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Articles by A.M. Hassan
Total Records ( 4 ) for A.M. Hassan
  A.M. Hanafy , H.A. Khalil , Omnia E. Kilany , Marwa A. Hassan , Mohamed S. Yusuf , Abdelazim Ibrahim , I.M. Fares , A.M. Hassan and P.G. Reddy
  The objective of this study was to determine the optimal level of an Oil Mixture (OM) supplementation in drinking water to enhance the performance of older Japanese quail. Five hundred forty Japanese quail, 40 weeks old, were randomly assigned to 4 experimental groups (90 female and 45 male/group) that received OM at 0, 0.5, 1 and 2 mL/L–1 of drinking water during the experimental period of 42 days. Egg production, egg quality, fertility and hatchability percentages were evaluated. Serum alanine aminotransferase (ALT), aspartate transferase (AST), total protein, albumin, urea, creatinine, total lipids, cholesterol and triglycerides were estimated. Livers were examined for histopathological changes. Results showed that birds received 0.5 mL/L–1 of OM had significantly improved in most studied traits compared to the other treated and control groups. Laying rate, fertility, hatchability percentage, yolk index, internal quality unit and ovarian yellow follicle number were superior in 0.5 group than the other experimental groups. In contrast, birds received 1 or 2 mL had significantly higher concentrations of ALT and AST than birds that received 0.5 mL or control birds. Serum chemistry analysis revealed no significant effects due to treatments on kidney functions. Histopathological findings revealed disruption of normal hepatic architecture in birds that received 1 or 2 mL of OM supplementation compared to 0.5 mL and control birds. Our findings suggest that 0.5 mL/L–1 of OM could be enough and useful in improving productive and physiological performance of laying Japanese quail.
  A.M. Hassan , H. May AbdelAzeem and P.G. Reddy
  Effects of water supplements, sodium bicarbonate (NaHCO3), potassium chloride (KCl) and acetic acid were evaluated on the performance and immune system of chronically heat-stressed broiler chicks. Two hundred day old broiler chicks were allotted to one of the four groups (n = 50); 1) control [no supplements], 2) NaHC03, 0.5%, 3) KCl, 0.15% and 4) acetic acid, 1.5 mL/Liter. All chicks were kept in a controlled environmental chamber maintained at 33±2oC from day one to 6 weeks (wks) of age. Significantly higher weight gains coincided with decreased feed conversion ratios for all the supplemented groups as compared to control group at 2, 4 and 6 wks of age. Bursal index, percentage weights of thymus and spleen in relation to body weight and natural agglutinin levels, an indicator of humoral immunity were higher but the heterophil:lymphocyte ratio, an indicator of stress was lower for the supplemented groups as compared to control group. Total aerobic spore formers and Enterobactraecae counts in the intestinal swab samples were higher in control group than supplemented groups. Intestinal pH was lower at 2, 4 and 6 weeks of age but water consumption at 5 and 6 wks of age tended to be higher in acetic acid treated group as compared to other groups. Overall, the results indicated significant improvement in the performance and immune response of chronically heat-stressed broiler chicks given the water supplements, acetic acid being slightly superior to NaHCO3 and KCl.
  M.K. Gafar , A.U. Itodo , F.A. Atiku , A.M. Hassan and I.J. Peni
  Folliage of Hairy indigo (Indigofera astragalina) obtained from Sokoto state, Nigeria were studied for their proximate analysis and mineral composition. The proximate composition revealed the presence of moisture (51.00±0.50% fresh weight), ash (8.17±0.58% dry weight, DW), crude lipid (5.0±0.5% DW), crude fibre (2.67±0.29% DW), crude protein (8.23±0.11% DW) and carbohydrate (75.94±0.64%). The energy value was found to be 578.87 kcal/100 g. The minerals composition revealed, potassium (14.55±0.17 mg/100 g), sodium (0.33±0.16 mg/100 g), calcium (11.49±0.34 mg/100 g), magnesium (10.89±0.32 mg/100 g), phosphorus (0.39±0.01 mg/100 g), copper (0.02±0.00 mg/100 g), zinc (0.11±0.00 mg/100 g), iron (20.95±3.84 mg/100 g) and manganese (0.43±0.01 mg/100 g). These results revealed that the leaves of Hairy indigo (Indigofera astragalina) contained essential nutrients which compete favourably well with those of wild edible leaves in literatures.
  Jalil F. Mehrabadi , Q. Behzadian Nejad , Sh. Najar Peerayeh , M. Khodabandeh , H. Soleimanjahi and A.M. Hassan
  Uropathogenic Escherichia coli is one of the major agents of urinary tract infection. Since it has intracellular propagation, cellular immune response is so important in this case. Accordingly, a genetic construct for inducing of cellular immune system was designed. At first, chromosomal DNA extracted from E. coli 35218 and fimH gene amplified with this template by PCR. PCR product inserted to pcDNA.1 eukaryotic expression vector and confirmed the recombinant vector by sequencing. The COS7 cell line transfected with a complex of pcDNA/fimH and ExGen 500 poly cationic polymer. Expression of fimH gene in COS7 was confirmed by RT-PCR. Consequently, pcDNA/fimH cassette could express inserted fimH gene in eukaryotic cells and is a valuable DNA candidate cassette for urinary tract infection vaccination. This is the first prompt to designing a DNA vaccine against urinary tract infection that caused by Uropathogenic Escherichia coli.
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