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by
A. Hansa |
Total Records (
2 ) for
A. Hansa |
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A. Hansa
,
R.B. Rai
,
M. Yaqoob Wani
and
K. Dhama
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Bovine coronavirus (BCoV) is widespread in cattle population, resulting in
heavy economic losses to both dairy and beef industry throughout the world.
The syndromes associated with BCoV include winter dysentery in adult dairy cattle
and respiratory and intestinal tract infections in young calves. The virus has
specific tropism for intestinal and pulmonary epithelial cells. Reports regarding
prevalence and molecular detection of BCoV from India are scarce. In this study,
101 fecal samples were collected from clinical cases of diarrheic calves from
North Indian region covering three dairy farms of Uttar Pradesh and dead calves
of post mortem facility of Indian Veterinary Research Institute. Fecal samples
from all the cases were screened for the presence of BCoV by commercially available
ELISA kit. Furthermore, all samples were subjected to RT-PCR for detection and
confirmation BCoV. RT-PCR was carried out using two different sets of primers
to amplify the conserved nucleocapsid (N) gene of the virus targeting a 407
and 730 bp fragments. An incidence rate of more than 14% (15/101) was observed
with ELISA and about 20% (20/101) by RT-PCR. The present report is first in
its nature regarding the detection of BCoV at molecular level in India. In conclusion,
RT-PCR was found more sensitive than commercial ELISA kit for detecting BCoV
in fecal samples. Further extensive epidemiological studies are suggested for
the virus in the country to know the magnitude of BCoV infection in dairy calves
along with isolation of viral strains and to investigate their antigenic and
genetic properties. |
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A. Hansa
,
R.B. Rai
,
K. Dhama
,
M.Y. Wani
,
M. Saminathan
and
G.J. Ranganath
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Bovine Coronavirus (BCoV) is widespread both in dairy and
beef cattle throughout the world. The virus is one of the largest RNA virus
and has specific tropism for intestinal and pulmonary epithelial cells. It is
responsible for huge economic losses by causing winter dysentery in adult dairy
cattle and respiratory and intestinal tract infections leading to pneumo-enteritis
in young calves. Isolation of BCoV has been reported to be difficult. Studies
regarding epidemiology, virus isolation and molecular detection from India are
very few. In the present study Vero cell line was used for isolation of the
BCoV from Enzyme Linked Immunosorbent Assay (ELISA) positive samples. Direct
florescent antibody technique (dFAT) and reverse transcriptase-polymerase chain
reaction (RT-PCR) were used to confirm the isolated virus strains at antigenic
and genomic levels, respectively. Out of the 15 positive fecal samples, virus
from only seven was able to infect vero cell line. Subsequently BCoV got adapted
to the vero cell line upto three passages, which was confirmed both at genomic
and antigenic levels by dFAT and RT-PCR testing. It can be concluded that vero
cell line can be used for isolation of BCoV, however due to the enormous stain
diversity of the virus it is possible that many stains can’t grow and get
adapt in this cell line. Further studies are required for isolation of different
viral strains, finding the susceptible cell lines and also to confirm the variations
among the BCoV isolates at antigenic/genomic levels. |
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