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Articles by A. Amid
Total Records ( 4 ) for A. Amid
  A. Amid and P. Jamal
  This research reports the study that aims to enhance the activity of xanthine oxidase inhibitor, a secondary metabolite, found in Chrysanthemum indicum and to identify the most suitable elicitors with the optimize elicitation time and concentration. Many plant species are well known source of secondary metabolite with a variety of biological activities. Production of biologically active compounds using plant cell suspension culture approach, normally gives low result. Optimization studies to enhance xanthine oxidase inhibitor activity using different elicitor concentrations for three different types of elicitor (chitosan, yeast extract and Aspergilus niger) were investigated. A factorial design was used from MINITAB software for optimization of the process. Analysis of results proved that chitosan is the best elicitor to increase activity of xanthine oxidase inhibitor in C. indicum to 1.457 fold higher than the unelicited. The highest xanthine oxidase activity was obtained by chitosan elicitation at concentration of 0.16 g L-1 and it was elicited at 1.25 day cultivation or at 30 h culture with percentage inhibition of 61.818%.
  A. Amid , R.J.M. Salim and M.I. Adenan
  The study was aimed to evaluate the metal chelating activity of CA which will first be assessed for neuroprotective property. Further, response surface methodology will be used to optimize the extraction parameters to yield an optimum metal chelating activity of CA by minimizing the cost of extraction. Centella asiatica (CA) is a rich antioxidant candidate was studied for its potential as a neuroprotective agent to fight against oxidative damage caused by Reactive Oxygen Species (ROS) towards neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer’s and Parkinson disease. The limited number of neuroprotective study carried out so far indicated that metal (iron) chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. Methanolic and aqueous extract of CA was obtained by conventional soxhlet extraction (temperature: 40-60°C, ratio: 1:30 (w/v), time: 24 h). The IC50 of methanolic and aqueous extract obtained was 0.26 and 0.69 mg mL-1, respectively. Further, Response surface methodology was used to determine the optimum CA extraction condition that gives optimum metal chelating activity. Experiments were designed according to central composite design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. In conclusion, run 17 (temperature: 25°C, ratio: 1:45 (w/v), time: 1.5 h and speed: 200 rpm) gave the optimum chelating activity with IC50 0.093 mg mL-1.
  P. Jamal , A.A. Barkat and A. Amid
  Phenolics, which are widely distributed in plant kingdom, appear to have desirable medicinal properties and play a major role in both plant and animal health. Some have been reported to be antitumor agents and to exhibit antiviral and antimicrobial activities, hypotensive effects and antioxidant properties. These compounds, either as isolates or in conjunction with other compounds, may be used for various health benefits. In this study, forty types of Malaysian medicinal plants were examined to develop an understanding of the distribution of phenolics and to give an assessment of the diversity present in the selected plants. The selection of plants was based on their frequent usages by local folks for their medicinal benefits. Total phenolic content was analyzed by Folin and Ciocalteau’s phenol reagent and their concentration was expressed as Gallic Acid Equivalent (GAE). All plant samples gave positive result with varying concentrations. Highest amount was obtained from Piper betle L. which had phenolic content of 8986.67 mg L-1 GAE, while the lowest concentration of 133.33 mg L-1 GAE was obtained from Canna indica Linn. This information can be used to assess taxonomic classifications, evaluate potential sources of phenolic compounds for agricultural and pharmaceutical uses and evaluate breeding program results.
  W.D. Wan Chik , A. Amid and P. Jamal
  This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22°C with 110 rpm within 24 h. Later, purification process was started by Thin Layer Chromatography (TLC) subjected to determine the best mobile phase for compound separation and collection by means of column chromatography. Next, the effect of purified sample towards MCF-7 breast cancer cell lines and Vero cells were observed using in vitro cytotoxicity assay to indicate its active fractions and its half maximal inhibitory concentration (IC50). Purified sample gave a rational effect towards MCF-7 breast cancer cells with IC50 value of 5.85 μg mL-1 compared to Taxol with IC50 value of 0.039 μg mL-1. The purified sample can also be judged to be harmless as it has IC50 value of 765.6 μg mL-1 in Vero cells treatment while Taxol gave IC50 value of 0.045 μg mL-1.
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