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Articles by Agripina SAPCALIU
Total Records ( 2 ) for Agripina SAPCALIU
  Agripina SAPCALIU , Crenguţa PAVEL , Vasilica SAVU , Eliza CAUIA , Mioara MATEI and Ion RADOI
  The study presents changes of biochemical parameters and cytological profile in the haemolymph of Apis mellifera Carpathica bee in stressful conditions compared with aspects in haemolymph of natural living bees. Investigations were made in 2009 on a total of 20 colonies under stress factors and 176 colonies of healthy bees. Harvesting and processing of haemolymph fresh samples from both groups were followed by analysis via classical biochemistry and cytological examination. In stressed bees haemolymph were found significant increases in alkaline phosphatase (ALP), aspartataminotransferaze (AST), gammaglutamiltranspeptidaze (GGT), creatinine (Cre), total calcium (Ca), triglycerides (TG) and significant reduction of magnesium (Mg), compared with values in natural living bees. The others analyzed biochemical parameters (GPT/ALT, CPK, GLU, T-Pro, UA, Urea) showed no significant differences between stressed bees and natural living bees. Monitoring the metabolic status of bees in stress conditions requires only evaluation of a few biochemical parameters (ALP, AST, GGT, total Ca, Mg, Cre and TG). The cytological investigation showed differences in the proportion of haemocyte types between the 2 groups of natural living honeybees and the 2 groups of bees kept in stressful conditions. It was observed an inversion of proportion between plasmatocyte cells (PL) and granulocyte cells (GR), with increased percent of PL and prohaemocyte cells (PRO) in the haemolymph of stressed bees, wile oeonocyte (OE) and other cells showed no important variations. The cytological profile of bees in stressful conditions shows the importance of monitoring the differentiate haemocyte count. Biochemical investigations correlated to the cytological ones more accurately reflect the transformations that occur under the action of stressors in the bee haemolymph.
  Crenguta I. I. PAVEL , Liviu Al. MARGHITAS , Daniel S. DEZMIREAN , Otilia BOBIS , Lavinia BARNUTIU and Agripina SAPCALIU
  Royal jelly is a secretion product of the cephalic glands of nurse bees that is very sensible to the storage conditions. The aim of this study is to review the changes that occur in royal jelly composition during storage and possible freshness parameters that have been studied. Evaluation of coloration, viscosity, water-soluble protein fraction, enzyme glucose oxidase, furosine, 10-HDA was proposed to assess the RJ freshness. Actually, royal jelly is one of the most studied bee products, but further studies are necessary in order to establish an international standard of the product and its freshness parameters.
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