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Articles by Asha Sinha
Total Records ( 5 ) for Asha Sinha
  Ravindra Kumar , Seweta Srivastava , Manisha Srivastava and Asha Sinha
  In present study, the influences of different organic soil amendments on soil fungi was studied. Soil organisms carry a wide range of processor that are important for soil health and partially in both natural and managed agricultural scales. The total number of organisms, the diversity of species and activity of soil biota will fluctuate as soil environment changes. Three types of soil amendments and fertilizers viz., urea, FYM and vermicompost were used to amend the cultivated agricultural soil. The fungi were isolated from soil by using dilution plate technique and soil plate method. The dynamics of soil fungi were observed qualitatively as well as quantitatively. The maximum number of fungi was recorded when soil amended with FYM (40.6x104 g-1), urea (38.8x104 g-1) of dry soil at different concentration 2.0, 1.5 and 2.0%, respectively. In control where soil was not amended with any organic amendment, the number of fungi was 13.0x104 to 16.8x104 g-1, 14.4x104 to 16.8x104 g-1, 13.8x104 to 16.8x104 g-1 in urea, FYM and Vermicompost, respectively. A total 25 fungi were observed during the experimental period. Eighteen were observed when soil amended with urea, twenty-two observed when soil amended with FYM and 20 when soil amended with vermicompost. In control soil only fifteen fungi were recorded. The result showed that the number of fungi was increased in amended soil. Qualitatively, the fungi Rhizopus stolonifer, Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Penicillium citrinum, Alternaria alternata and Curvularia lunata, White Sterile Mycelium and Black Sterile Mycelium were observed.
  Ravindra Kumar , Asha Sinha , Seweta Srivastava and Manisha Srivastava
  The aim of the present study was to determine the decomposition rate of Sesbania aculeata L. by CO2 evolution and to estimate Soluble Crude Protein (SCP) production by dominant decomposing mycoflora of Sesbania aculeata L. Eight dominant decomposing mycobiota were selected for the study. In the substrate induced respiration the significant difference was observed in both sterilized and unsterilized substrate with the test fungi. The maximum CO2 evolution was observed with Aspergillus niger in sterilized (16.04 μg day-1) and unsterilized green manure (18.92 μg day-1). In other experiment conducted for the estimation of soluble crude protein production Penicillium citrinum has produced maximum SCP (26.54%) at 25°C followed by Trichoderma harzianum, Aspergillus niger and Curvularia lunata whereas minimum soluble crude protein production was observed in Penicillium rubrum (8.46%) at 35°C. The maximum per cent biomass reduction observed by Aspergillus niger (28.60%) at 25°C and minimum was found in Penicillium rubrum (3.80%) at 35°C. Among seven different nitrogen sources tested against Penicillium citrinum, the highest producer of SCP, potassium nitrate was found to be the best for maximum SCP production (26.54%) whereas the least suitable nitrogen source for SCP production by Penicillium citrinum was recorded to be sodium nitrate (14.85%).
  Saurabh Singh , Asha Sinha and Richa Raaj
  Mungbean seeds genotype HUM-4 and HUM-12 were infested with four dominant fungi viz., Alternaria alternata, Aspergillus niger and Penicillium citrum and Fusarium moniliformae as well as fresh seeds were used to test germination ability of mungbean seeds by using different methods viz., Blotter methods, Multi-pot trey methods, Earthen pot method and Rolled paper towel method. Result showed that all infested seeds were found to be significantly reduced the germination of seeds. In Blotter condition, maximum germination was recorded (93.30%) in genotype HUM-12 followed by (90.35%) in Genotype HUM-4. In paper towel methods, freshly harvested mungbean seeds showed maximum seed germination (90.00%) in genotype HUM-12 followed by (88.67%) in Genotype HUM-4. In multipot trey method, freshly harvested mungbean seed showed maximum seed germination (84.12%) in genotype HUM-12 followed by (83.33%) in Genotype HUM-4. In earthen pot methods freshly harvested mungbean seed showed maximum seed germination (87.30%) in genotype HUM-12 followed by (85.23%) in Genotype HUM-4. Aspergillus niger infested seeds showed lowest germination followed by Penicillium rubrum infested seeds.
  Seweta Srivastava , Ravindra Kumar and Asha Sinha
  Various parts of the Jatropha curcas plant are of medicinal value, its wood and fruit can be used for numerous purposes including fuel. In the present study the effectiveness of Jatropha curcas oil on inactivation of some mycoflora were determined. As a measure of testing the antimicrobial property of Jatropha curcas oil were subjected against six selected fungi viz. Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium chlamydosporum and Penicillium glabrum. Poisoned food technique was used to evaluate the antifungal effect of J. curcas oil. Two different concentrations of Jatropha oil i.e., 100 μL and 500 μL were mixed with potato dextrose agar (PDA) medium in Petri plates. Maximum radial growth was shown by control of Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger i.e., 90 mm (full growth on petri plate) followed by the control of Alternaria alternata i.e., 77.3 mm and minimum growth was shown by Penicillium glabrum i.e., 21 mm followed by Aspergillus niger i.e., 33 mm at 500 μL concentration of Jatropha oil. Maximum percent inhibition was shown by Penicillium glabrum i.e., 82.96% followed by Aspergillus niger i.e., 63.33% at 500 μL concentration of Jatropha oil and minimum percent inhibition was shown by Fusarium chlamydosporum i.e., 31.59% at 100 μL concentration of Jatropha oil. From this experiment it was concluded that Jatropha oil has promising antifungal effect on Penicillium glabrum and Aspergillus niger.
  S. Singh , B. Bikram , J. Mishra , P. Trivedi , Rai Ajay Kumar , S. M. Yadav and Asha Sinha
  To encounter the acute shortage of properly decomposed organic manure the present study was conducted for investigating the pattern of decomposition of the temple waste. Isolation of the fungi from decomposing temple waste and soil mixed with manure was done by direct observation method, damp chamber incubation method and dilution plate technique. Total 28 fungus were isolated and highest fungal population was recorded by dilution plate technique followed by damp chamber and then direct observation method and according to their occurrence they were divided into three categories (1) Dominant fungi which show 70% frequency, (2) Common fungi which shows less than 70% frequency and (3) Rare fungi were observed once or twice during isolation. The moisture content of the decomposing temple waste recorded maximum 29.65% at 15 days of intervals. Deuteromycotina fungi were recorded highest 64.28% in comparison to Zygomycotina (7.14%) and Ascomycotina (3.5%). In early stages of infection Mucor racemosus, Rhizopus nigricans Alternaria alternata, Fusarium spp. were found abundantly but in later stages of decomposition prevalence of Aspergillus niger, Aspergillus flavus, Aspergillus fumigates, Aspergillus candidus, Penicillium rubrum, Penicillium citrinum was recorded. During the process of decomposition the moisture content of the temple waste gradually decreases whereas changes in pH follow an erratic pattern due to the activity of above mentioned mycoflora throughout the process of biodegradation.
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