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Articles by Amer-Zareen
Total Records ( 8 ) for Amer-Zareen
  Amer-Zareen , M. Javed Zaki and Nazir Javed
  Aqueous ginger extract was tested against root knot nematode Meloidogyne javanica. In vitro studies, higher concentrations of extract (100% concentration) suppressed root knot egg hatching and caused juveniles mortality. Pasteuria endospore attachment was greater in 25% concentration extracts compared to those at 100 and 50% concentrations. Plant growth improved and disease severity was reduced when combined applications of the plant extract and P. penetrans were applied. In treatments receiving 25% extract and P. penetrans, 72% females were parasitized by the bacterial antagonist and 9 spores of bacteria per juvenile were found attached with nematode cuticle.
  Amer-Zareen , M. J. Zaki and A. Ghaffar
  Fungal cell free filtrates were used in two different doses, enhanced plant growth and root knot nematodes infection was reduced where high doses of filtrate (100% concentration) were applied, in all test fungal filtrates. Culture filtrates of Paecilomyces lilacinus and Verticillium chlamydosporium at 100 percent concentration showed significant reduction in Meloidogyne javanica root knot infection on okra and broad bean as compared to Trichoderma harzianum, T. koningii, T. viride, Aspergillus restrictus and Aspergillus sp., which found less effective.
  Shabbar Hussain , Amer-Zareen , M. Javed Zaki and M. Abid
  All test cultivars of chickpea respondedto root knot nematode M. javanica. Plant tops in nematode inoculated pots were significantly hampered (at p<0.01) compared to nematode free control. Maximum suppression in plant height by nematode was observed in cv. Nes 95 0174 followed by cv. 91 A039, whereas maximum suppression in shoot weight was observed in cv. Nes 95 0012 & Nes 95 0174 in response to nematode infection. Lower degree of gall formation per root system was observed in cv. Nes 95 004. Whereas cv. 90 122, 93 127 & Nes 950174 limited nematodedensitiesin soil and root compared to rest of cultivars. According to the host response index (0- >10), all test cultivars were moderately resistant. Reproduction factor ( r) was higher in cv. Nes 96 003 (r =1.88) compared to rest ofcultivars. In biocontrol experiment CF of A. nigerw as effectively increased plant height, fresh shoot weight and root length followed by A. flavus and A. temarii compared to other treatments and control. Maximum reduction in nematodesoil density was achieved by A.flavus (>17%)followed by A. temarii (134%) compared to other treated pots. Gall formation per root system was significantly hampered (at p<0.001) where A. niger was applied followed by A. nidulans> A. flavus> A. terreus> A. fumigatus compared to control.
  Amer-Zareen , Nargis Jamil Khan and M. Javed Zaki
  In a greenhouse study P. lilacinus, T. harzianum and T. flavus were used as seed treatment and soil drench. Seed treatment by P. lilacinus reduced gall formation, egg mass production, nematode soil and root densities as compared to control and other treatments. P. lilacinus improved plant growth followed by T. flavus and T. harzianum comparing with control. In another experiment, where soil was treated with conidial suspension, maximum plant height and shoot weight was achieved by P. lilacinus > T. harzianum compared to control, whereas T. flavus was found less effective in the enhancement of plant tops. Maximum suppression in gall formation (at p <0.01) and egg mass production (at p<0.001) was obtained in okra plants treated with P. lilacinus whereas T. flavus and T. harzianum were almost equally effective. Conidial suspension of microbial antagonists used as soil amendment also reduced nematode root invasion (at p<0.001) as well as soil densities
  Amer-Zareen , M. Javed Zaki and Nargis Jamil Khan
  Seven species of Aspergillus were screened for their biocontrol potential against Meloidogyne javanica root-knot nematodes on tomato under greenhouse conditions. Culture filtrates (CF) of Aspergillus niger, A. terreus and A. tamarii improved plant height, root length and fresh shoot weight when used as soil drench or bare root dipped seedlings. Besides, maximum reduction in gall formation and egg mass production was obtained by CF of A. niger, A. fumigatus and A. terreus in both soil drench and bare root dip seedlings compared to control. A. niger was found most successful in minimizing root and soil densities of root-knot nematodes in soil drench treatments compared to other test CF and control. Bare root dipped seedlings by A. nidulans CF reduced the root-knot development in tomato roots and in rhizosphere.
  Imran Ali Siddiqui , Amer-Zareen , S. Shahid Shaukat and M. Javed Zaki
  Efficacy of different species of rhizobia including, Rhizobium trifolii, R. phaseoli, R. meliloti, Bradyrhizobium japonicum and Bradyrhizobium sp., were tested in the control of Meloidogyne javanica, the root-knot nematode on Vigna mungo under laboratory, greenhouse and field conditions. Cell-free culture filtrates of the rhizobium species significantly reduced egg hatching and caused mortality of M. javanica larvae In vitro. In pot experiments and under field conditions, Bradyrhizobium sp., gave better biocontrol along with enhanced plant growth and nodulation.
  Imran A. Siddiqui , Amer-Zareen , M. Javed Zaki and S. Shahid Shaukat
  The efficacy of Trichoderma viride, T. harzianum, T. hamatum, T. koningii and T. pseudokoningii was tested for the control of Meloidogyne javanica, root knot nematode in okra and mungbean. Culture filtrates of Trichoderma spp., significantly reduced egg hatching and showed nematicidal activity by killing second stage juveniles of M. javanica. Soil application with conidial suspension of T. harzianum significantly reduced nematode population densities and root knot development in okra and mungbean. Apart from suppressing root knot nematode, T. harzianum also elevated plant height and fresh shoot weight of both okra and munhbean.
  Amer-Zareen , Imran Ali Siddiqui and M. Javed Zaki
  A total of 28 species belonging to 16 genera of fungi parasitic on root-knot nematodes were isolated and identified. Aspergillus terreus, A. nidulans, A. tamarri and Fusarium anthophellum are appeared to be new report on root-knot nematode and not reported hitherto. Of the fungi isolated, Aspergillus flavus, A. fumigatus, A. niger, Aspergillus sp., Acremonium butyri, Alternaria alternata, Catenaria sp. Cephalosporium sp., Cladosporium cladosporoides, Cladosporium sp., Cunninghamella elegance, Curvularia lunata, Fusarium exosporium, Fusarium sp., Ulocladium atrum and a sterile fungus are new records on root-knot nematodes in Pakistan. Natural infestation of Paecilomyces lilacinus and Arthrobotrys sp. is also recorded first time from Pakistan.
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