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Articles by A.M.S. Abdul Majid
Total Records ( 7 ) for A.M.S. Abdul Majid
  A.F.A. Aisha , H.B. Sahib , K.M. Abu-Salah , Y. Darwis and A.M.S. Abdul Majid
  Cytotoxic and anti-angiogenic properties of n-hexane extract of Sandoricum koetjape stem bark were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on Human Umbilical Vein Endothelial Cell (HUVEC) and both colon cancer and normal cell lines; HCT-116, HT-29 and CCD-18CO. Rat aorta ring assay was used to study the anti-angiogenic properties of the extract. At 100 μg mL-1, the extract showed 94±5.5% inhibition of the outgrowth of the blood vessels from the rat aorta rings. The extract also showed a dose dependent growth inhibition of all tested cell lines, IC50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL-1, respectively. At 50 μg mL-1, the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies.
  A.F.A. Aisha , S.A. Alrokayan , K.M. Abu-Salah , Y. Darwis and A.M.S. Abdul Majid
  Both cytotoxic and apoptotic properties of the stem bark extract of Sandoricum koetjape were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on three breast cancer cell lines and one normal cell line; MCF-7, MDA-MB-231, T47D and MCF-10A, respectively. Caspase Glo 3/7 assay was used to study the apoptotic activity on MCF-7 as a model cell line. The n-hexane extract showed a dose dependent growth inhibition of all tested cell lines with IC50 values between 44 and 48 μg mL-1. At 100 μg mL-1, the extract induced apoptotic cell death of MCF-7 by inducing activity of the effector caspases 3 and 7. These results indicated that n-hexane extract of S. koetjape has cytotoxic and apoptotic properties on breast cancer cell lines making it a good candidate for further studies.
  H.B. Sahib , A.F. Aisha , M.F. Yam , M.Z. Asmawi , Z. Ismail , S.M. Salhimi , N.H. Othman and A.M.S. Abdul Majid
  Angiogenesis is a process by which new blood vessels are formed from the pre-existing blood vessel. Orthosiphon stamineus Benth. OS has been used as a medicinal herb for many centuries. Due to the presence of high level of anti-oxidants and phenolic content compounds in OS and the effect of anti-oxidants and phenolic compounds being anti-angiogenic, the perturbation of new blood vessels ability of OS was tested. Dry powdered leaves of the OS plant were extracted with Petroleum Ether (PE), chloroform (CE), methanol (ME) and water (WE) by using sequential cold maceration method. The ME of OS has the highest anti-angiogenic activity (93.28±1.24%) in the rat aortic assay followed by CE (85.55±1.64%), PE (51.54±4.12%) and WE (50.22±1.23%) in descending order of reactivity. The methanol extract was also found to have potent anti-oxidant activity in the1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. The IC50 value was measured to be 0.286 mg mL-1. The total phenolic content of 1 mg mL-1 of methanol extract was equal to 38.27%.
  H.B. Sahib , Z. Ismail , N.H. Othman and A.M.S. Abdul Majid
  Estrogen Receptor (ER+) antagonist, Tamoxifen (TMX), is widely used in the treatment of the hormone responsive breast cancer. However, the common occurrence of resistance after prolonged treatment of TMX hampers its effectiveness. Orthosiphon stamineus Benth. (OS) is a common herb found in South East Asia and is used traditionally to treat various types of ailments. The aim of this study was to determine whether the methanolic extract of Orthosiphon stamineus Benth. (MEOS), that had been proven in previous study to act as anti-angiogenic agents, enhance the anticancer efficacy of ER+ antagonists. In this study methanolic extract of (MEOS) was treated to MCF-7 hormone sensitive breast cancer cell line with the addition of TMX. MEOS showed no significant cytotoxic effect towards MCF-7 when used alone, however when combined with TMX, the anti proliferative activity of the combination increased five fold higher when compared to the anti-proliferative activity of singly treated TMX. The result suggests that MEOS synergistically enhance the activity of TMX against hormone responsive breast cancer cells in vitro and may prove to be useful for the treatment of metastatic breast cancer.
