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In this study, the extract was tested for in vitro
activity against oxytetracycline-resistant V. harveyi. Toxicity
of the methanolic extract was evaluated by Brain Shrimp Lethality test.
Geodia sp. was characterized by three spicula types (oxeas, trianes
and oxyaster euaster), encrusting growth formation, hispid surface features
and skeletal structure of paratangential ectosome. The methanolic extract
of Geodia sp. exhibited anti-oxytetracycline-resistant V. harveyi
activity with MIC of 31.25 μg mL-1. The extract also was
able to inhibit the growth of oxytetracycline-resistant V. harveyi
in broth culture at concentrations of 1 and 2xMICs and able to kill almost
V. harveyi cells at 4xMIC. Interestingly, the extract did not show
any toxic effect in Artemia salina up to 125 μg mL-1.
It is the first report for the antibacterial activity of methanolic extract
of Geodia sp. against oxytetracycline-resistant V. harveyi,
a pathogenic bacterium in marine aquaculture. This results suggest that
Geodia sp. might be used as a source of alternative compound to
control marine bacterial pathogen especially oxytetracycline-resistant
V. harveyi.
