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Articles by Abbas Mohamed Ahmed
Total Records ( 5 ) for Abbas Mohamed Ahmed
  Abbas Mohamed Ahmed , N.T. Mekki , Muna O. Elhaj and Sarah Mohamed Abusalab
  Forty batches of anthrax spore vaccine were produced by bioreactor and by Roux flask, 20 batches were obtained from each system. The number of doses produced in the flask system was found 10 times more than those obtained by the bioreactor. It is recommended to continue producing this vaccine by the Roux flask while research will go on to improve suitable conditions in the fermenter in order to obtain high doses and to avoid the drawbacks of the flask system.
  Abbas Mohamed Ahmed , A.M. Zakia , M.M. Mukhtar and A.M. El-Hussein
  Skin sections of sheep inoculated with live spores anthrax vaccine revealed edema, intense effusion of mononuclear cells and proliferation fibroblast in the dermis and subcutaneous tissues, whereas sections of skin of those inoculated with capripox vaccine showed hyperplasia and hydrophopic degeneration of epidermal epithelium and instance infilteration of cellular exudates in dermis and subcutaneous tissues. However, the lesions presented by the combined vaccine included the fore mentioned lesions.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nourand and M.A. Fadol
  An anthrax and sheep pox combined vaccine was made in a lyophilized form by mixing concentrated live spores of Bacillus anthracis (Sterne strain) of known count with sheep pox virus (0240 vaccine strain) of a known titer. It induced protection against anthrax in guinea pigs and sheep pox diseases in sheep after challenge with virulent Bacillus anthracis and sheep pox virus respectively. The vaccine was found stable after 15 month of preservation at -20C.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nour and M.A. Fadol
  The combined anthrax and sheep pox vaccine was prepared in a lyophilized form containing the recommended doses of both sheep and goat strain 0240 pox and anthrax live spore vaccine. Immunization of sheep with this vaccine revealed a considerable immune response when detected with immunocapture Enzyme Linked Immunosorbent Assay (ELISA), indirect ELISA and with (3-(4, 5-dimethylthiazolyl-2) -2, 5-diphenyltetrazolium bromide) (MTT) lymphocytes proliferation assay. The difference in titers of antibodies raised against capripox virus antigen was significant, it was also significant for antibodies raised against crude Protective Antigen (cPA) and killed spore antigen. Phytohemagglutinin (PHA) revealed high Stimulation Index (SI) before vaccination but low one after vaccination while the SPPV antigen showed slight SI before vaccination and high SI after vaccination indicating cellular immune response to this antigen. Low SI of PHA after vaccination might be due antimitogenic effect of SPV antigens.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ElHussein , Tageldin A.M. Nour and M.A. Fadol
  The immune response of Sudanese sheep vaccinated with capripox vaccine was detected by IFA, Ic-ELISA test and MTT lymphocytes proliferation assays. The difference between antibodies titers before and after vaccination was found significant when tested by IFA test and the difference between the mean OD values was also significant when Ic-ELISA was used. PHA was found more effective in stimulating peripheral blood lymphocytes before vaccination compared with the virus antigen. After vaccination, the mean stimulation index of the of the virus antigen was higher
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