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Articles by Ahmad Majd
Total Records ( 4 ) for Ahmad Majd
  Homa Mahmoodzadeh , Ahmad Majd and Mehrdad Lahouti
  In the present research structure and ultrastructure of shoot apical meristem of canola (Brassica napus cv. Symbol) under salinity conditions were investigated. The experiments were conducted in five groups (0, 3, 6, 9, 12 dS m-1) under greenhouse conditions. Sampling of apical meristem and TEM tissue preparation procedure were carried out. Semithin and ultrathin sections were prepared and viewed in light and electron microscopy, respectively. The results included reduction of meristem size, disorders in meristem structure. Also formation of autophagic vacuoles was observed that is probably one of the plant responses to salt stress for more water storage in these vacuoles and decreasing of cell water requirements.
  Akbar Safipoor Afshar , Amir Mousavi , Ahmad Majd , Renu and Gerhard Adam
  The contamination of mycotoxins associated with head blight of wheat and other grains caused by Fusarium graminearum is chronic threat to crop, human and animal health throughout the world. Deoxinevalenol (DON), produced by the fungus, belonging to class trichothecene is believed to act as a virulence factor in fungal pathogenesis by inhibiting eukaryotic protein synthesis, thereby blocking or delaying the expression of defense related proteins induced by host plant. The putative site of action of DON is 60s ribosomal protein L3 (RPL3). In order to reduce the effects of DON in the host plants, we modified tomato RPL3 (LeRPL3) to introduce W258R/H259Y mutations so that amino acid residue 258 is changed from tryptophan to arginine and 259 from histidine to tyrosine. Transgenic tobacco plants expressing these modified LeRPL3 cDNAs were tested for growth pattern of T1 seedlings in presence of DON. When seedling of these transgenic tobacco plants were compared for growth in the presence of DON, a significant difference in growth rate and the ability to undergo differentiation was observed among those plants expressing the modified version of the Rpl3 gene, compared to those expressing the wild-type Rpl3 gene. Expression of the tagged gene product indicates that is was not due to somaclonal variation. These results indicate a possible mechanism of host plant resistance to the fungal pathogen F. graminearum among the susceptible cereal species based on the expression of modified Rpl3 genes.
  Shiva Talebi , Ahmad Majd , Masoumeh Mirzai , Sayeh Jafari and Masoumeh Abedini
  Potato with scientific name of Solanum tuberosum L., belongs to the Solanaceae family and is an edible herbaceous plant which has medicinal properties. In this study the process of anther and pollen development in the potato was studed. Flowers and buds are harvested at different stages of development, fixed in FAA and were maintained in 70% alcohol. The samples, being prepared and embedded in paraffin were cross-sectioned with the microtome. Staining was performed by haematoxylin and eosin. The results showed that the plant has 4 bags of pollen per each anther, anther wall development in this plant is of the kind found in the dicotyledonous plants and is formed of a layer of epidermis, a endotheciumlayer, middle layer and a feeder layer (Tatetum). Its nourishing layer is secretory and simultaneous cytokinesis is used to separate tetrad. The microspore tetrads are of quadrilateral (tetrahedral) type. Pollen grains include 2 cells at the time they are released and have three vegetative splits.
  Simin Nabizadeh , Ahmad Majd , Sadigheh Arbabiyan , Masoumeh Mirzai and Fariba Sharifnia
  Electromagnetic fields is one of the effective factors on the living organisms and therefore in the present study the effects of different treatments of electromagnetic fields on the growth indices, chromosomal state and pollen structures of pumpkin were assayed. Seed samples of pumpkin were treated by 2 mT of electromagnetic fields in 15 and 30 min, in two different groups named dry and wet. The results showed that the treatment affected some indices in the negative or somehow positive way. The root length and germination percentage however were not affected significantly. The hypocotyl length of 15 min treatment was decreased in comparison with control samples. The shoot length of wet treated samples was significantly reduced compare to controls. Caryologic studies showed that the most important effect of electromagnetic fields, belongs to chromosomal aberrations so that some mitotic phase such as metaphase and anaphase were delayed. SEM microscoping assay showed that more electromagnetic fields causes disorder of pollen surface.
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