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Articles by A.B. Abeer Mohammed
Total Records ( 2 ) for A.B. Abeer Mohammed
  Shehab E. Talat , Mahmoud Abd El-Mongy , Mona I. Mabrouk , A.B. Abeer Mohammed and Hanafy A. Hamza
  Background and Objective: β-lactam resistant bacteria are emerging highly drug-resistant causing infections accompanied with significant importance. This study was undertaken to detect the β-lactam antibiotic resistant P. aeruginosa obtained from edible foods in El-Giza governorate, Cairo, Egypt and role of the plant oils in reducing β-lactam resistance for P. aeruginosa . Fifty samples from edible food were collected from supermarkets. There are multi drug resistant bacteria presented in the tested edible food. Materials and Methods: Isolation were carried out using classical method by using selective medium then the isolates were identified using modern techniques by PCR and DNA sequencing techniques. Results: Results showed identified isolates were 50 pathogenic bacteria as Staphylococcus spp., 10 isolates (40%), Micrococcus spp., 1 isolate (4%), E. coli 11 isolates (44%), Citrobacter freundii 1 isolate (4%), Enterobacter species 1 isolate (4%), Enterobacter cloacae 1 isolate (4%) and P. aeruginosa 1 isolate (4%). The P. aeruginosa strain was resistance to piperacillin/tazobactam and meropenem, intermediate to gentamicin while sensitive to amikacin, levofloxacin and ciprofloxacin. Moreover, P. aeruginosa strain was sensitive to thyme oil but resistant to fennel, caraway and peppermint oils. Conclusions: Results concluded that treatment β-lactam resistant P. aeruginosa by aminoglycosides or fluoroquinolones antibiotics has significant value. Also, thyme oil considered one of the most important oils in the antimicrobial aspects. Finally treatment of resistant strain of P. aeruginosa by combination of antibiotic and medicenal plants even if resistant to these oils has significant potentiating effect.
  Mahmoud Abd El-Mongy , Ghada Mohammed Abd-El-Moneam , Amgad Ahmed Moawad and A.B. Abeer Mohammed
  Background and Objective: Escherichia coli (E. coli) strains causing systemic disease in poultry (avian colibacillosis) are termed avian pathogenic E. coli (APEC). Colibacillosis is a disease of severe economic significance to all poultry producers worldwide and is characterized by a diverse array of lesions. Escherichia coli that cause infections usually possess one or more virulence properties that may help in establishment of the infection. The aim of this study was to investigate the virulence genes in E. coli isolated from broiler chickens. Methodology: A total number of 125 chicken samples from apparently healthy broiler chickens (25 and 15), diseased broiler chickens (25 and 15) and freshly dead ones (25 and 20) were collected in winter (from December, 2014 to February, 2015) and summer (from June, 2015 to August, 2016), respectively from Kafr El-sheikh Governorate. Results: In winter season, E. coli was recovered from 43 broiler chickens with an incidence of 57.3% and the incidence of E. coli in apparently healthy broiler chickens was 32%, diseased broiler chickens 64% and in freshly dead ones 76% while in summer season E. coli was recovered from 21 broiler chickens with an incidence 42% represented 26.6% in apparently healthy, 40% in diseased chickens and 55% in freshly dead one. The serogroups of E. coli that obtained by serological identification were O78, O1, O26, O2, O127, O91 and O153. The results obtained by multiplex PCR reported that eaeA (intimin E. coli attaching and effacing) gene detected in O2, O26, O1 and O153, ompA (outer membrane protein) gene detected in all E. coli serogroups that isolated O2, O26, O78, O127, O1 and O91 except O153. Stx1 gene detected in O2, O26, O78 and O91. Stx2 gene detected in O78, O127 and O91. Conclusion: The present study showed a higher percentage of E. coli isolates carrying at least one virulence gene.
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