  A.F.A. Aisha , K.M. Abu-Salah , Y. Darwis and A.M.S. Abdul Majid
  Angiogenesis is essential for the growth and metastasis of most solid malignancies. Accordingly, tumor angiogenesis is an important pharmacological target for cancer prevention and treatment. This study was conducted to study antiangiogenic activity of some tropical plants. Seven plants were extracted successively with n-hexane and methanol to prepare 18 extracts and antiangiogenic properties of the extracts were studied by rat aorta ring assay. Nine extracts showed more than 50% inhibition of the blood vessels outgrowth from the primary tissue explants. The MTT assay was used to study the cytotoxic activities of extracts with more than 50% inhibition. The Human Umbilical Vein Endothelial Cells was used as a test cell line versus two human colon cancer cell lines HCT-116 and HT-29, two human breast cancer cell lines MCF-7 and T47D and one hepatocarcinoma cell line HepG-2. The selectivity index on Human Umbilical Vein Endothelial Cells was calculated. The selectivity index results indicated antiangiogenic activities of three plants Parkia speciosa, Syzygium campanulatum and Sandoricum koetjape. The results presented in this research article make these plants good candidates for further studies to purify the active compounds and to study in vivo antiangiogenic activity.
  M.J. Siddiqui , Z. Ismail , A.F.A. Aisha and A.M.S. Abdul Majid
  Catharanthus roseus (Apocynaceae), in Malaysia known as Kemuning cina, well known for being rich in alkaloids was investigated for its cytotoxic activity by using MTT assay against Human Colorectal Carcinoma Cell Line (HCT 116). The preliminary cytotoxicity study demonstrated dose independent cytotoxic activity of the methanol extract of C. roseus when screened against HCT-116 colorectal carcinoma cell line. n-hexane, chloroform and methanol fractions also showed dose independent cytotoxic activity with chloroform fraction showing the highest activity. Water fraction showed a minor cytotoxic activity, vindoline also showed some cytotoxic activity at 200 μg mL-1. Catharanthine showed the most promising activity while dose dependent cytotoxic activity of its IC50 value was found to be at 60 μg mL-1. Simple and facile method has been developed for the isolation of compounds catharanthine and vindoline from this plant.
  N.S. Muslim , K.W. Ng , A. Itam , Z.D. Nassa , Z. Ismail and A.M.S. Abdul Majid
  Strobilanthes crispus (Acanthaceae) is widely used for treatment of cancer in the South Asian region. In this study, validation of GC-TOF mass spectrophotometric methods for quantitative determination of phytoconstituents in methanolic and aqueous extracts of S. crispus was conducted. The cytotoxicity of standardised methanolic and aqueous extracts of S. crispus was assessed against a panel of human cancer cell lines, namely breast carcinoma (MCF7), colon carcinoma (HCT 116), hepatocellular carcinoma (Hep G2), non-small cell lung adenocarcinoma (NCI-H23) and human breast ductal carcinoma (T-47D) cells and one normal colonic fibroblast cell line (CCD-18Co). The cell proliferation assay was performed using tetrazolium (MTT) method. Furthermore, the inhibitory effect of the extracts on angiogenesis was evaluated using ex vivo rat aortic ring assay. Finally, the antioxidant properties of both extracts were studied using DPPH free radical, xanthine oxidase activity and β-carotene-linoleate model system. Aqueous extract was found to be nontoxic towards all cell lines used, while the methanolic extract exhibited cytotoxic response towards the T-47D and MCF7 cells. Both the extracts demonstrated detectable anti-angiogenic activity. The extracts displayed very strong inhibitory activity towards xanthine oxidase enzyme, however, they demonstrated moderate antioxidant properties, which is evidenced by the quenching of DPPH free radical and preventing the bleaching of β-carotene by linoleic acid.
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