Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Zuhair Muhammad Hassan
Total Records ( 1 ) for Zuhair Muhammad Hassan
  Gholamreza Zarrini , Zargham Sepehrizadeh , Mojtaba Tabatabaei Yazdi , Qorbane Behzadiyan Nejad and Zuhair Muhammad Hassan
  Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility