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Articles by Ahmed I. Youssef
Total Records ( 2 ) for Ahmed I. Youssef
  Ahmed M. Hassan , Ahmed I. Youssef and Pochana G. Reddy
  Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard for humans consuming contaminated poultry meat. The aim of this study was to determine OTA levels in the feed used in some poultry farms in Ismailia, Egypt and in organs of birds from those farms. Commercial ELISA kits were used to detect levels of OTA in poultry feed samples and main feed ingredients as well as its residues in bird’s tissues (Kidney, liver and muscles). Poultry feed samples were also tested for mycological contaminations. The most common commercial toxin binders were also evaluated in vitro. OTA concentration in poultry feed and ingredients ranged from 7.10-20.72 μg/kg. Soybean meal samples showed the lowest concentration, while rations formulated within the farm had the highest mean concentration. Producers using different toxin binders had a significant lower OTA concentration in their rations (p<0.05) as compared to those not using binders. OTA residues in different tissues were high in kidney and liver as compared to muscles. Asprigillus flavus was isolated from 80% of the examined feed samples. Different binders showed different abilities to bind OTA in vitro with highest binding capacity for yeast as compared to other commercially available binders.
  Dalia M. Hamed and Ahmed I. Youssef
  Staphylococcosis caused by methicillin-resistant Staphylococcus aureus (MRSA) infection in rabbitry is neglected as a cause of rabbit production impairment and zoonoses. This study aimed to monitor a rabbit flock problem associated with MRSA infection by following a rapid and simple sampling and diagnostic scheme. Resistance to antibiotics and zoonotic risk posed to human contact and food contamination were assessed. The identification of S. aureus was carried out by traditional bacteriological methods that confirmed with PCR. Results showed that a total of 80 (39.21%) out of 204 animals from apparently healthy rabbit were MRSA positive. The isolation rate was highest from nose/eye (26.47%), followed by skin affections (8.82%) and vaginal/perineum (3.92%) sampling sites. In post-mortem examination, MRSA was positive in 55 (26.96%) out of 204 animals. Isolation rate from lung was (22.05%) that was higher than from uterus (7.81%). Resistance to antibiotics was shown in 59.9% of the isolates. All tested isolates were methicillin and oxacillin resistant strains. Vancomycin and oxytetracycline also were resistant in 91.66% of strains. Ciprofloxacin is considered the drug of choice for treating multidrug resistant MRSA infections. MRSA was isolated from nasal swab of attendant and slaughterhouse workers (42.6%). In conclusion, high mortalities and infertility of rabbits caused by multi-drug resistant strains of MRSA with dissemination to environment and contamination of rabbit meat shed the light on its impacts on rabbit production and public health. Thus, large-scale epidemiological investigations of MRSA in rabbitry in Egypt are needed.
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