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Articles by Abdelmoumen TAOUTAOU
Total Records ( 7 ) for Abdelmoumen TAOUTAOU
  Erika BALAZS , Constantin BOTEZ , Abdelmoumen TAOUTAOU , Dana CURTICIU , Daniela BRICIU , Laura Cristina COTA and Alexandru BRICIU
  Cultivated potato is susceptible to many pests and pathogens, none of which is more of a threat to potato agriculture than the late blight disease, caused by the oomycete Phytophthora infestans (Mont.) de Bary. Resistant R genes from the wild species of Solanum demissum have been used by breeders to generate late-blight-resistant cultivars. In our research we tried to clone polymorphic bands that could be considered markers for resistant genes. We used 8 resistant potato varieties and one sensible form. After amplification of potato DNA with decamer primers, several polymorphic RAPD sequences were recuperated from the agarose gel, and cloned into E. coli bacteria using two cloning kits. The majority of cloned sequences had approximately the same size with the original recuperated sequence.
  Erika BALAZS , Constantin BOTEZ and Abdelmoumen TAOUTAOU
  Phytophtora infestans, the causal organism of late blight, is the most important fungal pathogen of potato. In the strategy of potato late blight control utilization of resistant variety is the most important. For creating new resistant varieties Marker Assisted Selection increase significant the efficiency of this process. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. We tested 8 primers for RB gene homologues from Solanum bulbocastanum on different potato species, including S. bulbocastanum, S. demissum, and S. tuberosum. Some primers amplified only S. bulbocastanum, and others amplified olso S. demissum and S. tuberosum.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL. , Erika BALAZS , Constantin BOTEZ , Adina CHIS and H. MATEI
  We used the 1D SDS-PAGE to study the compatible interaction P. infestans- Solanum tuberosum. A virulent isolate has been used to colonize the cultivars Bintje and Desiree and another genotype with the R2 resistance gene. Two proteins were induced in the case of bintje: 14kD parotein and metallocarboxypeptidase inhibitor. In the case of R2, three proteins were induced with two not identified. The two identified were: β-glucosidase and transcription activator PTI6.
  Abdelmoumen TAOUTAOU , Carmen SOCACIU , Doru PAMFIL , Erika BALAZS and Constantin BOTEZ
  Not available
  Daniela BRICIU , Doru PAMFIL , Alexandru BRICIU , Dana CURTICIU , Erika BALAZS , Abdelmoumen TAOUTAOU , Iulia POP and Laura COTA
  The polymorphic study of table grapes was performed on ten varieties provided from two resorts grapevine: S.D.E. Cluj and S.D.E. Iasi. To achieve this study on grape varieties we used RAPD method. Genetic diversity and relationships between individuals was evaluation on the basis of presence or absence of bands resulting specific genetic fingerprinting. In this purpose we tested a total of 22 RAPD primers only 12 were polymorph. We made two rehearsals for each primer, taking into account only visible bands presents in the two rehearsals. The fragments resulted by amplification with polymorph primers have a lengths between 200 and 1500 bp. Notation of bands has been made by reference to DNA marker with 1 the presence of bands and with 0 the absence of bands. On the basis of these results dendrogram was constructed.
  Daniela BRICIU , Doru PAMFIL , Alexandru BRICIU , Dana CURTICIU , Erika BALAZS , Abdelmoumen TAOUTAOU , Iulia POP and Laura COTA
  The wine sector is one of the economically most important agricultural activities in the world. An enormous diversity of Vitis vinifera L. varieties (cultivars) can be used in the production of wine, although only a small number is of commercial importance. Ten grapevine varieties have been analysed. Five varieties are used for red wine production and five for white wine production. Two types of samples were analyzed: young leaves and must. Leaves were collected at the S.D.E Cluj. Must was obtained right after grape crushing, containing the skin and the solid parts of the flesh. Three different methods were tested: Lodhi et al., (1994) modified by Pop et al., (2003); Faria et al., (2000); Sambrook et al., (1989). The first method was used for DNA extraction from leaves and also from must. Analyses with DNA extracted from leaves and monovarietal musts were performed using the following microsatellite loci: VVS2, VVS5, VVMD5, VVMD7, ssVrZAG 47, ssVrZAG 62 and ssVrZAG79. In DNA extraction methods we taken into account the fact that PCR is very sensitive to the presence of Taq polymerase inhibitors. Results showed that for must samples the second method was successful. In order to see if the allelic profile from the must is identical to the one from leaves it was charged in the same polyacrylamide gel PCR product from must and leaves. The size of microsatellite alleles obtained in must is in accordance with those from leaves. Extraction of DNA from leaves and must was achieved yielding SSR amplification. SSR markers could distinguish all varieties used in this study.
  Alexandru C. BRICIU , Doru PAMFIL , Ioan ZAGRAI , Daniela BRICIU , Ioana PETRICELE , Dana CURTICIU and Abdelmoumen TAOUTAOU
  Plum pox virus (PPV) is the causal agent of sharka disease, which is responsible for severe damage and important economic losses in the stone fruit industry. The disease mainly affects stone-fruit species, mainly apricot, plum and peach. Sharka is originated from Eastern Europe and was described for the first time around 1915 in Bulgaria (Atanasoff, 1932). In this study we collected thirty PPV isolates from one experimental orchard belonging to the Fruit Research and Development Station Cluj. Molecular strain differentiation was done with the help of RT-PCR technique by analyzing the genomic region (Cter)CP of the virus by using RFLP analysis we were able to distinguish the two major strains, D and M, based on Rsa I polymorphism located in the genomic region (Cter) CP.
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