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Articles by A.M. ELHussein
Total Records ( 4 ) for A.M. ELHussein
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nourand and M.A. Fadol
  An anthrax and sheep pox combined vaccine was made in a lyophilized form by mixing concentrated live spores of Bacillus anthracis (Sterne strain) of known count with sheep pox virus (0240 vaccine strain) of a known titer. It induced protection against anthrax in guinea pigs and sheep pox diseases in sheep after challenge with virulent Bacillus anthracis and sheep pox virus respectively. The vaccine was found stable after 15 month of preservation at -20C.
  A.Ballal , A.E. Karrar and A.M. ElHussein
  Serum samples were collected from commercial layer and broiler flocks 3 to 63 weeks old at four areas from Sudan. All flocks were not previously vaccinated against infectious bronchitis (IB) virus. Sera were tested using haemagglutination inhibition (HI) test for IB virus antibodies. 689(71%) out of the 957 samples examined had an HI titer ranging from 6 to 11 (log ). High incidence (90%) of IB virus infection was detected among layer flocks at Khartoum State and 46.8% of the samples had an HI titer > 10(log ). Low HI titer (<4 log ) was detected among samples collected from layer flocks at Sennar (Central Sudan). The results obtained indicated widespread and distribution of IB virus infection in Sudan.
  A. Ballal , A.E. Karrar and A.M. ElHussein
  Outbreaks of suspected IB disease occurring in a number of commercial layer flocks at Khartoum and El Gazira states were studied during years (2000- 2001). Affected birds were of different breeds (Lohmann, Bovans Hisex). Their ages were from 5 / –7 months. The main clinical signs varied from mild respiratory signs to diarrhea sometimes yellowish in colour. Drop in egg production (up to 60%) was recorded in some flocks. IB virus was isolated from these outbreaks. Using virus neutralization (VN) and haemagglutination inhibition (HI) tests, three of these IB field isolates (M114/2000, K170/2000 and K158/2001) were antigenically similar to IB virus strain 4/91 (Neutralization index 4.4 to 4.8 and VN titer > 11(log ) were recorded). Isolate (K110/2000) on the other hand, was antigenically similar to IB virus Mass. (M41) strain (HI titer >11 log was recorded). This 2 is the first time to identify IB virus strain antigenically similar to IB virus strain 4/91 in Sudan.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nour and M.A. Fadol
  The combined anthrax and sheep pox vaccine was prepared in a lyophilized form containing the recommended doses of both sheep and goat strain 0240 pox and anthrax live spore vaccine. Immunization of sheep with this vaccine revealed a considerable immune response when detected with immunocapture Enzyme Linked Immunosorbent Assay (ELISA), indirect ELISA and with (3-(4, 5-dimethylthiazolyl-2) -2, 5-diphenyltetrazolium bromide) (MTT) lymphocytes proliferation assay. The difference in titers of antibodies raised against capripox virus antigen was significant, it was also significant for antibodies raised against crude Protective Antigen (cPA) and killed spore antigen. Phytohemagglutinin (PHA) revealed high Stimulation Index (SI) before vaccination but low one after vaccination while the SPPV antigen showed slight SI before vaccination and high SI after vaccination indicating cellular immune response to this antigen. Low SI of PHA after vaccination might be due antimitogenic effect of SPV antigens.
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