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Articles by Asawin Wanbanjob
Total Records ( 2 ) for Asawin Wanbanjob
  Thongchai Taechowisan , Nantiya Chuaychot , Srisakul Chanaphat , Asawin Wanbanjob and Yuemao Shen
  Some endophytic actinomycetes (120) were isolated from the roots of Alpinia galanga. Identification of these endophytes was based on their morphology and amino acid composition of the whole-cell extract. Most isolates were classified as Streptomyces sp. (82), with the remainder belonging to Nocardia sp. (11), Microbispora sp. (3) and Micromonospora sp. (2). Eight isolates were unclassified and 14 were lost during subculture. The strain identified as endophytic Streptomyces sp. Tc052 strongly inhibited test microorganisms. This endophyte was cultured, the agar was extracted with organic solvent and the extract was purified on a column of silica gel to give a major component, which was identified to be kaempferol, isoscutellarin, umbelliferone and cichoriin on the basis of spectroscopic data. These compounds together with the extract were tested for their antimicrobial activity against bacteria and yeast using micro-dilution methods for the determination of Minimum Inhibitory Concentrations (MIC) and Minimum Microbicidal Concentration (MMC). The MIC values obtained with the crude extract varied from 64-128 μg mL-1 against tested microorganisms. All the isolated compounds showed various activities.
  Thongchai Taechowisan , Asawin Wanbanjob , Pittaya Tuntiwachwuttikul , Yuemao Shen and Saisamorn Lumyong
  Different extracts of Streptomyces aureofaciens CMUAc130 culture were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, crude ethyl acetate and 10% methanol in ethyl acetate extracts exhibited fungistatic activity against Aspergillus flavus, Colletotrichum musae, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Sclerotium rolfsii. Both ethyl acetate extract and 10% methanol in ethyl acetate extract were highly effective on all tested fungi, with Minimum Inhibitory Concentration (MIC) values ranging from 0.25 to 50 and 10 to 100 mg mL-1, respectively. The major active ingredients from those extracts were purified by silica gel column chromatography and identified to be 5, 7, 4`-trimethoxy-4-phenylcoumarin (1), 4`-hydroxy-5,7-dimethoxy-4-phenylcoumarin (2), 3`-Hydroxy-5,7,4`-trimethoxy-4-phenylcoumarin (3), 5,7,3`,4`-Tetramethoxy-4-phenylcoumarin (4) and 4`-hydroxy-5,7,3`-trimethoxy-4-phenylcoumarin (5) by NMR and mass spectral data, respectively. Five compounds (1 to 5) had activity against F. oxysporum with MICs of 0.30, 1.00, 0.40, 10.00 and 20.00 mg mL-1, respectively. Compounds 1, 2 and 3 also showed a synergistic effect when combined in different concentrations, displaying four times less concentration to reach complete inhibition in the growth of F. oxysporum.
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