articles by Ashok Kumar Tiwari
Total Records ( 3 ) for Ashok Kumar Tiwari
  Monika Kaul , Megha Kadam , Yashpal Malik , Ashok Kumar Tiwari , Jawaharlal Vegad and Bikash Chandra Sarkhel
  Avian Infectious bronchitis virus belongs to the family Coronaviridae. It is an enveloped virus with large positive stranded RNA genome. In the present study RNA was isolated from viral suspension and transcribed into cDNA. Poultry postmortem cases showing lesions of visceral gout were collected and infectious bronchitis virus were isolated. About 1.2 kb Nucleocapsid gene of virus was amplified by RT-PCR from four clinical samples. The amplified product was cloned and the nucleotide sequence of the N gene of an Indian field isolate was determined. The Indian IBV isolate exhibited 95% homology with Korean isolates and Chinese vaccine strains indicated conserved nature of N gene. Haemagglutination assay and chicken embryo inoculation was carried out for antigenic studies of the virus. The virus titre was confirmed using haemagglutination assay and IBVN2 showed the 1:2048+ titre. Propagation of virus was done by chorioallantoic method of inoculation of virus suspension in embryonated eggs. Characteristic curling and dwarfing of embryos was noticed in CAM inoculated embryonated eggs. Inoculated eggs showed teratogenic changes and deposition of urates as indication of naphropathogenic nature of virus.
  Ashok Kumar Tiwari , Iragavarapu Anusha , Maddineni Sumangali , Domati Anand Kumar , Kuncha Madhusudana and Sachin Bharat Agawane
  Aim: Increased consumption of sugar-sweetened beverages have been identified as one of the major sources responsible for increasing global epidemic of hyperglycemia, hyperglycemia induced oxidative stress and consequently, development of number of diabetic complications. This research explored preventive and therapeutic potentials of fruit juice of Benincasa hispida (BH) and Sechium edule (SE) in fructose and sucrose-sweetened beverages induced Impaired Glucose Tolerance (IGT), oxidative stress and IGT induced other metabolic disturbances in rats. Materials and Methods: Rats were orally administered fructose and sucrose sweetened-beverage prepared in water and respective fruit’s juice for one month. A group of rats were maintained for three months on daily single dose of sucrose. Three months sucrose-fed rats were later treated with fruit’s juice for fifteen days. Degree of IGT development was determined following Oral Glucose Tolerance Test (OGTT). Shape of plasma glucose concentration curve following OGTT, glycemic load, plasma Total Antioxidant Potential (TAOP), oxidative stress status, platelet aggregation activity and, Advanced Glycation End-products (AGEs) level in blood of rats were examined. Results: Fructose was more potent in inducing IGT than sucrose. Sucrose was more potent in decreasing plasma TAOP and inducing oxidative stress than fructose. BH juice prevented development of IGT induced by fructose and also significantly (p<0.05) reduced two hour glycemic load following OGTT. Three months continuous single dose administration of sucrose in rats induced development of IGT, increased two hour glycemic load (p<0.05) following OGTT, decreased TAOP (p<0.05), increased oxidative stress (more than twice) when compared with normal rats. Treatment of theses rats with juice of BH and SE accelerated normalization of oral glucose tolerance ability and decreased oxidative stress, platelet aggregation and accumulation of AGEs in sucrose induced IGT and oxidatively stressed rat. Conclusion: Our observations reveal that consumption of BH and SE juice may help reduce development of hyperglycemia and hyperglycemia induced development of complications by virtue of their antihyperglycemic and antioxidative stress potentials.
  Ashok Kumar Tiwari , Domati Anand Kumar , Pisupati Sri Rajeshwari Sweeya , Kondepudi Manikanta Abhinay , Anusha Chauhan Hanumantha , Vadala Lavanya , Ambati Sudharshana Raju , Kayyuru Sireesha and Konda Pavithra
  Aim: Insulin resistance and leptin resistance are hallmarks in common for development of type 2 diabetes mellitus (T2DM) and obesity. Both these conditions are associated with increased activity and expression of Protein-Tyrosine Phosphatase 1β (PTP 1β). Therefore, inhibition of PTP 1β activity or down-regulation of its expression has become an attractive area of therapeutics development in order to ameliorate phenomenon of insulin and leptin resistance. This research analysed and assessed PTP 1β inhibitory activity in thirteen easily available and commonly consumed vegetables’ juice. Materials and Methods: Percentage yield of the juice, total polyphenol and total protein concentrations were estimated in fresh juice of vegetables. PTP 1β inhibitory activity by vegetables’ juice in cytosolic supernatant of goat liver and skeletal muscle was determined. Results: Wide variations in juice yield, total polyphenol and protein concentration in vegetables’ juice were found. Juice of Ridge Gourd (RG) and Yellow Pumpkin (YP) were rich source of both, polyphenol and protein. Green Cucumber (GC), Bottle Gourd (BG), Snake Gourd (SG), RG, Radish (RD), Carrot (CT), Noolkol (NK) and Yellow Cucumber (YC) displayed reversible inhibition of PTP 1β. Juice of vegetables YP, Ivy Gourd (IG), Chayote (CH), Ash Gourd (AG) and Banana Stem (BS) displayed irreversible PTP 1β inhibition. Based on the order of PTP 1β inhibition potential in both liver and skeletal muscle, juice of RD and BG were identified most potent. Conclusion: This research finds vegetables’ juice as potent source of PTP 1β inhibitor and may offer cost effective therapeutic potential in reducing risk of insulin resistance and development of T2DM.
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