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Articles by A.O. Canama
Total Records ( 2 ) for A.O. Canama
  D. Ruswandi , A.L. Carpena , R.M. Lantican , D.M. Hautea , A.O. Canama and A.D. Raymundo
  Downy mildew caused by Peronosclerospora philippinensis (Weston) Shaw is one of the most persistent diseases encountered by maize producers in the Philippines. Improved durable resistant host plant could provide an effective way of both increasing and stabilizing corn production in affected areas. An extensive research to study the genetics of downy mildew resistance in maize against the UPLB isolate pathogen using generation mean analysis and to locate QTL conferring resistance against P. philippinensis causing Philippine downy mildew were done. The genetics of resistance to P. philippinensis was studied in progenies derived from crosses between the resistant inbred P 345 and Nei 9008 and susceptible inbred Pi 17 and Pi 23. Plant generations used in this study were the PS (susceptible lines); PR (resistant lines); F1, F2, F3, BS and BR. Analysis of generation means indicates that additive-dominance gene effect plays an important role in all components of resistance in all crosses involving Nei 9008. On the other hand, additive-dominance with epistatic gene effect plays an important role for all components of resistance involving P 345. QTL conferring resistance against P. philippinensis was observed in a BC1F2 population developed from a cross between Pi 23 and P 345. QTL analysis of the different components of resistance showed that a total of twenty-seven regions distributed in nine chromosomes of the maize genome were associated in varying degrees with resistance to P. philippinensis. Four regions located in chromosomes 1, 5, 6 and 8 were associated with disease incidence and disease severity. Results suggest that it may be possible to incorporate QTLs of various components of resistance into the elite inbred line Pi 23 using marker aided selection.
  D. Ruswandi , Agustian , E.P. Anggia , A.O. Canama , H. Marta , S. Ruswandi and E. Suryadi
  Drought stress is a very important factor in the reduction of maize production in Indonesia. A series of experiments were conducted to identify early maturing mutants, to study their genetic similarity and to select for mutants tolerant to drought stress. The first experiment was done in Jatinangor to identify early maturing second generation mutants (M2). The experiment was laid on augmented design with two replications in which fifteen M2 population groups with their nonmutant parentals were used as genetic materials. The second experiment was conducted in Biotechnology Laboratory, Institute of Plant Breeding, University of the Philippines at Los Banos to analyse DNA of 28 early maturing M2 using SSRs markers. The third experiment was conducted in Majalengka to select mutants tolerant to drought stress. The experiment was arranged in augmented design consisting of two replications of 18 nonmutant parental lines and 161 M3 which have been selected for early maturity. The last experiment was laboratory screening in the Plant Breeding Laboratory, University of Padjadjaran to confirm field drought tolerant mutants. The experiment was arranged in a Randomize Block Design (RBD) using 64 of M4 which showed early maturity. In the first experiment, there were 35 M2 that showed early maturity. Gamma irradiation was found to increase the phenotypic variation and diversity of plant height, ear weight, days to tasseling and days to harvesting. The SSR analysis was found to be a valuable DNA marker system to study genetic diversity of mutant and non-mutant lines. The mutant and non-mutant lines were clustered into two major cluster and seven sub-clusters based on a phylogenetic tree analysis using UPGMA. Based on the field screening for drought in the third experiment and confirmatory experiment in the laboratory, M3DR 18.8 and its progeny of M4DR 18.8.1 (selfing of 18.8) and M3DR 18.5 and its progeny, M4DR 18.5.1 (selfing of 18.5) are tolerant mutant lines as shown by their positive index of drought and their physiological response to water stress simulation using PEG. Those mutant lines could be considered for breeding program for tolerance to drought as an anticipation of global climate change.
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