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by
A.N. Mahar |
Total Records (
6 ) for
A.N. Mahar |
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A.N. Mahar
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M. Munir
and
K.B. Laghari
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Production and infectivity of Steinernema carpocapsae, S. abbasi, S. karii and S. riobravis (entomopathogenic nematodes) using larvae and pupae of diamondback moth were studied. Nematodes production of all species was determined by the number infective juveniles (IJs) established in DBM larvae and pupae using sand and filter paper bioassy. Maximum number of S. carpocapsae were produced at 25°C, however the production of other isolates was higher at 30°C. Infectivity tests carried out in sand media revealed that maximum IJs of S. carpocapsae were recovered from the DBM larvae and pupae at 25°C whereas other three isolates produced reasonable number of IJs at 30°C. Similarly, DBM pupae with cocoon produced higher number of IJs than without cocoon. The research indicated that application of nematodes with the knowledge of insect pest biology that represents a possible new strategy for controlling DBM larvae and pupae. |
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A.N. Mahar
,
M. Munir
and
A.Q. Mahar
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Broth culture of Xenorhabdus nematophila and Photorhabdus luminescens have been observed lethal to the nymphs of locust Schistocerca gregaria when injected into the abdomen, applied orally, mixed with bran or applied to the foliage of food plants as compared to broth alone (control). A hyperbolic relationship was observed between different bacterial application methods of cells and their metabolites and time intervals. Bacterial cells and their metabolites caused more or less similar damage to the locust but it is supposed that the insect death was probably due to the toxic metabolites present in the bacterial cells. These bacterial cells were also recovered from the abdominal haemocoele indicating that bacterial symbionts do have a free-living existence and can enter in the haemocoele in the absence of nematode vector. If these bacterial symbionts are to be used for insect control, X. nematophila would be the most appropriate as it has never been previously reported from clinical specimens. |
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A.N. Mahar
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D.A. Darban
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S.R. Gowen
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N.G.M. Hague
,
N.D. Jan
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M. Munir
and
A.Q. Mahar
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The bacterium from Pseudomonas putida from Steinernema abbasi and its metabolic secretions caused the mortality of the Galleria mellonella pupae. Experiments were conducted in sand and filter paper on time exposure, temperature, moisture, dose and time of penetration of bacterium in pupae and tested stored or dried toxic metabolites using G. mellonella pupae as a test target organism. Death of pupae was probably due to the toxic metabolites. Pseudomonas putida cells were recovered from the haemocoele when bacterial cells were applied to the G. mellonella pupae indicating that bacterial cells can enter the haemocoele in the absence of nematode vector. Penetration of bacterium was found rapidly after application on G. mellonella pupae. Pseudomonas putida or its toxic secretions can be used as a microbial control for insect control. The experimental results indicate that there is possibility of using P. putida and its toxic secretions as a biopesticide and can contribute in the development of new microbial and biological control against insect pests. |
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A.N. Mahar
,
D.A. Darban
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A.G. Lanjar
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M. Munir N.D.
,
Jan N.G.M Hague
and
S.R. Gowen
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Susceptibility of late instar vine weevil Otiorhynchus sulcatus larvae and pupae to four species entomopathogenic nematodes were tested. Bioassays on production and infectivity to larvae and pupae were compared for two steinernematids and two heterorhabditis such as Steinernema carpocapsae, S. feltiae, Heterorhabditis indica and H. bacteriophora. Nematodes production of all species was determined by the number infective juveniles (IJs) established in vine weevil larvae and pupae O. sulcatus using sand and filter paper bioassay. S. feltiae produced the maximum number in larvae and pupae at 20°C as compared to other nematodes but production of H. indica, was better at 25°C in larvae and pupae followed by H. bacteriophora, S. carpocapsae and Infectivity test of larvae and pupae was also done in sand media. Infective juveniles recovered from larvae and pupae when infected with S. feltiae produced maximum infective juveniles at 20°C temperatures than all other isolates. H. bacteriophora produced higher number of IJs in larvae and pupae than all other nematode isolates at 25°C. This paper indicates the application of nematodes with the knowledge of insect pest biology represents a possible new strategy for O. sulcatus larvae and pupae. |
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A.N. Mahar
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N.D. JAN
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Q.I. Chachar
,
G.S. Markhand
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M. Munir
and
A.Q. Mahar
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Production and infectivity to cabbage butterfly, Pieris brassicae larvae and pupae was compared to four entomopathogenic nematodes such as Steinernema carpocapsae, S. feltiae, Heterorhabditis indica and H. bacteriophora. Nematodes production of all species was determined by the number infective juveniles (IJs) established in cabbage butterfly larvae and pupae using sand and filter paper bioassay. S. carpocapsae produced the maximum number in larvae and pupae at 25°C as compared to other nematodes but production of H. indica, was better at 30°C in larvae and pupae followed by H. bacteriophora, S. carpocapsae and S. feltiae. Infectivity test of larvae and pupae was also done in sand media. Infective juveniles recovered from larvae and pupae when treated with S. carpocapsae produced maximum infective juveniles at 25°C temperatures than all other isolates. H. indica produced higher number of IJs in larvae and pupae than all other nematode isolates at 30°C. This research indicates the application of nematodes with the knowledge of insect pest biology represents a possible new strategy for controlling cabbage butterfly larvae and pupae. |
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A.N. Mahar
,
M. Munir
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S.R. Gowen
and
N.G.M. Hague
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The entomopathogenic bacterium, Photorhabdus luminescens and its metabolites were found lethal to the Galleria mellonella when applied in sand media. Bacterium penetrated quickly in the haemocoele as it got contact with insect body. It was also observed that the toxic metabolites caused more larval death than the bacterial cells. P. luminescens cells were recovered from the haemocoele when suspensions containing bacterial cells were applied to the G. mellonella indicating that bacterial symbionts do have a free-living existence and can enter the haemocoele in the absence of nematode vector. This bacterium or its toxic secretions can be used for insect control that can be important component of integrated pest management against different insect pests. |
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