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Articles by Antonio Carlos Paulillo
Total Records ( 5 ) for Antonio Carlos Paulillo
  Elizabeth Santin , Antonio Carlos Paulillo , Alex Maiorka , Laura Satiko Okada Nakaghi , Marcos Macari1 , Ana Vitoria Fischer da Silva and Antonio Carlos Alessi
  A study was carried out to evaluate the efficacy of Saccharomyces cerevisiae (SC) as an inhibitor of aflatoxicosis in broilers. Clinic, pathologic, immune and productive aspects of broilers fed with aflatoxin in the diets were studied. The results showed that the feed intake and weight gain were negatively affected (P < 0.01) for aflatoxin in diet (1000 ppb). The SC (0, 20%) added to diet did not ameliorated the negatively aflatoxin effect at broiler weight gain, however the SC improve the feed conversion in this birds. The humoral immune response of broilers vaccinated against Newcastle virus disease (NDV) was reduced for aflatoxin in diet, as also the liver, kidneys and bursa of Fabricius were negatively affected for this toxin at diet. The SC did not improve the hemagglutination inhibiting antibody titers in birds fed with aflatoxin in diet, however improve the immune response of broilers at challenge with strain velogenic of NDV.
  Fabiana Silva Lima , Elizabeth Santin , Antonio Carlos Paulillo , Luciano Doretto Junior , Vera Maria Barbosa de Moraes , Nilce Maria Queiroz Gama and Ruben Pablo Schocken - Iturrino
  The objective of this study was to evaluate different programs of the vaccination against Newcastle disease in Japanese quails. Two hundred and eighty eight 5-week-old Japanese quails were distributed into six different vaccine programs: T1 - vaccinated with LaSota strain of Newcastle virus (NDV) via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV LaSota strain via intra-conjunctiva instillation; T2 - vaccinated with NDV B1 strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of NDV B1 strain via intra-conjunctiva instillation; T3 - vaccinated with NDV Ulster 2C strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of age with Ulster 2C strain; T4 - vaccinated with NDV VG-GA strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV VG-GA strain via intra-conjunctiva instillation; T5 - vaccinated with NDV LaSota strain, which was inactivated and emulsified in mineral oil, subcutaneous, at five weeks of age and were not revaccinated; and T6 - not vaccinated. At 17 and 25 weeks of age, all birds were challenged with a velogenic strain of NDV and a group of specific pathogen free (SPF) broilers was used as control of the virus. Five and 14 days after challenge, tracheal and cloacal swabs were collected from each bird for virus isolation. The quails from all experimental groups did not show any clinical sign of NDV, although 100% of SPF broilers that died after the challenge showed clinical signs of Newcastle disease. NDV isolation was possible in all SPF broilers and 5 and 14 days after challenge in the non-vaccinated group of quails (T6), suggesting that quails can be NDV carriers. In the vaccinated groups, NDV was not re-isolated, demonstrating the importance of vaccination to control virus dissemination by quails infected with NDV.
  Fabiana Silva Lima , Elizabeth Santin , Antonio Carlos Paulillo , Luciano Doretto Junior , Vera Maria Barbosa de Moraes and Ruben Pablo Schocken - Iturrino
  This study was carried out to clarify the real role that was played by the quail (Coturnix coturnix japonica) in the epidemiological plan, under the perspective as infection source of the Newcastle disease virus (NDV). For this, they used specific-pathogen-free birds (SPF) that were housed with inoculated quails with a pathogenic strain (velogenic viscerotropic), of NDV (DIE50=108,15/0.1 ml), by the ocular-nasal via. Each group was composed by 6 SPF birds and 12 Japanese quails. After 4, 14 and 20 days since the inoculation of the quails with NDV, 6 SPF birds were put together with each group of quails, so that there was a direct contact among the species. After 5, 14 and 20 days since the challenge of the quails with NDV, the samples were collected through the vent swabs of the quails for the viral isolation (pathogenic virus) in SPF embryos. There was no clinical disease in the quails inoculated with NDV. Therefore, there was viral isolation from 5 to 14 days after the challenge with the NDV, demonstrating, this way the state of carrier of the quail NDV, which happened until 14 days of the experimental infection with this pathogen. So, 100% of the SPF birds which housed with the NDV infected quail, after 5 (group 1) and 14 days (group 2), died from 4 to 7 days after the direct contact among species. This way, the transmission of the pathogenic virus of the quail was evident until 14 days of the experimental infection with this pathogen (NDV) for the SPF birds that housed together, and that calls the attention to the importance of the quail from the epidemiological point of view as potential source of infection of the NDV to commercial birds that housed with these specie or near this breeding.
  Marcia Nishizawa , Antonio Carlos Paulillo , Antonio Carlos Alessi , Aryana Duker Nunes , Josie Maria Campioni , Laura Satiko Okada Nakaghi , Luciano Doretto Junior and Fabiana Silva Lima
  This study aimed the characterization of the importance of vaccination against Newcastle disease in white Pekin duck (Anas platyrhynchos), and investigated the state of carrier of the virus in this species. There were used 120 Pekin ducks, distributed at random into 4 groups, vaccinated or not. At 60 days of age, all groups were challenged with a pathogenic virus (NDV) suspension, EID50 = 108,15/0,1mL. Cloacal and tracheal swabs were collected after 6, 14, 20 and 30 days post-challenge for viral isolation in SPF embryonated eggs. White Pekin duck of all the groups did not demonstrate symptoms of the Newcastle disease. They were refractory to the clinical disease with the NDV. In Pekin ducks from control group, the viral isolation was obtained from 20 up to 30 days after challenge. It was demonstrated therefore the state of carrier of NDV of the Pekin duck. In ducks from vaccinated groups, the viral isolation was null. It was also demonstrated therefore the importance of the vaccination in the suppression of the state of carrier of the NDV in white Pekin ducks.
  Elizabeth Moreira dos Santos Schmidt , Antonio Carlos Paulillo , Elizabeth Santin , Rosangela Locatelli Dittrich and Edson Goncalves de Oliveira
  The influences of sex and age on hematological parameters were investigated in the ring-necked pheasant. Statistical comparisons were made for differences in values between male and female, 30 adults (52 week old) and 20 juvenile birds (5 week old). A variety of blood parameters showed statistically significant age and sex related differences. Adult males had higher values for Packed Cell Volume (PCV) and juveniles lower values for PCV. Adult females had higher values for basophils, the heterophil/lymphocyte ratio and total plasma protein. Juvenile females had higher values for hemoglobin. Adults had greater White Blood Cells (WBC), heterophils, eosinophils and monocytes. Serum biochemical values (uric acid, gamma glutamyl transferase, protein, albumin and globulins) were determined in young (5 week old) ring necked pheasants. There were no differences in any of these serum parameters among juvenile males and females.
 
 
 
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