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Articles by Alina Wagiran
Total Records ( 2 ) for Alina Wagiran
  Bashir Mohammed Abubakar , Faezah Mohd Salleh and Alina Wagiran
  Eurycoma longifolia which is known as Tongkat Ali is commonly found in Asian countries such as Malaysia, Indonesia, Thailand, Myanmar and Cambodia. This plant is famously known for its various pharmacological activities. The plant is also reported to consist of various types of important bioactive compounds such as quassinoids, canthine-6-one alkaloids, triterpenes, squalene derivatives, $-carboline alkaloids etc which are mostly found in the root part. The presence of these important phytochemicals contributes to their different types of therapeutic effects more especially in terms of aphrodisiac properties which have resulted in a massive increase in demand and production of their Herbal Medicinal Products (HMP). These situations have resulted in the production of E. longifolia HMPs whose quality are questionable, which might be as a result of restricted of sources that might lead to some unethical activities carried out by suppliers and manufacturers in order to gain more profit. Therefore, this review focused on adulteration issues such as contamination and substitution of E. longifolia HMP. The review also includes the possible solutions on how to improve the quality of these HMP so as they can be safe for consumption. Embracing pharmacovigilance in the preparation of the HMP, proper implementation of agricultural practices such as Good Agricultural and Collection Practices (GACP) and Good Manufacturing Practices (GMP) together with the establishment of effective regulatory bodies would undoubtedly improve the quality of E. longifolia HMP sold in the market. The detailed knowledge about the main composition of the E. longifolia HMP will help to ascertain their quality, efficacy and safety as these are very important toward quality control.
  Alina Wagiran , Ismanizan Ismail , Che Radziah Che Mohd Zain and Ruslan Abdullah
  Improvement on plant regeneration system from suspension cells culture of different japonica rice cultivar was attempted in the present study. The present study shows that callus induction varied depend on genotype. Nipponbare, Hayahishiki and Fujisaka 5 variety showed 100, 93 and 53% of callus induction respectively on LS medium supplemented with 2,4-D after 4 weeks in culture. Callus proliferation was observed at the scutellum and yellowish in colour. Suspension cell culture was initiated from 4 weeks old callus in N6 liquid medium contained with 3 mg L-1 2,4-D and 1 mg L-1 kinetin for one month with regular subculture. The embryogenic cells were characterized with 1% (w/v) Evans Blue. Primary study shows that shoot regeneration was highest on Nipponbare at 60%, while Hayahishiki and Fujisaka were at 30 and 20%, respectively when culture on MS basal medium contained 3 mg L-1 Kinetin and 0.5 mg L-1 NAA (SRM3). After 6 weeks, the callus covered with green shoot bud and later formed shoot with an average of 3-8 shoot per callus. Increase of shoot regeneration was observed when callus treated with partial desiccation in all variety tested. The regeneration frequency was highest at 76, 70 and 33% when Nipponbare, Hayahishiki and Fujisaka 5, respectively were treated with 48 h desiccation. However, shoot multiplication was low in 0 h desiccation ranging from 10-16%. After 8 weeks in optimal SRM medium, the plantlets was hardened in growth chamber for 2 weeks and later transferred into soil with 100% survival in all variety. The present study shows that rice regeneration system from suspension cells can be improved with modification of plant growth regulators and desiccation.
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