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Articles by A.R. Bahaman
Total Records ( 4 ) for A.R. Bahaman
  O. Shahaza , S. Khairani-Bejo , Z. Zunita and A.R. Bahaman
  The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia.
  B.Y. Takele , S Khairani-Bejo , A.R. Bahaman and A.R. Omar
  Brucellosis poses a significant animal and public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the Brucella melitensis specific IS711 gene was developed and applied to mice clinical samples with experimental trial. Over an 8 week period of infection, whole blood and serum were examined from 78 experimental mice, with a total of 60 samples from B. melitensis infected mice and a group of 96 control samples from mice inoculated with Brucella abortus 544, Yersinia enterocolitica O:9 and Brucella broth. Regardless of date of infection, the sensitivity of whole blood and serum based PCR assay with samples from B. melitensis infected mice was found to be 100% (30/30) and 83.3% (25/30), respectively. Serum samples collected at 60 days post infection (p.i) of B. melitensis failed to show positive result. An amplicon of 252 bp was obtained in all PCR positive samples. All samples obtained from the control groups tested negative, conferring an assay specificity of 100%. These results show that though, use of serum-PCR may lead to assay simplification and shorten turnaround time, but the optimal clinical specimen for this test was not serum but whole blood, which leads to maximum assay sensitivity.
  I.M. Ahmed , S. Khairani-Bejo , L. Hassan , A.R. Bahaman and A.R. Omar
  The potential diagnostic ability of Recombinant Outer Membrane Proteins (rOMPs), a combination of equal concentrations of rOMP25, rOMP28 and rOMP31of Brucella melitensis was investigated using Enzyme-Linked Immunosorbent Assay (ELISA) to differentiate the False Positive Serological Reactions (FPSR) in the serological diagnosis of caprine brucellosis. The rOMPs was tested using sera from three groups of goats with known Brucella exposure status which represent, naturally B. melitensis infected goats (infected), Brucella free goats (non-infected) and goats vaccinated with B. melitensis Rev. 1 vaccine strain (vaccinated). Additionally, all the sera were tested using the common serological tests which are Rose Bengal Plate Test (RBPT), BRUCELISA-400SG and Complement Fixation Test (CFT). When testing infected and non infected groups, the rOMPs I-ELISA recorded 94.44% (34/36) sensitivity and 100% (36/36) specificity and this almost agreed with the results obtained from testing the same serum samples using RBPT, BRUCELISA-400SG and CFT. However, when goats vaccinated with B. melitensis Rev. 1 vaccine strain were tested by the common serological tests, RBPT, BRUCELISA-400SG and CFT they wrongly recorded positive results for all the tested serum samples (26/26). While the developed rOMPs I-ELISA was able to differentiate the vaccinated from infected animals with 94.44 sensitivity and 84.62% specificity. The potential diagnostic ability of rOMPs would be of great importance as serologic marker to minimize the FPSR in eradication programs of caprine brucellosis.
  S.N. Mohamed-Hassan , A.R. Bahaman , A.R. Mutalib and S. Khairani-Bejo
  Rats are considered as one of the most important sources of leptospirosis as they are present in abundance in many environments. They caused significant economic losses and served as reservoirs for many zoonotic diseases. One of the diseases is leptospirosis which is considered a re-emerging disease in Malaysia. However, knowledge of the epizootic of leptospirosis and leptospiral serovars associated with rats is lacking. The objectives of the study therefore were to determine the distribution of rat’s species and their carrier status. In Malaysia, R. tiomanicus was found to be the dominant species found in Malaysian environments and it constitutes 86.0% (420 out of 488 rats) of the rats caught in different types of localities; National Service Training Camps (Kelantan, Terengganu, Malacca and Selangor), oil palm estates (Terengganu and Malacca), Royal Belum Rainforest (Perak), Suburban areas (Kelantan and Perak) and PULAPOL (Negeri Sembilan). Sixty leptospiral isolates (12.3%) were successfully cultured from the kidneys of the rats caught. Polymerase Chain Reaction (PCR) assay revealed 42 (8.6%) of the isolates were pathogenic as disclosed by the 16S primers. Majority of the pathogenic leptospires were isolated from rats caught in the National Service Training Camps (NSTC). The high rate in NSTC posed a major threat to the trainees as they were frequently involved in outdoor activities and exposed to infected environment. Therefore, control of rat population is crucial in minimizing the risk of transmitting leptospirosis to human.
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