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Articles by Zuo Zhicai
Total Records ( 3 ) for Zuo Zhicai
  Zuo Zhicai , Fu Tongchao , Wan Hongping , Dai Hongbo , Chen Lei , Xu Zhiwen and Zhu Lin
  A strain named SCBI-1, isolated from a sick pig was diagnosed by cultural characteristics and morphologic observation with its pathogenicity studied by mice virulent experiment and was accurately identified by biochemical assay and the analysis of 16S rDNA sequence. Drug resistance was analysed by sensitive assay. Homology of the 16S rDNA between SCBI-1 and Bordetella bronchiseptica is 99.3-99.9%, the study successfully isolated a strain of Bordetella bronchiseptica which could lead a mouse to death within 16 h and is resistant to penicillin, rifampicin, clindamycin, ampicillin and cefalotin.
  Wan Hongping , Zuo Zhicai , Fu Tongchao , Dai Hongbo , Chen Lei , Xu Zhiwen and Zhu Lin
  A highly infectious and fatal respiratory tract disease occurred in a swine farm in Sichuan province. To explore the reason of the disease, samples of the liver and lung tissue, acquired sterilely in a typically sickened pig were intended for pathogenic detection. The suspicious strain, named after SCAB-1 was diagnosed which depended on cultural characteristics, morphologic observation and Nicotinamide Adenine Dinucleotide (NAD) experiment. Its pathogenicity was diagnosed by the virulence test on mice. And the strain was exactly identified by the analysis of 16S rDNA sequence with its resistance inspected by sensitive test. According to cultural characteristics, morphology, NAD experiment and the analysis of 16S rDNA sequence, it revealed that the study triumphantly isolated a strain of Actinobacillus pleuropneumonia which could lead a mouse to death, after infected 16 h and depend on NAD. The homology of the 16S rDNA sequence between SCAB-1 and Actinobacillus pleuropneumonia is highly 99.9%. SCAB-1, Actinobacillus pleuropneumonia is susceptible to ceftriaxone, cefotaxime, cefuroxime, norfloxacin, SMZco; moderately sensitive to cefazolin, cefoperazone and resistance to cephaloridine IV, vancomycin and gentamycin.
  Zuo Zhicai , Hongbo Dai , Lei Chen , Yuancheng Zhou , Wanzhu Guo and Zhiwen Xu
  Haemophilus parasuis mainly affect piglets during suckling period and the infected pigs showed clinical symptoms at 5-6 weeks old after weaning. To develop a specific PCR for early detection of Haemophilus parasuis, early treatment would effectively prevent phenotypic expression of the disease. A pair of specific primers were designed to amplify a 414 bp specific DNA fragment based on H. parasuis Outer Membrane Protein (OMP) gene published in GenBank also the PCR conditions were optimized. The established PCR test could detect a minimum of 425 cfu mL-1 (0.425 cfu/PCR reaction) pure cultures of H. parasuis and only H. parasuis could amplify a 414 bp specific DNA fragment. No amplification was observed while tested with the closely affiliated species and the mainly contaminating flora. From 2 days post infection (dpi) to 5 dpi, all of the piglets challenged with H. parasuis showed a fever above 41°C and all blood samples from those feverish piglets were positive for H. parasuis. Through blood PCR detection, 37 positive samples were picked out in 84 sucking pigs which were natural infected with H. parasuis, the result was verified by dissecting five positive piglets and isolating bacteria from lung tissue. The developed PCR was successfully applied for early diagnosis of H. parasuis infection, early treatment will reduce the incidence and mortality of disease.
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